FastQCFastQC Report
Mon 28 Nov 2022
4061-JW18trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW18trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243218
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA9657739.707998585630996No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATAGCCCAGCA93363.8385316876218045No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA80613.3143106184575153No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT72662.9874433635668414No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA67992.795434548429804No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA65172.679489182544055No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG63052.5923245812398754No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG49612.0397339012737543No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT31471.2939009448313858No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG29221.201391344390629No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT20630.8482102475968062No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA18840.7746137210239373No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC13240.544367604371387No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG11790.48475030630956595No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG10700.4399345443182659No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA10330.4247218544680081No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA9880.40621993437985676No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT9390.38607339917275857No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA9160.3766168622388146No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATGTCGGTCCAGTA8670.3564703270317164No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT8590.3531810967938228No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA7920.3256337935514641No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA7450.30630956590383934No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA7130.293152644952265No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC7100.29191918361305497No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT7010.2882187995954247No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA6820.28040687778042744No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT6300.2590268812341192No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA6130.2520372669785953No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG6050.24874803674070173No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG5980.24586996028254487No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA5960.2450476527230715No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG5930.24381419138386137No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG5880.2417584224851779No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA5710.23476880822965404No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA5640.2318907317714972No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA5420.22284534861728983No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA5350.21996727215913295No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC5320.21873381081992288No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT5270.21667804192123935No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCG4940.20310996718992838No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG4910.20187650585071826No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA4680.19241996891677426No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA4630.19036420001809076No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT4410.18131881686388343No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA4110.16898420347178253No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA4050.16651728079336234No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA3930.16158343543652198No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA3840.1578830514188917No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA3660.15048228338363115No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA3600.14801536070521096No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG3500.14390382290784398No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT3440.14143690022942382No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3190.1311580557360064No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG3130.1286911330575862No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT3120.1282799792778495No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA3040.12499074903995593No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA2940.12087921124258895No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA2910.11964574990337887No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT2910.11964574990337887No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGAA2840.11676767344522199No Hit
CCCGTTCGCTACCCATGCTTTCGCGTCTTAGCGTCAGGTACAGCCCAGGG2800.1151230583262752No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACAGTCCAGGA2760.1134784432073284No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA2730.11224498186811832No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTGCGCCAGTG2690.11060036674917152No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA2690.11060036674917152No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTCTTGGTCCAGAA2680.11018921296943483No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTCGTCCAGAA2680.11018921296943483No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2610.10731113651127795No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTATAGCTTAGCT2600.10689998273154125No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2570.10566652139233117No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCTAGGA2540.10443306005312107No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG2480.1019661373747009No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA2450.1007326760354908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTGC202.3296525E-7226.04343
TGTTTGT100.0018777414226.04343
TGTTTGA6250.0226.04343
GTTTGCG100.0018777414226.04344
ATTCGAT100.0018777414226.04344
GTTCGAT10100.0226.04344
TCGCTGC152.0930724E-5226.04346
TGTTCGC4350.0226.04343
TGTTCGA10050.0226.04343
CTGTTCG14400.0226.04342
CTATTTG202.3296525E-7226.04342
TTTGCTG100.0018777414226.04345
TTTTGCT152.0930724E-5226.04344
TGTGTGC202.3296525E-7226.04343
ATTTGCT202.3296525E-7226.04344
CGATCCC10200.0226.043387
GTTTGCT187700.0225.68214
CTGTTTG186000.0225.67882
TGTTTGC179600.0225.665823
CCTGTTT186050.0225.618161
Produced by FastQC (version 0.11.9)