FastQCFastQC Report
Mon 28 Nov 2022
4061-JW20trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW20trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128357
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA3500927.27471037808612No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA2085316.246094876009877No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA1323610.311864565236021No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT40103.1240991920970416No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA12060.939566988944896No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT10460.8149146521031186No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG10030.7814143365768911No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGTGTCAGTTCAGACCCAGAT7610.592877677103703No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA6720.5235398147354644No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA6670.5196444292091589No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA6280.4892604221039756No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA5790.4510856439461814No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA5770.44952748973565915No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT5320.41446901999890934No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA5230.4074573260515593No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG5110.398108400788426No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA5030.39187578394633715No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGTGTCAGGATCAGTCCAGAA4840.37707331894637613No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA4190.3264333071044041No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAATATTGGCTTTGTG4000.3116308421044431No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTTGGCCAGAA3970.30929361078865975No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGGACCCGTTCTGGTG3970.30929361078865975No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG3870.3015028397360487No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA3650.2843631434203043No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG3140.2446302110519878No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2940.22904866894676565No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA2920.22749051473624346No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA2910.22671143763098234No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGTGTCAGAAACAGTCCAGGA2810.21892066657837125No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA2750.2142462039468046No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2660.20723450999945467No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC2600.20256004736788802No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGTGTCAGTTACAGTTTAGTA2560.19944373894684359No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA2490.1939901992100158No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA2420.18853665947318807No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG2330.18152496552583808No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG2240.17451327157848812No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1970.1534781897364382No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT1960.1526991126311771No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGAT1950.15192003552591601No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1950.15192003552591601No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1900.14802464999961046No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1800.14023387894699937No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT1770.13789664763121606No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA1770.13789664763121606No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA1750.13633849342069385No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1740.13555941631543275No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA1730.13478033921017163No Hit
CCCCTTTGCTCCCCACGCTTTCGCGCTTCAGCGTCGGTAGCAGGCCAGAG1680.1308849536838661No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATCGGCCCAGGG1650.12854772236808276No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTTCGGTCCAGGA1630.12698956815756055No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG1630.12698956815756055No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1540.11997787421021058No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA1520.11841971999968838No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1510.11764064289442726No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1500.11686156578916615No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA1490.11608248868390506No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1480.11530341157864393No Hit
CCTGTTTGCTACCCACACCTTCGCGCATCAGCGTCAGTATTGGTCCAGGA1450.11296618026286061No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG1440.1121871031575995No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA1430.1114080260523384No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA1340.10439633210498843No Hit
CCCATTCGCTACCCTAGCTTTCGCGTCTTAGCGTCAGAAACGGTCCAGAA1320.10283817789446621No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1310.1020591007892051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA2950.0226.000023
GGTTTGC252.6029738E-9226.000023
TTTGCGC252.6029738E-9226.000025
CGTTTGC6400.0226.03
CGTCATA100.0018777908226.02
CGTTTAC100.0018777908226.03
TTTTGCT152.09238E-5226.04
TTTTGCG100.0018777908226.04
GTTTGCG152.09238E-5226.04
GTTTGAT3200.0226.04
GTCATAA100.0018777908226.03
CGCCCCA202.3276152E-7226.09
ACGTCAT100.0018777908226.01
CGCTTCG100.0018777908226.02
TGTTCGC1600.0226.03
TGTTCGA3050.0226.03
TTTTTGC100.0018777908226.03
TGTTTAC100.0018777908226.03
CCGTTTA100.0018777908226.02
GTTCACT100.0018777908226.04
Produced by FastQC (version 0.11.9)