FastQCFastQC Report
Mon 28 Nov 2022
4061-JW19trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW19trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169414
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1890911.161415231326808No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG123837.309313279894224No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT123587.294556530156894No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA115336.807583788825008No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA104326.157696530393002No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA56173.315546530983272No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG48532.8645802590104714No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATGTCGGCCCGGCG41262.4354539766489194No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA40892.413613987037671No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTTGGCCAGAA34432.0322995738250675No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT30111.7773029383640078No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA21511.2696707473998607No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT19951.1775886290389224No Hit
CCGGTTCGCTCCCCACGCTTTCGCCCCTCAGCGTCAGGTCAGGCCCAGGT19781.167554039217538No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT18401.0860967806674773No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA17341.0235281617811989No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGC16600.9798481825587023No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG16340.9645011628318793No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG16310.9627303528633998No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT11790.695928317612476No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG9920.5855478295772486No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG9660.5702008098504255No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTTGGCCCAGAT8620.5088127309431334No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG8120.4792992314684737No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGGA6500.38367549317057625No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT6410.37836306326513747No Hit
CGTCTTCGAGCCCCCAACTTTCGTTCTTGATCAATGAAAACATCCTTGGC6020.3553425336749029No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA5830.3441274038745322No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGA5780.34117605392706624No Hit
CCTGTTTGCTACCCATACTTTCGTCCCTCAACGTCAGTTTATACTAAAGA5660.33409281405314795No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATATATGTGTAGCC5450.3216971442737908No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG5350.3157944443788589No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT5210.3075306645259542No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT5020.2963155347255835No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGA4940.29159337480963793No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG4660.2750658151038285No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT4640.2738852751248421No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG4610.2721144651563625No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA4320.2549966354610599No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT4020.23728853577626408No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC4020.23728853577626408No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGCCTGAGCGTCAGTTCCGGGCCAGAA3790.2237123260179206No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTCTCTGGCCAGGG3620.2136777361965363No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC3590.2119069262280567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG3200.18888639663782214No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG3160.18652531667984937No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT3090.18239342675339698No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3050.18003234679542424No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGGT2950.1741296469004923No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG2910.1717685669425195No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT2780.164095057079108No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA2600.15347019726823047No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG2560.15110911731025772No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGT2550.1505188473207645No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCGGTATCGCGCCAGAT2530.1493383073417781No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTCAGTAATAGCCCAGTA2480.14638695739431215No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA2380.14048425749938023No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA2370.13989398750988702No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG2330.13753290755191425No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG2210.13044966767799593No Hit
CCTATTCGCTCCCCCAGCTTTCGTTTCTCAGTGTCAGTCTCGGCCCAGTA2170.12808858772002313No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2170.12808858772002313No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT2120.12513723777255717No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATAACGGGCCAGAT2090.1233664278040776No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGCAATTTAGTA2090.1233664278040776No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2030.11982480786711842No Hit
CCTGTTTGCTCCCCTTGCTTTCGCGCCTCAGCGTCAGTTTCGTTCCAGGG2010.11864426788813204No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA1970.11628318793015925No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGACCAGGA1920.11333183798269328No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATGAGCGTCAGAAATGCCCCAGCA1920.11333183798269328No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCA1920.11333183798269328No Hit
CCTGTTTGCTACCCATACTTTCGTTCATCAACGTCAGTTTTTACTAGAGA1890.1115610280142137No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT1870.11038048803522732No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGCATGTTCCAGAG1870.11038048803522732No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA1860.1097902180457341No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAATCGACGGCCAGGT1710.10093616820333622No Hit
CCCATTCGCTCCCCTGGCTTTCGTTCCTCAGCGTCAGTAATAGCCCAGCA1700.10034589821384302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTGC202.3299435E-7225.997363
GTTTGCG100.0018783852225.997364
TTGGCTA252.6066118E-9225.997365
CCGATTC152.0936563E-5225.997361
CAATTTG100.0018783852225.997362
TGGTTGC100.0018783852225.997363
CCTTTTG252.6066118E-9225.997362
TTTGGCT252.6066118E-9225.997364
CTATTTG202.3299435E-7225.997362
CTATTCG152.0936563E-5225.997362
TTTGCTT100.0018783852225.997365
GGTTGCT100.0018783852225.997364
CCTGGTT100.0018783852225.997361
CCTATTT202.3299435E-7225.997361
GGTTCGT100.0018783852225.997363
TGGCTAC252.6066118E-9225.997366
TGTTTGA2750.0225.997343
CTGTTCG6750.0225.997342
TCGATCC6250.0225.997336
TGTTCGA5450.0225.997333
Produced by FastQC (version 0.11.9)