FastQCFastQC Report
Mon 28 Nov 2022
4061-JW21trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW21trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149955
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA7351949.027374879130406No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA35472.365376279550532No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA24161.6111500116701678No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTCATTGGGCGTAAAGCGAATGTA21731.4491013970857924No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA21071.405088193124604No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA19981.332399719915975No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA18941.263045580340769No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA11720.7815678036744357No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA11280.7522256677003101No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA10940.7295521989930313No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10470.6982094628388517No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA7620.5081524457337202No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA7350.49014704411323395No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA7180.47881030975959454No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA7090.4728085092194325No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5820.38811643493047915No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA5710.3807809009369478No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA5130.34210263078923675No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5060.33743456370244407No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA4940.329432162982228No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA4500.30009002700810244No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA4480.2987562935547331No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA4430.2954219599213097No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA4350.2900870261078323No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA4330.288753292654463No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA3800.2534093561401754No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3790.2527424894134907No Hit
TACAGAGACCTCAAGCGTTATCCGGATTAATTGGGCGTAAAGCGCAGGTA3660.24407322196658998No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3440.2294021539795272No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA3180.2120636190857257No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA3130.20872928545230238No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA3120.20806241872561768No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA3110.207395551998933No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA2890.19272448401187023No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA2880.19205761728518556No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA2850.19005701710513154No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2840.18939015037844686No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2800.18672268347170817No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2780.18538895001833883No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA2680.1787202827514921No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA2660.17738654929812278No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA2580.1720516154846454No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2450.16338234803774465No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA2360.1573805474975826No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2340.15604681404421328No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA2330.15537994731752858No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2310.15404621386415923No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA2210.14737754659731253No Hit
CACCGGCGGCTCGAGTGGTAACCGTTATTATTGGGTCTAAAGGGTCTGTA2190.14604381314394319No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA2100.14004201260378113No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2000.13337334533693443No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1980.13203961188356506No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA1860.124037211163349No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1850.12337034443666434No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA1840.12270347770997965No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1840.12270347770997965No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCGTGCA1820.12136974425661032No Hit
CACCGGCGGCCCGAGTGGTGATCGTGATTATTGGGTCTAAAGGGTCCGTA1800.12003601080324097No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1800.12003601080324097No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA1790.11936914407655629No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1780.11870227734987163No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA1740.11603481044313294No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGAGCA1640.1093661431762862No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA1600.10669867626954754No Hit
GACAGAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGCGAACGCA1600.10669867626954754No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA1580.10536494281617818No Hit
TACAGGGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA1570.1046980760894935No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1560.10403120936280884No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA1530.10203060918275483No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGTA1530.10203060918275483No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGCAAGCA1520.10136374245607015No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1500.10003000900270081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA302.910383E-11226.992687
CCTCAAA152.0568017E-5226.992689
TTGCGAG152.0568017E-5226.992689
ACCGTCC302.910383E-11226.992686
GCAGCAA152.0568017E-5226.992688
AGGAGTC100.0018536268226.992666
GGATGCT100.0018536268226.992667
CAGCACC100.0018536268226.992664
GATCCAA350.0226.992668
GAATCGA202.2785935E-7226.992668
GGATCCA350.0226.992667
ATGCTAG100.0018536268226.992669
GTAGGAT3350.0226.992664
AGACGAG252.5374902E-9226.992669
GCCTCAA100.0018536268226.992668
GATGCTA100.0018536268226.992668
GGTGCTA550.0226.992668
GAGCCAA2700.0226.992668
CGTAAGG202.2785935E-7226.992663
ACGTATG850.0226.992662
Produced by FastQC (version 0.11.9)