FastQCFastQC Report
Mon 28 Nov 2022
4061-JW22trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW22trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97031
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA3974640.96216673022024No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA24322.5064154754666035No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG22102.2776226154527937No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG20482.110665663550824No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA16331.6829672991105933No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA15841.6324679741525905No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT15011.5469283012645445No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT10541.0862507858313322No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA8080.832723562572786No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC6700.6905009739155528No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT6100.6286650658037123No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA5320.5482783852583195No Hit
CCTGTTCGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT4300.4431573414681905No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA4100.4225453720975771No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT4050.4173923797549237No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA3980.410178190475209No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA3830.3947192134472488No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG3820.39368861497871815No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA3810.39265801651018745No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG3740.3854438272304727No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG3690.38029083488781934No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3650.3761684410136966No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA3600.37101544867104325No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA3550.3658624563283899No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC3290.3390668961465923No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3220.33185270686687757No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA3170.3266997145242242No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA3110.32051612371304017No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC3080.3174243283074481No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA3060.31536313137038674No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA3030.3122713359647948No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT2920.30093475281095733No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG2720.2803227834403438No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA2520.2597108140697303No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2460.25352722325854626No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA2410.24837423091589286No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG2340.24116004163617813No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT2320.23909884469911677No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT2290.23600704929352476No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2220.22879286001381No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA2220.22879286001381No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG2160.22260926920262594No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG2140.22054807226556458No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG2100.21642567839144194No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA2100.21642567839144194No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA2090.21539507992291126No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2010.20715029217466585No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1990.2050890952376045No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA1980.2040584967690738No Hit
CCCATTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAATGGTCCAGGA1930.19890550442642044No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1910.19684430748935908No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1900.1958137090208284No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1820.18756892127258298No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA1800.18550772433552162No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATCACGGCCCGGCG1800.18550772433552162No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA1730.17829353505580692No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT1690.1741711411816842No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG1670.17210994424462286No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCAACCAGAA1620.16695695190196946No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1600.16489575496490813No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA1480.15252857334254002No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTGTCGGTCCAGGA1440.1484061794684173No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGTTTAGTA1440.1484061794684173No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA1440.1484061794684173No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA1400.1442837855942946No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA1390.14325318712576393No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA1330.13706959631457988No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA1320.1360389978460492No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTGCGCCAGTG1210.12470241469221177No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA1210.12470241469221177No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAGTGGCCCAGGG1190.12264121775515041No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1190.12264121775515041No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG1180.12161061928661974No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCGTTCCAGAA1180.12161061928661974No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA1150.1185188238810277No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA1140.11748822541249704No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACGGGCCCAGCG1110.114396430006905No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT1100.11336583153837433No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGGCCAGGA1050.10821283919572097No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1040.10718224072719029No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGGA1040.10718224072719029No Hit
CCTGTTTGCTCCCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA1020.10512104379012893No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCCTCGAATAGAA1010.10409044532159825No Hit
CCCGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGCTGGGCCAGTG1010.10409044532159825No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA1000.10305984685306759No Hit
CCTGTTTGCTCCCCATGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAT990.1020292483845369No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG980.10099864991600623No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAACGGCCCAGCA980.10099864991600623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGC1100.0226.000023
TTGCTCC68800.0226.000026
GTTTGCT77100.0226.04
GTTTGCG152.0908099E-5226.04
GTTTGAT2000.0226.04
TTCGCTG100.0018770855226.05
TCGATCC3150.0226.06
TGTCTGC152.0908099E-5226.03
TGTTTGA2000.0226.03
GGTTTGC202.3247048E-7226.03
GTTCACT100.0018770855226.04
GCTACCT100.0018770855226.08
TCGCTGC100.0018770855226.06
TGCTACC8400.0226.07
CGGTTTG202.3247048E-7226.02
TTTGCTC68750.0226.05
TTTGCGC202.3247048E-7226.05
GTTCGCG100.0018770855226.04
TTTGATC2000.0226.05
TGTTCGC2700.0225.999983
Produced by FastQC (version 0.11.9)