FastQCFastQC Report
Mon 28 Nov 2022
4061-JW34trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW34trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136217
Sequences flagged as poor quality0
Sequence length233
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA5101537.451272601804476No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA70195.15280765249565No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA33372.4497676501464576No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA26371.9358817181409076No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA25561.8764177745802653No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA24591.8052078668594964No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA20621.5137611311363486No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA19901.4609042924157778No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA18371.3485835101345647No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA10720.7869795987284994No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA9640.7076943406476431No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA9030.6629128522871595No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA8880.6519010108870406No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA8500.6240043460067393No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA7030.5160883002855737No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA6300.462497338804995No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA5890.4323983056446699No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA5190.3810097124441149No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5020.3685296255239801No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4910.3604542751638929No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA4870.3575177841238612No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA4760.349442433763774No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA4550.3340258558036075No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA4460.3274187509635361No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA3930.28851024468311587No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3730.27382778948295733No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA3640.267220684642886No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3620.26575243912287017No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA3620.26575243912287017No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA3590.2635500708428463No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA3500.256942966002775No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA3500.256942966002775No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3480.2554747204827591No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA3460.2540064749627433No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3340.24519700184264812No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA3280.2407922652826006No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA3260.23932401976258472No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA3030.22243919628240233No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2850.2092249866022596No Hit
GACAGAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGCGAACGCA2850.2092249866022596No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA2850.2092249866022596No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2840.2084908638422517No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA2810.20628849556222792No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2770.2033520045221962No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2730.2004155134821645No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA2680.19674489968212486No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA2670.1960107769221169No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2660.195276654162109No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2640.19380840864209312No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA2570.18866954932203764No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA2570.18866954932203764No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGTGTA2550.18720130380202177No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA2510.18426481276199005No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA2450.17986007620194247No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA2410.17692358516191078No Hit
TACGGGGGGAGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGTGTA2400.17618946240190284No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2320.1703164803218394No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2070.1519634113216412No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA2060.15122928856163328No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2050.15049516580162536No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA2030.1490269202816095No Hit
TACAGGGGTGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA1950.14315393820154607No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGCAAGCA1950.14315393820154607No Hit
CACCGGCGGCTCGAGTGGTAACCGTTATTATTGGGTCTAAAGGGTCTGTA1880.13801507888149056No Hit
CACGTAAGGGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGTACGTA1870.13728095612148264No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA1870.13728095612148264No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGCGTAAAGCGTGTGCA1860.13654683336147472No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGAGCA1830.13434446508145093No Hit
GACAGAGGGTGCGAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1820.133610342321443No Hit
CACGTAGGAGTCTAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGTA1810.13287621956143508No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA1760.12920560576139542No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGTGTGCA1740.12773736024137955No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA1720.1262691147213637No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA1710.12553499196135579No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTCATTGGGCGTAAAGCGAATGTA1640.1203961326413003No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1640.1203961326413003No Hit
AACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA1620.11892788712128442No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1610.1181937643612765No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1600.11745964160126857No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1600.11745964160126857No Hit
TACGTAGGGGGTAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCATGTA1580.1159913960812527No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1540.113054905041221No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGCGAGTGCA1520.11158665952120514No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1520.11158665952120514No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA1500.11011841400118928No Hit
TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA1450.10644780020114965No Hit
TACAGAGACCTCAAGCGTTATCCGGATTAATTGGGCGTAAAGCGCAGGTA1420.10424543192112586No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA1420.10424543192112586No Hit
AACGTAGGAAGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA1380.10130894088109413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG8650.0226.997451
AGCACCC202.277593E-7226.997445
ATGGGGC500.0226.997446
GACAGGG100.0018533241226.997441
TACCCGC100.0018533241226.997441
GGAGCCT100.0018533241226.997447
CGTATGA100.0018533241226.997443
TACCAGC252.5356712E-9226.997441
ACGCAAG350.0226.997449
AGCCAAG3900.0226.997449
CTCTTCA100.0018533241226.997447
AGTGGCA152.0562169E-5226.997447
TACGGAT100.0018533241226.997441
ACCTCAA202.277593E-7226.997448
CCGTACG302.910383E-11226.997447
TACGAAG2000.0226.997441
ACGTGGG100.0018533241226.997442
ACCAGCT152.0562169E-5226.997442
ACCAGCA202.277593E-7226.997442
ACGTATG750.0226.997442
Produced by FastQC (version 0.11.9)