FastQCFastQC Report
Mon 28 Nov 2022
4061-JW25trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW25trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112320
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1611314.345619658119658No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT68186.070156695156695No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA67115.974893162393163No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG50014.452457264957265No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGC36733.2701210826210825No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA32732.9139957264957266No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT18791.6728988603988602No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATGTCGGCCCGGCG18301.6292735042735045No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG16401.46011396011396No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG15631.391559829059829No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG14101.2553418803418803No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT13801.2286324786324787No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT11241.0007122507122508No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT11241.0007122507122508No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA11150.9926994301994303No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT10710.9535256410256411No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA10180.9063390313390313No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG10170.9054487179487181No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT8310.7398504273504273No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTTGGCCAGAA8150.725605413105413No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT8090.7202635327635327No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAATCGACGGCCAGGT7850.6988960113960113No Hit
CCGGTTTGCTCCCCACGCTGTCGCGTCTCAGTGTCAGAACAGGGCCAGGT7490.6668447293447294No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG7310.6508190883190883No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA7090.6312321937321937No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA7080.6303418803418803No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA6950.6187678062678063No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG6260.5573361823361823No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG5710.5083689458689459No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA5630.5012464387464387No Hit
CCGGTTCGCTCCCCACGCTGTCGCACCTCAGCGTCAGGTCAGGCCCAGGT5360.4772079772079772No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG5160.45940170940170943No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT4390.39084757834757833No Hit
CCTGTTTGCTACCCATACTTTCGTTCATCAACGTCAGTTTTTACTAGAGA4360.38817663817663817No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTCTCCGTCCAGCA4210.3748219373219373No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCGGTATCGCGCCAGAT4200.37393162393162394No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG4100.36502849002849No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGCAATTTAGTA4000.3561253561253561No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTCAGCGTCAGTACAAGCCCAGCG3780.33653846153846156No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATAACGGCCCGGCG3650.3249643874643875No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTTAGTGTCAGGTCAGGCCCAGGT3580.31873219373219375No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG3520.31339031339031337No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT3170.2822293447293447No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG2840.25284900284900286No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG2710.24127492877492876No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG2700.2403846153846154No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA2540.22613960113960113No Hit
CCGGTTCGCTCCCCACGCTTTCGCCCCTCAGCGTCAGGTCAGGCCCAGGT2440.21723646723646722No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT2370.2110042735042735No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2250.2003205128205128No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC2210.19675925925925924No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG2120.18874643874643876No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2120.18874643874643876No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGT2050.182514245014245No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG2040.18162393162393162No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG2020.17984330484330485No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTTGGCCCAGAT2000.17806267806267806No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGA1960.1745014245014245No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT1950.1736111111111111No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG1910.17004985754985755No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT1840.16381766381766383No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT1790.15936609686609687No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1770.15758547008547008No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTGCCGTCCAGGT1750.15580484330484332No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTATGGACCAGAG1750.15580484330484332No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG1710.15224358974358976No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGCGTCAGTACCTGTCCAGGT1690.15046296296296297No Hit
CCGTTTTGCGCCCCTAGCCTTCATCCCTTACTGTCAGAACTGTCCTCGTA1670.14868233618233617No Hit
CCTGTTTGCTCCCCACGCTTTCGTAGTTGAGTGTCAGTATCTGTCCAGGT1660.1477920227920228No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTGTCCAGGG1570.13977920227920226No Hit
CCCGTTCGCTACCCTAGCTTTCGTGCCTCAGCGTCAGACAAGGCCCAGTG1560.1388888888888889No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA1500.13354700854700854No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1480.13176638176638178No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1470.13087606837606836No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATCGACCCAGGG1440.1282051282051282No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGACAGGCCCAGGT1440.1282051282051282No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGTGTCAGACATCGTCCAGAA1440.1282051282051282No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT1420.12642450142450143No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA1400.12464387464387464No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGTGTCAGTTAAGCCCCAGTG1390.12375356125356127No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATGAGCGTCAGAAATGCCCCAGCA1340.1193019943019943No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG1320.11752136752136752No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1320.11752136752136752No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG1280.11396011396011395No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1260.11217948717948717No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGAGCCAGTG1250.11128917378917379No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGAAGTTGCCCAGTG1240.11039886039886039No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG1240.11039886039886039No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1230.10950854700854701No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCATCAGTGTCAGTTATGGCCCAGTA1230.10950854700854701No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG1200.10683760683760685No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGAA1200.10683760683760685No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCTTAGTT1170.10416666666666667No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATGTCGGCCCGGTG1170.10416666666666667No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT1150.10238603988603988No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGTGACGGTCCAGTG1150.10238603988603988No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA1130.1006054131054131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC2950.0226.000026
GGTTTGC1450.0226.000023
CCATTCG4950.0226.000022
CGGTTTG1450.0226.000022
TTTGCGC450.0226.000025
TTTTGCT302.910383E-11226.04
ATCTTCG302.910383E-11226.02
TGTTCGC1150.0226.03
TGTTCGA2600.0226.03
CCTTTTG252.6011548E-9226.02
ATTTGCT19600.0226.04
CTTTGCT350.0226.04
CTGTTCG3750.0226.02
TTTGCTC43700.0226.05
CCATTTG19550.0225.999982
CGTTTGA100.0018774791225.999973
TGTTTTC100.0018774791225.999973
GTTTTGC400.0225.999973
TCGCTGC100.0018774791225.999976
TTGGCTA100.0018774791225.999975
Produced by FastQC (version 0.11.9)