FastQCFastQC Report
Mon 28 Nov 2022
4061-JW31trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW31trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35069
Sequences flagged as poor quality0
Sequence length232
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA586016.709914739513533No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATGTCGGCCCGGCG30028.560266902392426No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGT16064.579543186289885No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG10873.099603638541162No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA9022.572072200518977No Hit
CCTGTTTGCTACCCATACTTTCGTTCCTCAACGTCAGTTTTTACTAGAGA6391.8221221021414924No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT6171.7593886338361515No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG5561.5854458353531609No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGTGTCAGACATCGTCCAGAA4881.3915423878639255No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTACCGGGCCAGTG4781.3630271749978613No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG4561.3002937066925204No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG4351.240411759673786No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTCTAGTCCAGGG4341.2375602383871795No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG4091.1662722062220194No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG3951.1263509082095298No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGAGCCAGTG3390.9666657161595711No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG3350.9552596310131455No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG3350.9552596310131455No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC3340.952408109726539No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT3240.923892896860475No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGAACCGGTCCAGGT3220.9181898542872623No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG2730.7784653112435485No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT2670.76135618352391No Hit
CCTGTTTGCTCCCCACGCCATCGTGGCTCAGTGTCAGGTCTCGCCCAGAA2480.7071772790783883No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG2390.6815135874989307No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2300.655849895919473No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTCCGAGCCAGTG2100.5988194701873449No Hit
CCTGTTTGCTCCCCACGCTTTCGTAGTTGAGTGTCAGTATCTGTCCAGGT1860.5303829593087912No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG1740.4961647038695144No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG1710.4876101400096952No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC1700.4847586187230888No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGGCCAGGT1630.46479796971684395No Hit
CCGGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGATAAGGCCCAGGT1530.4362827568507799No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAATCGACGGCCAGGT1510.4305797142775671No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1500.42772819299096065No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATCGACCCAGGG1440.4106190652713223No Hit
CATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGT1370.3906584162650774No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTG1370.3906584162650774No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAATTTAGTA1230.35073711825258774No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG1210.34503407567937494No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGA1190.33933103310616214No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGGGTCAGTTATGGTCCAGT1160.33077646924634296No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTA1140.3250734266731301No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCAGTCCAGAT1140.3250734266731301No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG1120.3193703840999173No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGAAATGGGCCAGCA1040.2965582138070661No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT1040.2965582138070661No Hit
CCCGTTTGCTCCCCTGGCTTTCGCACCTCAGCGTCAGGATAGGCCTAGTG1030.29370669252045967No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG1000.28515212866064044No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTGTCGTCCAGGT990.28230060737403406No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG970.27659756480082126No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGGTACAGCCCAGAG950.2708945222276084No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGACCAGTT900.25663691579457637No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAGTT850.2423793093615444No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGCGTCAGTACCTGTCCAGGT840.23952778807493796No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC840.23952778807493796No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG830.23667626678833156No Hit
CCCTTTGGCTACCCACGCTTTCGTGCCTGAGCGTCAGCAATTGTCCAGGA730.20816105392226755No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGGA690.19675496877584192No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG680.19390344748923552No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGGTATGGACCAGAG680.19390344748923552No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT670.1910519262026291No Hit
CCCGTTCGCTACCCTAGCTTTCGCACCTCAGCGTCAGGATATGGCCAGGA670.1910519262026291No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGTAACTGTCCAGAG660.1882004049160227No Hit
CCCGTTCGCTACCCTAGCTTTCGCGCCTCAGCGTCAGAAGAGATCCAGCA650.1853488836294163No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGCATGTTCCAGAG620.17679431976959709No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATGATCCCAGGG620.17679431976959709No Hit
CCTGTTTGATCCCCACGCTTTCGCGCCTGAGCGTCAGTACCGAGCCAGAT610.17394279848299068No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA590.16823975590977785No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGGT570.16253671333656505No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA560.15968519204995865No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG550.15683367076335225No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGTCCAGTT530.15113062819013945No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGTGTCAGTTAAGCCCCAGTG530.15113062819013945No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATAACGGGCCAGAT520.14827910690353305No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG500.14257606433032022No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT500.14257606433032022No Hit
CCGGTTCGCTCCCCACGCTGTCGCACATGAGTGTCAGTTGCTGCCCAGGT450.12831845789728819No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCTTCAGCGTCAGTTCTAGGCCAGAG430.1226154153240754No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA430.1226154153240754No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATGAGCGTCAGAAATGCCCCAGCA420.11976389403746898No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGGGCCAGTG410.11691237275086258No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGA400.11406085146425618No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA400.11406085146425618No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG400.11406085146425618No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG400.11406085146425618No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTCTCGGGCCAGGA390.11120933017764979No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGAA390.11120933017764979No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTTGGCCCAGAT380.10835780889104336No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA380.10835780889104336No Hit
CCTGTTTGCTCCCCACGCCGTCGTGTCTCAGTGTCAGGTCTCGCCCAGAA360.10265476631783058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC4450.0226.000026
CCTGTTC4250.0226.000021
TTCGATC4400.0226.000025
CTGTTCG4250.0226.000022
GTTTGAT700.0226.04
TGTTCGC202.3037137E-7226.03
TGTTTGC22950.0226.03
TGTTTGA700.0226.03
GGTTCGC152.079466E-5226.03
CATTTGC750.0226.03
CCGTTCG1400.0226.02
CCCGTTC1400.0226.01
CCATTTG750.0226.02
TTGATCC700.0226.06
ATTTGCT750.0226.04
CTTCGAT400.0226.04
CTGTTTG23650.0226.02
CGGTTCG202.3037137E-7226.02
TTTGCTC19750.0226.05
TTTGCTA5750.0226.05
Produced by FastQC (version 0.11.9)