FastQCFastQC Report
Mon 28 Nov 2022
4061-JW28trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW28trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130203
Sequences flagged as poor quality0
Sequence length233
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA5511742.331589901922385No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA37542.8831900954663103No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA27012.0744529695936342No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA24111.8517238466087569No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA18251.4016574118875909No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA16381.2580355291352734No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA13511.0376105005261016No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA13241.016873651144751No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA12170.9346942850779169No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA11280.8663394852653165No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA9180.7050528789659225No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA9010.6919963441702572No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA8900.6835479981260033No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA8460.6497546139489874No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA6640.5099728884895125No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA5790.44469021451118634No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA5570.4277935224226784No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA5300.4070566730413278No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA5090.39092801241138836No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA4990.38324769782570295No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4970.38171163490856586No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA4920.37787147761572315No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA4750.3648149428200579No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA4670.35867069115150957No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4660.35790265969294105No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA4490.3448461248972758No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA4480.34407809343870727No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4100.3148928980131026No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA4030.3095166778031228No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3950.3033724261345745No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3920.3010683317588689No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA3710.28493967112892943No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA3630.2787954194603811No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA3600.2764913250846755No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA3030.2327135319462685No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA2760.21197668256491786No Hit
CACGTAAGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTACGTA2740.21044061964778077No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2680.20583243089636952No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA2660.20429636797923245No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA2640.20276030506209533No Hit
TACAGAGACCTCAAGCGTTATCCGGATTAATTGGGCGTAAAGCGCAGGTA2630.20199227360352678No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2570.19738408485211553No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA2530.19431195901784137No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA2530.19431195901784137No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA2510.19277589610070428No Hit
AACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA2500.19200786464213573No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA2450.18816770734929303No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2430.18663164443215594No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2410.18509558151501884No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA2360.18125542422217614No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2350.18048739276360762No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2340.17971936130503904No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA2340.17971936130503904No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2340.17971936130503904No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA2300.17664723547076489No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA2260.17357510963649073No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2170.16666282650937383No Hit
TACGTAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGGGTCCGCA2030.15591038608941424No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGCAAGCA1890.1451579456694546No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1880.14438991421088607No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA1830.14054975691804336No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1820.13978172545947481No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCGTGCA1770.1359415681666321No Hit
GACGGAGGGTGCTAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA1770.1359415681666321No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGAGCA1730.13286944233235792No Hit
CACGTAGGAGTCTAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGTA1690.12979731649808376No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1670.12826125358094667No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1650.12672519066380958No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1630.1251891277466725No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCACGTGTA1620.12442109628810397No Hit
CACCGGCGGCCCGAGTGGTGATCGTGATTATTGGGTCTAAAGGGTCCGTA1570.12058093899526125No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA1570.12058093899526125No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1560.11981290753669269No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1530.11750881316098707No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA1500.11520471878528145No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1470.11290062440957582No Hit
CACCGGCGGCTCGAGTGGTAACCGTTATTATTGGGTCTAAAGGGTCTGTA1460.11213259295100728No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA1430.10982849857530165No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1410.10829243565816456No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1380.10598834128245893No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA1380.10598834128245893No Hit
TACGGGGGGAGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGTGTA1360.10445227836532185No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1350.10368424690675329No Hit
TACGGAGGGGGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA1330.10214818398961623No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGCGAGTGCA1330.10214818398961623No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGCGTAAAGCGTGTGCA1320.10138015253104767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG152.0559168E-5227.000029
CGTATGA152.0559168E-5227.000023
AGGAGTC252.5356712E-9227.000026
GATCCAA152.0559168E-5227.000028
GAATCGA302.910383E-11227.000028
GGATCCA152.0559168E-5227.000027
CCGTCCA302.910383E-11227.000027
AGGTTGC152.0559168E-5227.000026
TACGAAC152.0559168E-5227.000021
ACAGAGA302.910383E-11227.000022
TAGGATG302.910383E-11227.000025
CACGTAA500.0227.000021
AATCGAG302.910383E-11227.000029
GTTGCAA500.0227.000028
ACGAACT152.0559168E-5227.000022
ATGGGGC750.0227.06
AGACGAG650.0227.09
TAGGATC1700.0227.05
AGGATCC2100.0227.06
TACAGAG2050.0227.01
Produced by FastQC (version 0.11.9)