FastQCFastQC Report
Mon 28 Nov 2022
4061-JW36trimY_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW36trimY_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119093
Sequences flagged as poor quality0
Sequence length233
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA4436837.25491842509635No Hit
TACGTAGGATGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGAGTA38943.269713585181329No Hit
TACGGAGGGGGCAAGCGTTACTCGGAATTATTGGGCGTAAAGGGCGCGTA21031.7658468591772816No Hit
GACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCTTAAAGGGCGCGTA20401.7129470245942247No Hit
CACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA18801.5785982383515405No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA16351.37287665941743No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGTGTA16221.3619608205352118No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA14711.2351691535186786No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA14331.2032613167860413No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA13281.1150949258142795No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGCACGTA12911.0840267689956589No Hit
GACGGAGGGGGCAAGCGTTGTCCGGACTCACTGGGCGTAAAGCGTACGTA11680.9807461395715953No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGTGTA9270.7783832802935521No Hit
CACGTAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA8560.718766006398361No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA8430.7078501675161428No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGATGCGTA7900.6633471320732537No Hit
TACGTAGGGGGCTAGCGTTACTCGGATTTATTGGGCGTAAAGCGAATGTA7670.6440344940508679No Hit
CACGTAGGAGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGCGCGTGCA7000.5877759398117437No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGCGTGCA6240.5239602663464688No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5560.4668620321933279No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA5460.4584652330531601No Hit
CACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCATGTA5400.45342715356905944No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA5390.45258747365504265No Hit
TACGGAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGCA5010.4206796369224052No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA4630.38877180018976765No Hit
GACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGTGTA3970.33335292586466037No Hit
TACAGGGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA3870.3249561267244926No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAACGCGTA3700.31068156818620746No Hit
TACGGGGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3660.3073228485301403No Hit
TACGGAGGACCCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA3520.2955673297339054No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA3520.2955673297339054No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGCA3320.2787737314535699No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA3170.26617853274331826No Hit
TACGTATGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA3150.2644991729152847No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGGGCGCA3120.2619801331732344No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGGGTAAAGGGTGCGCA3070.2577817336031505No Hit
TACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA2960.24854525454896595No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA2940.2468658947209324No Hit
TACGGAGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA2860.24014845540879817No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGCA2660.22335485712846265No Hit
GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2470.20740093876214388No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA2410.2023628592780432No Hit
TACGTAGGGGGCAAGCGTTACTCGGATTTATTGGGCGTAAAGAGAATTAA2400.20152317936402644No Hit
CACCGGCGGCTCGAGTGGTAACCGTTATTATTGGGTCTAAAGGGTCTGTA2340.1964850998799258No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGAGCGCGTA2340.1964850998799258No Hit
CACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGTA2290.19228670030984188No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA2240.188088300739758No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA2230.18724862082574123No Hit
AACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGCGTGTGCA2200.1847295810836909No Hit
CACGTAGGAGACGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGTGCA2180.18305022125565734No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGTA2140.17969150159959024No Hit
AACGTAGGAGGCAAGCGTTATCCGAATTTACTGGGTGTAAAGCGCGTGCA2110.1771724618575399No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGCA2100.17633278194352314No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA2010.16877566271737215No Hit
TACGGGGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGAGCGTGTA2000.16793598280335537No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1940.16289790331925472No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAATGCGTA1890.15869950374917083No Hit
GACGGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA1710.14358526529686882No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGTGTA1700.14274558538285204No Hit
GACAGAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGCGAACGCA1670.14022654564080173No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1640.13770750589875141No Hit
TACGGGGGGTGCAAGCGTTGTTCGGATTTATTGGGCGTAAAGCGCGTGTA1640.13770750589875141No Hit
CACGTAGGAATCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGCGTGCA1620.13602814607071784No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA1600.1343487862426843No Hit
TACGTAGGATCCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGCGTGTA1580.13266942641465074No Hit
TACAGGGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGCA1570.13182974650063395No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA1530.12847102684456685No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA1520.1276313469305501No Hit
AACGTAGGAGCCAAGCGTTATCCGAATTCACTGGGCGTAAAGCGTGTGCA1510.1267916670165333No Hit
CACGTAGGATCCGAGCGTTATCCGAATTTACTGGGCGTAAAGCGTGTGCA1480.12427262727448297No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA1440.12091390761841585No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA1440.12091390761841585No Hit
AACGTAGGAGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTGCA1410.11839486787636552No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTAGGTTTAAAGGGTGCGCA1410.11839486787636552No Hit
AACGTAGGAGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGCGCGAGCA1400.11755518796234875No Hit
CACCGGCGGCCCGAGTGGTGATCGTGATTATTGGGTCTAAAGGGTCCGTA1390.11671550804833197No Hit
TACCGGCGGCTCGAGTGGTGGCCACTATTACTGGGCTTAAAGCGTTCGTA1380.11587582813431521No Hit
AACGTAGGAAGCGAGCGTTATCCGGATTTACTGGGCGTAAAGCGTGTGTA1360.11419646830628165No Hit
TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA1350.11335678839226486No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1330.11167742856423132No Hit
GACGGAGGGTGCTAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA1310.10999806873619776No Hit
TACAGGGGAGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGCA1250.1049599892520971No Hit
TACAGAGGTGTCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA1210.10160126959603No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCACGTGTA1200.10076158968201322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGC100.001852969227.000026
CGAACCA100.001852969227.000023
CACGGGG100.001852969227.000021
GACGTAG550.0227.000021
AGGACCC202.2762651E-7227.000026
GGATCCA100.001852969227.000027
GCGCCTC100.001852969227.000026
ATGCTAG100.001852969227.000029
TACATAG202.2762651E-7227.000021
TGTTGCA100.001852969227.000027
CCGTACG100.001852969227.000027
GAGACAA100.001852969227.000028
CATAGGG202.2762651E-7227.000023
CGGATGC100.001852969227.000026
AGACAAG100.001852969227.000029
CGTACGA100.001852969227.000028
ACAGAGA100.001852969227.000022
ACCGTCC100.001852969227.000026
GCAGCAA202.2762651E-7227.000028
CACGTAA252.5338522E-9227.000021
Produced by FastQC (version 0.11.9)