FastQCFastQC Report
Wed 30 Nov 2022
4061-JW01trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW01trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90422
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG1815920.082502045962265No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA1663518.397071509146006No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA87679.695649288889872No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA21642.393222888235164No Hit
TAAGGAATATTGCGCAATGGGCGACAGCCTGACGCAGCGACGCCGCGTGG12211.3503350954413749No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA10621.1744929331357412No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG9000.9953329941828315No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA8580.9488841211209662No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAAGACCGCGTGG7110.786313065404437No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGA6010.6646612550043132No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC5950.6580257017097609No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC5820.6436486695715645No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAAGACCGCGTGA5760.6370131162770122No Hit
TTTCGAATCATTCACAATGGGCGAAAGCCTGATGGTGCGACGCCGCGTGA5170.5717635088805821No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA5130.567339806684214No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC5100.564022030036938No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA5060.5595983278405697No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGC4990.5518568489969256No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG4800.5308442635641768No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGA3790.4191457831058813No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA3650.4036628254185928No Hit
TAAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATCCCGCGTGC3600.39813319767313265No Hit
TGAGGAATATTGCTCAATGGGCGCAAGCCTGAAGCAGCGACGCCGCGTGG3590.39702727212404065No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAATGCCGCGTGC3560.3937094954767645No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGG3440.3804383888876601No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT3230.35721395235672737No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA3180.3516843246112672No Hit
TGGGGAATCTTGGTCAATGGGCGCAAGCCTGAACCAGCGACGCCGCGTGA3050.33730729247307073No Hit
TCGAGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACGCCGCGTGG2980.3295658136294265No Hit
TAACGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGT2920.32293026033487426No Hit
TGAGGAATATTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGG2880.3185065581385061No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAACGCCGCGTGG2740.3030236004512176No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA2730.30191767490212557No Hit
TCGAGAATTTTTCTCAATGGGCGAAAGCCTGAAGGAGCGACGCCGCGTGG2650.2930702705093893No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA2640.2919643449602973No Hit
TGGGGAATCTTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGG2630.29085841941120527No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG2600.2875406427639291No Hit
TGAGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA2460.27205768507664063No Hit
TCGAGAATCATTCGCAATGGGCGAAAGCCTGACGGTGCGACGCCGCGTGG2370.26210435513481234No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG2360.2609984295857203No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC2350.2598925040366283No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA2320.2565747273893521No Hit
TCGAGAATCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGC2200.24330362080024773No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGT2170.23998584415297164No Hit
TAAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG2110.23335029085841943No Hit
TGGGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGT2060.22782066311295923No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGGGGG1940.21454955652385482No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGT1900.21012585432748668No Hit
TAGGGAATATTGGACAATGGGTGCGAGCCTGATCCAGCCATGCCGCGTGC1820.2012784499347504No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA1810.20017252438565836No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGAGGG1710.189113268894738No Hit
CAAGGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGG1690.18690141779655395No Hit
TCGAGGATCTTCGGCAATGGGCGCAAGCCTGACCGAGCGACGCCGCGTGT1660.18358364114927783No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGT1660.18358364114927783No Hit
TCGAGAATCTTCGGCAATGGGCGCAAGCCTGACCGAGCGACGCCGCGTGC1630.18026586450200172No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1560.17252438565835748No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG1500.16588883236380525No Hit
TGGGGAATATTGGACAATGGGGGAAACCCTGATCCAGCGACGCCGCGTGT1460.16146513016743713No Hit
TCGAGGATCTTCGGCAATGGGGGCAACCCCGACCGAGCGATGCCGCGTGG1450.1603592046183451No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCCATGCCGCGTGG1440.15925327906925305No Hit
TCGAGAATAATTCACAATGGGCGAAAGCCTGATGGTGCAACGCCGCGTGG1420.157041427971069No Hit
TGGGGAATTTTGGGCAATGGCCGAAAGGCTGACCCAGCAACGCCGCGTGA1370.1515118002256088No Hit
TCGAGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGC1320.14598217248014864No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT1310.1448762469310566No Hit
TCGAGAATCTTTCTCAATGGGGGAAACCCTGAAGGAGCGACGCCGCGTGG1290.14266439583287255No Hit
TAACGAATCTTCCACAATGCACGAAAGTGTGATGGAGCGACGCCGCGTGT1250.1382406936365044No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGA1210.13381699144013626No Hit
TAAGGAATATTGGACAATGGGCGGAAGCCTGATCCAGCCATGCCGCGTGC1200.1327110658910442No Hit
TCGAGGATCTTCGGCAATGCGCGCAAGCGCGACCGAGCGACGCCGCGTGG1200.1327110658910442No Hit
TCGAGAATCTTCGGCAATGGACGCAAGTCTGACCGAGCGACGCCGCGTGC1180.13049921479286014No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCACGCCGCGTGA1140.126075512596492No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGT1050.11612218265466369No Hit
TGGGGAATCTTGGTCAATGGGCGCAAGCCTGAACCAGCGATGCCGCGTGA1050.11612218265466369No Hit
TGGGGAATCTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGG1040.11501625710557166No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGG1030.11391033155647962No Hit
TAAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGG1010.11169848045829556No Hit
TCGAGAATTTTTCTCAATGGGCGCAAGCCTGAAGGAGCGACGCCGCGTGG990.10948662936011147No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCACGCCGCGTGA960.10616885271283537No Hit
TGGGGAATTTTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA960.10616885271283537No Hit
TCGAGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG950.10506292716374334No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCCACGCCGCGTGA940.1039570016146513No Hit
TAGGGAATATTGGTCAATGGATGGAAGTCTGAACCAGCCATGCCGCGTGC940.1039570016146513No Hit
TCGAGAATTTTTCTCAATGGACGAAAGTCTGAAGGAGCGACGCCGCGTGG930.10285107606555927No Hit
TCGGGAATTTTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA920.10174515051646724No Hit
TAAGGAATATTGGTCAATGGACGAAAGTCTGAACCAGCCATGCCGCGTGG910.1006392249673752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCT450.0228.000028
CGAGGAT900.0228.000022
ATTGCTC450.0228.000029
ATATTCC450.0228.000027
TATATTG100.001828022228.06
TAGGAGC252.4629117E-9228.03
GATATTG152.018142E-5228.06
AATTTTG3650.0228.06
AATTTTA100.001828022228.06
TTTCCAC202.2239692E-7228.09
GCGAATC100.001828022228.03
TGTTCGC650.0228.09
GAGCTTC252.4629117E-9228.06
TTTTCAC202.2239692E-7228.09
GTCGAGA100.001828022228.03
GTTTCCG100.001828022228.08
TATTGCG3650.0228.08
GTATATT100.001828022228.05
TTTTACG100.001828022228.08
GAGTATT100.001828022228.05
Produced by FastQC (version 0.11.9)