FastQCFastQC Report
Mon 28 Nov 2022
4061-JW32trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW32trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141490
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA7579853.57127712205809No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA20181.4262492048908049No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA12960.915965792635522No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA12080.8537705844936038No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA11920.8424623648314369No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10650.7527033712629868No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA10200.7208990034631423No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA8400.5936815322637642No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA8100.5724786203972012No Hit
TACAGAGGTGGCAAGCGTTGTTCGGATTCACTGGGTGTAAAGGTCATGTA8050.5689448017527741No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA7900.5583433458194925No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA7160.5060428298819705No Hit
CACCGGCGGCCCGAGTGGTAGCCGTTATTATTGGGTTTAAAGGGTCCGTA6800.4805993356420949No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA6770.47847904445543854No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA6690.47282493462435504No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA6210.4389002756378543No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA6030.4261785285179165No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA5470.38659975970033217No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA5290.3738780125803944No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4810.33995335359389356No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA4800.3392465898650081No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA4640.32793837020284117No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA4540.3208707329139868No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA4350.3074422220651636No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3990.281998727825288No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTCATTGGGCGTAAAGCGAATGTA3770.2664499257898085No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3560.25160788748321433No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA3320.23464555798996395No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA3250.22969821188776593No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA3210.22687115697222418No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA3150.2226305745989116No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA3080.21768322849671354No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2810.19860060781680686No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGCGAGTGCA2740.1936532617146088No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA2730.19294649798572336No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA2610.18446533323909817No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA2600.18375856951021274No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGCGCGTGCA2600.18375856951021274No Hit
CACCGGCGGCTCGAGTGGTAACCGTTATTATTGGGTCTAAAGGGTCTGTA2560.180931514594671No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA2550.18022475086578557No Hit
TACAGAGACCTCAAGCGTTATCCGGATTAATTGGGCGTAAAGCGCAGGTA2430.17174358611916038No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA2410.17033005866138948No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA2380.1682097674747332No Hit
CACGTAGGAATCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA2130.15054067425259735No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA2120.14983391052371192No Hit
TACCGGCGGCTCGAGTGGTGGCCACTATTACTGGGCTTAAAGCGTTCGTA2040.14417980069262845No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2020.14276627323485758No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1970.1392324545904304No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1940.13711216340377413No Hit
AACGTAGGAAGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA1910.1349918722171178No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA1860.13145805357269064No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1830.12933776238603434No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1790.12651070747049262No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA1720.12156336136829458No Hit
CACCGGCGGCCCGAGTGGTGATCGTGATTATTGGGTCTAAAGGGTCCGTA1660.11732277899498197No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1650.11661601526609655No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1640.11590925153721111No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1610.1137889603505548No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA1610.1137889603505548No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1600.11308219662166938No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA1590.11237543289278394No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGCGCGTGCA1570.11096190543501308No Hit
TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA1560.11025514170612763No Hit
AACGTAGGAGACGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1540.10884161424835677No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA1470.10389426814615874No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1420.10036044950173158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC202.2777567E-7227.000025
GGAATCG100.0018533366227.000027
CGTATGA152.0562911E-5227.000023
TACCAGC152.0562911E-5227.000021
GATCCAA202.2777567E-7227.000028
GGTCCCG152.0562911E-5227.000027
CTCTTCA100.0018533366227.000027
CACGAAC152.0562911E-5227.000021
CCGTCCA152.0562911E-5227.000027
ACCTCAA202.2777567E-7227.000028
GCACCAA100.0018533366227.000028
GCTCTCC100.0018533366227.000026
ATGAGGC152.0562911E-5227.000026
GTATGAG152.0562911E-5227.000024
GAGCGAG152.0562911E-5227.000029
CCTCAAG202.2777567E-7227.000029
ACGGAGT100.0018533366227.000022
ACCAGCT302.910383E-11227.000022
AGACCCC100.0018533366227.000026
GGACGCA202.2777567E-7227.000027
Produced by FastQC (version 0.11.9)