FastQCFastQC Report
Mon 28 Nov 2022
4061-JW31trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW31trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35069
Sequences flagged as poor quality0
Sequence length233
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGTA424212.096153297784367No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA20145.742963871225299No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA18375.238244603495965No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA12383.530183352818729No Hit
GACGGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCCGCA11893.390458809775015No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA8092.3068807208645814No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA5851.6681399526647465No Hit
AACGAGGGGGACGAGCGTTATTCATCATGACTGGGCGTAAAAGGTTCGTA4651.3259573982719781No Hit
GACGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTA4041.1520145997889875No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA3671.0465083121845504No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTACGTA3601.0265476631783055No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3551.0122900567452737No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCATGCA3310.9438535458667199No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA3210.9153383330006558No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3030.8640109498417406No Hit
TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA2780.7927229176765804No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA1890.5389375231686104No Hit
TACGAAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA1650.4705010122900567No Hit
TACGAAGGGGGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA1580.4505403632838119No Hit
TACGAAGGGTGCAAGCGTTGCTCGGAATTATTGGGCGTAAAGGGTAGGTA1290.3678462459722262No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1240.35358863953919417No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGCGCACGTA1210.34503407567937494No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1200.3421825543927685No Hit
TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA1190.33933103310616214No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA1190.33933103310616214No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCTCGTA1120.3193703840999173No Hit
TACGGAGGGGGCAAGCGTTGTCCGGACTTACTGGGCGTAAAGGGTCCGTA1080.3079642989534917No Hit
TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1050.29940973509367247No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA1050.29940973509367247No Hit
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGGA1030.29370669252045967No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTCACTGGGCGTACAGGGTGTGTA1020.29085517123385324No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGCA950.2708945222276084No Hit
GACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA940.26804300094100203No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA800.22812170292851236No Hit
TACGGAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGTA760.21671561778208673No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA730.20816105392226755No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA720.20530953263566115No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA700.1996064900624483No Hit
TACGAAGGTGGCGAGCGTTGTTCGGATTCATTGGGCTTAAAGCGCACGTA680.19390344748923552No Hit
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGGGGGTA680.19390344748923552No Hit
TACGGGGGGGGCAAACGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGTA670.1910519262026291No Hit
TACGAAGGTCCCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGAGCGTA660.1882004049160227No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA660.1882004049160227No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA620.17679431976959709No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA610.17394279848299068No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA600.17109127719638426No Hit
TACGTAGGGGGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA550.15683367076335225No Hit
GACGGAGGAGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGCA530.15113062819013945No Hit
TACGAAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGTAAAGCGTGGGTA510.14542758561692662No Hit
TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA510.14542758561692662No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA490.13972454304371382No Hit
GACGAACCGAGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA490.13972454304371382No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTACAGGGTGCGTA470.13402150047050101No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA460.1311699791838946No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA450.12831845789728819No Hit
TACGGAGGGTCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA440.12546693661068178No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA440.12546693661068178No Hit
TACGTAGGGGCCAAGCGTTGTCCGGAATTACTGGGCGGAAAGCGTGGGTA430.1226154153240754No Hit
GACGAACCGTGCAAACGTTATTCGGAATCATTGGGCTTAAAGAGCGCGTA420.11976389403746898No Hit
TACGTAGGGGCCAAGCGTTGTCCGGACTTACTGGGCGTAAAGCGTGGGTA420.11976389403746898No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGGGCGTA410.11691237275086258No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA410.11691237275086258No Hit
TACGTATGGGGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGTCTA410.11691237275086258No Hit
GACAGAGGGGGCAAGCGTTGTCCGGAATTACTGGGCGTACAGCGCACGCA410.11691237275086258No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA400.11406085146425618No Hit
TACGTAGGGGGCGAGCGTTGTCCGAATTTACTGGGCGTAAAGCGCGCGTA400.11406085146425618No Hit
CACGTAAGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTACGTA390.11120933017764979No Hit
TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA390.11120933017764979No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA390.11120933017764979No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA380.10835780889104336No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA360.10265476631783058No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA360.10265476631783058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA100.0018474105227.000027
TAGGACG100.0018474105227.000025
ATCCAAG100.0018474105227.000029
GGCAAAG100.0018474105227.000029
TCGCGAG100.0018474105227.000029
ACGTAGT202.2535642E-7227.000022
GGATCCA100.0018474105227.000027
CACGAAC100.0018474105227.000021
CCGTACG100.0018474105227.000027
GATCCAA100.0018474105227.000028
CGTACGA100.0018474105227.000028
GTCCAAG202.2535642E-7227.000029
TACGAAG4750.0227.000021
AAGGTCC100.0018474105227.000025
AGATGGC100.0018474105227.000026
AGGTCCC202.2535642E-7227.000026
CGTGCAA100.0018474105227.000028
AAGGATC100.0018474105227.000025
TAGGGTC100.0018474105227.000025
GGTGCTA100.0018474105227.000028
Produced by FastQC (version 0.11.9)