FastQCFastQC Report
Mon 28 Nov 2022
4061-JW25trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW25trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112320
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA1744915.535078347578349No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA110629.848646723646723No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA94088.376068376068375No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA84997.566773504273503No Hit
GACGGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCCGCA40253.5835113960113962No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA35223.1356837606837606No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA21941.9533475783475782No Hit
TACGGAGGGGGCGAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA20871.8580840455840457No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA16831.498397435897436No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCATGCA16661.4832621082621082No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA12881.1467236467236468No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGCGCA12031.0710470085470085No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA11371.0122863247863247No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA11170.9944800569800569No Hit
TACGGAGGAGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTA10390.9250356125356126No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA10020.892094017094017No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA8630.7683404558404558No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA8180.7282763532763532No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA7740.6891025641025641No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTTACTGGGCGTAAAGCGCGTGTA7390.6579415954415955No Hit
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGTA7150.6365740740740741No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA7020.625No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA6510.5795940170940171No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA5030.4478276353276353No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTTACTGGGCGTAAAGAGCACGTA4990.4442663817663818No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA4930.4389245014245014No Hit
TACGTAGGACGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA4320.38461538461538464No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGCA4000.3561253561253561No Hit
TACGTATGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGCA3940.35078347578347574No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA3830.34099002849002846No Hit
TACGAAGGTGGCGAGCGTTATTCGGAATCACTGGGCTTAAAGCGCATGTA3680.32763532763532766No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA3390.30181623931623935No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA3350.29825498575498577No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA3260.29024216524216523No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA3150.28044871794871795No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA3040.2706552706552707No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA2730.24305555555555555No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTTACTGGGCGTAAAGGGCCCGTA2580.22970085470085472No Hit
TACAGAGGCCACAAGCGTTAGGCGGAATCACTGGGCTTAAAGCGTGTGTA2570.2288105413105413No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA2200.19586894586894588No Hit
TACAGAGAGTGCAAACGTTGTTCGGAATTACTGGGCTTAAAGCGCGCGTA2140.19052706552706553No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA2120.18874643874643876No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA2030.18073361823361822No Hit
TACGAAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA1960.1745014245014245No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA1940.1727207977207977No Hit
TACAGAGGGTGCGAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA1850.16470797720797722No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA1800.16025641025641024No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA1690.15046296296296297No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA1670.14868233618233617No Hit
TACCGGCGGCTCGAGTGGTGGCCGCTTTTACTGGGCTTAAAGGGTCCGTA1660.1477920227920228No Hit
TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA1640.146011396011396No Hit
TACGGAGGATCCTAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA1640.146011396011396No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1620.14423076923076925No Hit
CACGTAAGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTACGTA1600.14245014245014245No Hit
TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA1590.14155982905982906No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1560.1388888888888889No Hit
TACGAAGGGTGCAAGCGTTGCTCGGAATTATTGGGCGTAAAGGGTAGGTA1540.1371082621082621No Hit
TACTCGCGGTCCAAGTCGCAGCCACAATTATTGGGTCTAAAACATCCGTA1530.1362179487179487No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA1500.13354700854700854No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTACAGGGTGCGTA1470.13087606837606836No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA1440.1282051282051282No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1400.12464387464387464No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCTCGTA1350.1201923076923077No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA1310.11663105413105414No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA1300.11574074074074073No Hit
TACGTAGGCAGCAAGCGTTGTTCGGAGTTACTGGGCGTAAAGAGTACGTA1300.11574074074074073No Hit
GACAGAGGGTGCAAACGTTGCTCGGAATCACTGGGCATAAAGCGCACGTA1290.11485042735042736No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA1230.10950854700854701No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1190.10594729344729345No Hit
TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1190.10594729344729345No Hit
TACGAGATGTGCAAGCGTTGTTCGGAATTACTGGGCATAAAGGGTGCGCA1180.10505698005698007No Hit
GACAGAGGTTGCAAACGTTGTTCGGAATCACTGGGCATAAAGCGCACGTA1170.10416666666666667No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1160.10327635327635329No Hit
GACGGAGGATGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGCA1140.10149572649572651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAA100.0018528295227.000028
ACTGTGC650.0227.000026
AACCGTC202.2756831E-7227.000025
ACACGAG100.0018528295227.000029
AGCCAAG202.2756831E-7227.000029
ACGTCGG100.0018528295227.000022
ACCACCC202.2756831E-7227.000026
ACCGTCC202.2756831E-7227.000026
ACGTACT100.0018528295227.000022
GAGCCAA202.2756831E-7227.000028
GGACACG100.0018528295227.000027
GTACAAA202.2756831E-7227.000029
CACGAAC100.0018528295227.000021
CCGTCCA202.2756831E-7227.000027
TACATAG100.0018528295227.000021
CCACCCA202.2756831E-7227.000027
AACCACC202.2756831E-7227.000025
TACGTCG100.0018528295227.000021
ACATAGG100.0018528295227.000022
GAACCGT500.0227.000024
Produced by FastQC (version 0.11.9)