FastQCFastQC Report
Mon 28 Nov 2022
4061-JW26trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW26trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144401
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA6033241.78087409366971No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA137579.526942334194361No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA99796.91061696248641No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT31322.1689600487531253No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG14691.0173059743353579No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA12320.8531796871212802No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG11220.7770029293425946No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA10470.725064230857127No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA9780.677280628250497No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT7940.5498576879661498No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA7330.5076142131979695No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA6880.476450994106689No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA5020.34764302186272944No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT4680.32409747854931753No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA4660.32271244658970505No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT4610.3192498666906739No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA4050.28046897182152475No Hit
CCCATTCGCTACCCTAGCTTTCGCGTCTTAGCGTCAGAAACGGTCCAGAA3770.26107852438695023No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA3760.260386008407144No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA3470.2403030449927632No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA3220.22299014549760737No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3170.21952756559857617No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3110.2153724697197388No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA3050.2112173738409014No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT3000.2077547939418702No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA2940.20359969806303282No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG2940.20359969806303282No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGTGTCAGAAACAGTCCAGGA2850.1973670542447767No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGGACCCGTTCTGGTG2850.1973670542447767No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA2810.19459699032555175No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA2790.1932119583659393No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2780.19251944238613306No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC2770.19182692640632681No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA2680.1855942825880707No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG2650.183516734648652No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG2610.1807466707294271No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA2590.17936163876981462No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2540.17589905887078344No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGTGTCAGGATCAGTCCAGAA2430.16828138309291488No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG2400.16620383515349618No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA2400.16620383515349618No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA2350.162741255254465No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2290.1585861593756276No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA2250.15581609545640265No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA2220.15373854751698396No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA2200.15235351555737148No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA2050.14196577586027798No Hit
CCCGTTCGCTACCCTAGCTTTCGAGTCTGAGTGTCAGGATCAGACCAGGG2030.1405807439006655No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTACAGCTTAGTG1980.13711816400163432No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1950.13504061606221562No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGTGTCAGTTCAGACCCAGAT1930.13365558410260317No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1890.13088552018337823No Hit
CCCATTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAATGGTCCAGGA1810.12534539234492836No Hit
CCTGTTTGCTCCCCTAGCTTTCTCATTTCAGCGTCAGTATCGGACCAGTA1770.12257532842570341No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA1730.11980526450647848No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1710.11842023254686601No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT1660.11495765264783486No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1600.11080255676899743No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGAT1540.10664746089016003No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1540.10664746089016003No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTACCGGACCAGAA1470.10179984903151641No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG1460.10110733305171017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTT152.0930225E-5225.997241
TGTTCGC2150.0225.997243
TGTTTGA2350.0225.997243
GGTTCGT302.910383E-11225.997243
GGTTCGC1900.0225.997243
CCATTCG2150.0225.997242
CTTTGCT152.0930225E-5225.997244
CCGGTTC2200.0225.997241
GTTCGTG302.910383E-11225.997244
TTTTGCT100.0018781022225.997224
GTTTGAT2500.0225.997224
TGTTCGA3150.0225.997223
GGTTTGC252.6047928E-9225.997223
TGATACC202.3287612E-7225.997227
TGCCCCT100.0018781022225.997229
TTGATAC202.3287612E-7225.997226
ATTTGCT202.3287612E-7225.997224
TTTGCTT100.0018781022225.997225
GTTCGAT3150.0225.997224
TTTGATC2300.0225.997225
Produced by FastQC (version 0.11.9)