FastQCFastQC Report
Mon 28 Nov 2022
4061-JW34trimY_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW34trimY_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136217
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA4026329.557984686199223No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA84026.168099429586615No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT73925.426635441978608No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA53393.9194814156823305No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG34062.5004221205870047No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG27522.0203058355418193No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA27241.9997503982615974No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA23861.7516169053789177No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT21481.5768956884970307No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA8820.647496274326993No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT8400.6166631184066599No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA7730.5674768934861287No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG7690.564540402446097No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC7370.5410484741258433No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA6870.5043423361254469No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA6480.4757115484851377No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA5990.4397395332447491No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT5340.3920215538442338No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA5340.3920215538442338No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC5180.38027558968410696No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA5020.3685296255239801No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA4990.3663272572439563No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA4960.3641248889639325No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC4860.35678366136385326No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA4810.3531130475638136No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG4680.34356945168371056No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG4640.3406329606436788No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA4510.33108936476357576No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT4470.328152873723544No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA4410.3237481371634965No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA3750.27529603500297317No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA3600.26428419360285427No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3520.25841121152279084No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3390.24886761564268775No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT3320.24372875632263225No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG3090.2268439328424499No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT3080.226109810082442No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG2980.2187685824823627No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2900.2128956004022993No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG2860.20995910936226755No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA2860.20995910936226755No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG2770.2033520045221962No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA2680.19674489968212486No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2650.19454253140210107No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATCACGGCCCGGCG2570.18866954932203764No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA2560.1879354265620297No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTGCGCCAGTG2560.1879354265620297No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA2540.18646718104201385No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2520.184998935521998No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA2510.18426481276199005No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA2470.18132832172195834No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT2410.17692358516191078No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT2360.17325297136187112No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA2330.17105060308184736No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA2260.16591174376179185No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2250.16517762100178393No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG2220.16297525272176014No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG2200.16150700720174427No Hit
CCCATTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAATGGTCCAGGA2100.15416577960166497No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA2020.14829279752160154No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGCTTAGCA2010.14755867476159362No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG1980.14535630648156986No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTCAGCGTCAGGTACGGTCCAGGA1940.14241981544153814No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA1860.13654683336147472No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG1820.133610342321443No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCAGTCCAGGA1790.1314079740414192No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA1740.12773736024137955No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGGA1720.1262691147213637No Hit
CCCGTTCGCTACCCATGCTTTCGCGTCTTAGCGTCAGGTACAGCCCAGGG1700.12480086920134785No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA1680.123332623681332No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT1660.12186437816131612No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTGTCGGTCCAGGA1640.1203961326413003No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGTTTAGTA1640.1203961326413003No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT1640.1203961326413003No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACGGGCCCAGCG1590.11672551884126063No Hit
CCTGTTCGCTCCCCACGCTTTCGCATATCAGCGTCAATGTTAGGCCAGAG1570.11525727332124477No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAGTGGCCCAGGG1560.11452315056123684No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1540.113054905041221No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1530.11232078228121307No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA1520.11158665952120514No Hit
CCCGTTCGCTACCCTAGCTTTCGAGTCTGAGTGTCAGGATCAGACCAGGG1510.1108525367611972No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA1510.1108525367611972No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCGTTCCAGAA1480.10865016848117341No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGATGCAGTCCAGAA1470.10791604572116548No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTATAGCTTAGCT1470.10791604572116548No Hit
CCCGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGCTGGGCCAGTG1420.10424543192112586No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGAACCCGTTCTGGTA1420.10424543192112586No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC1400.10277718640110999No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1380.10130894088109413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCT100.0018759713226.078234
GTTTGCG100.0018759713226.078234
GGTTGCT100.0018759713226.078234
TGTTCGC2750.0226.078233
CCGTTCG10750.0226.078232
GCTTGCT152.0897716E-5226.078234
CTGTTCG6900.0226.078232
TTTGCTG100.0018759713226.078235
TTTGCTA11800.0226.078235
TGTGCTC100.0018759713226.078235
TGTGTGC100.0018759713226.078233
GTTGCTC100.0018759713226.078235
TTGCTAC11900.0226.078236
GTGCTCC100.0018759713226.078236
GTTTGAT3950.0226.078224
TTCGATC4200.0226.078225
TGTTTGA3950.0226.078223
GGTTCGC9150.0226.078223
CCCGTTT8350.0226.078221
CGGTTTG650.0226.078222
Produced by FastQC (version 0.11.9)