FastQCFastQC Report
Mon 28 Nov 2022
4061-JW19trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW19trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169414
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA3054918.03215790902759No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1773110.466077183703826No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA136498.056595086592608No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA133417.874791929828703No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA54843.237040622380677No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCATGCA46512.7453457211328463No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA40582.395315617363382No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA38422.267817299632852No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA35682.106083322511717No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA30871.8221634575654906No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA21801.2867885770951633No Hit
GACGGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCCGCA19061.125054599974028No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTTACTGGGCGTAAAGGGCCCGTA18601.0979021804573412No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA18471.0902286705939297No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA17071.0075908720648825No Hit
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGTA16350.9650914328213726No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA14860.8771412043868866No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA11600.6847131878121052No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA8810.520027860743504No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA8260.48756301132137836No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA7720.45568843188874586No Hit
TTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTA7290.4303068223405386No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA6400.3777727932756443No Hit
TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA6360.3754117133176715No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA6130.36183550355932803No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA6100.3600646935908485No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA5990.3535717237064233No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA5890.3476690238114914No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA5870.34648848383250497No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA5590.32996092412669553No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGCGCA5480.32346795424227043No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA5300.3128430944313929No Hit
TACGAGAGGGGCAAGCGTTATTCATCATAACTGGGCGTAAAGTGTCCGTA5270.31107228446291335No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA4400.2597187953770055No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA3850.22725394595487974No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3590.2119069262280567No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTCACTGGGTATAAAGGGTGCGCA3510.2071847663121112No Hit
GACGGGGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA3360.19833071646971323No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA3220.1900669366168085No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA2910.1717685669425195No Hit
TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2850.16822694700556035No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA2680.15819235718417604No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA2420.14284533745735298No Hit
TACAGAGGGAGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTTTGTA2330.13753290755191425No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2330.13753290755191425No Hit
TACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA2260.1334010176254619No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2160.12749831773052994No Hit
TACAGAGGTGGCGAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA2110.12454696778306398No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGCA2100.12395669779357078No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCATGCA2040.12041507785661161No Hit
TACGTAGGACGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA2020.11923453787762521No Hit
CACGTAGGCACCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA2020.11923453787762521No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1950.11510264795117287No Hit
CACGTAAGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTACGTA1880.1109707580247205No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA1880.1109707580247205No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1800.10624859810877496No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGTA1780.10506805812978857No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA1780.10506805812978857No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1750.10329724816130897No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA1750.10329724816130897No Hit
TACGAGATGTGCAAGCGTTGTTCGGAATTACTGGGCATAAAGGGTGCGCA1730.10211670818232259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAG1250.0226.9959
CTCCAAT1250.0226.9957
ACGAACC650.0226.9952
AACGTAG2100.0226.994981
CGAACCA252.5393092E-9226.994983
GATCCAA152.0571877E-5226.994988
GGTCCCG750.0226.994987
CACGAGG152.0571877E-5226.994981
AGACGAG750.0226.994989
AGGCCTC152.0571877E-5226.994986
TACGAAG18600.0226.994981
CACGTAA350.0226.994981
AGGTCTC252.5393092E-9226.994986
GCCCAAG202.2793392E-7226.994969
GTACAAA100.0018537816226.994969
GGCACCA100.0018537816226.994967
GACGTAG202.2793392E-7226.994961
AACCACC202.2793392E-7226.994965
AGCCAAG100.0018537816226.994969
AAGGTTG202.2793392E-7226.994965
Produced by FastQC (version 0.11.9)