FastQCFastQC Report
Wed 30 Nov 2022
4061-JW01trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW01trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90422
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC1299114.367078808254629No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA75138.30881865032846No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTAATGGCCCAGCA49495.473225542456482No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTGATGGCCCAGCA47795.285218199110836No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGATAAGGTCCAGCA47285.228815996107142No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATAGCCCAGCA17681.955276370794718No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT16361.8092941983145694No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG11731.2972506690849572No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTCTGGTCCAGTA9971.1026077724447592No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA8710.9632611532591626No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA6600.7299108624007432No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG6130.6779323615934175No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA5290.5850346154696866No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG5240.5795049877242264No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA5190.5739753599787663No Hit
CCCTTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAATTGTCCAGGA4790.5297383380150849No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGGGTCAGTTGTGGTCCAGC4710.5208909336223485No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG4340.4799716883059433No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTTCGGCCCAGAG3970.43905244298953794No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAAAGGCCCAGTT3490.38596801663312025No Hit
CCTGTTTGCTACCCACGCTATCGTCCCTCAGCGTCAATCATCGTCTAGCC3220.3561080268076353No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCGCTGTCCAGAA3060.33841321802216273No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGACAGGCCCAGCC3050.33730729247307073No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAATACTTGTCCAGTG3020.3339895158257946No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT3000.3317776647276105No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG2950.3262480369821504No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTGTGGACCAGAG2850.31518878149123003No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA2850.31518878149123003No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG2800.3096591537457698No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA2750.3041295260003097No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC2590.2864347172148371No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGATGGGGTCCAGCG2590.2864347172148371No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGGG2440.2698458339784566No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG2420.2676339828802725No Hit
CCTGTTTGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTGTTCGTCCAGTT2290.25325695074207605No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAAAAATTGCCCAGTT2280.252151025192984No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA2240.24772732299661587No Hit
CCCGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGGCCAAGGCCAGGG2210.24440954634933976No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTCAGCGTCAGGTTGGTCCCAGGG2050.22671473756386717No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGT2020.22339696091659111No Hit
CCCGTTTGCTACCTTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTA2000.22118510981840703No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG1990.22007918426931497No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTATCGTCCAGAA1900.21012585432748668No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACCGTCCAGAA1860.20570215213111853No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTTCTGGACCAGGA1840.20349030103293447No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA1770.19574882218929024No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1690.18690141779655395No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG1660.18358364114927783No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1600.1769480878547256No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA1560.17252438565835748No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA1510.1669947579128973No Hit
CCCTTTTGCTCCCCTGGCCTTCGAGCCTCAGCGTCAGATGTTGTCCAGTA1480.1636769812656212No Hit
CCTTTTCGCTCCCCTGGCCTTCGTCTCTCAGCGTCAGGTGTCGTCCAGAG1420.157041427971069No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCTAGGTCCAGCA1350.14929994912742475No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA1330.14708809802924067No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1310.1448762469310566No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGATCTGTCCAGGG1250.1382406936365044No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT1210.13381699144013626No Hit
CCTGTTTGCTCCCCACGCTTTCGACCATGAGCGTCAGTATCGGGCCAGAG1200.1327110658910442No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA1190.1316051403419522No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGATTCAGTGTCAGGAATAGTCCAGAG1170.1293932892437681No Hit
CCCGTTTGCTACCCTAGCTTTCGTGCATCAGCGTCAGAAGAGATCCAGAA1110.12275773594921591No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCAGGTCAAGCCCAGGA1100.12165181040012386No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA1070.11833403375284776No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGTT1060.11722810820375572No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTGATGGCCCAGCA1050.11612218265466369No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG1020.11280440600738759No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCGATCTCGGTCCAGGA1020.11280440600738759No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCT1000.11059255490920351No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG980.10838070381101944No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGCCCAGC960.10616885271283537No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTTAGCGTCAGTACCGGTCCAGAT950.10506292716374334No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT950.10506292716374334No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA950.10506292716374334No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATTGTCCAGAG940.1039570016146513No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA940.1039570016146513No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTTACAGTCTAGTA930.10285107606555927No Hit
CCTTTTCGCTCCCCTGACCTTCGTCTCTCAGCGTCAGTAATTGTCCAGAG930.10285107606555927No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG920.10174515051646724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC22750.0226.000023
TGTTTGA2500.0226.000023
GTTTGAT2500.0226.000024
CGTTTGC5000.0226.000023
ATTTGCT10850.0226.000024
GTTTGCT27850.0226.04
CGGTTCG202.3238317E-7226.02
GGATCGC252.5975169E-9226.03
TCGATCC152.0903404E-5226.06
ATCCGCT100.001876875226.04
CCTTTTT100.001876875226.01
CTTGCTC152.0903404E-5226.05
CGGATCG252.5975169E-9226.02
TATTCGC100.001876875226.03
TGTTCGA152.0903404E-5226.03
ATCCCCG100.001876875226.09
CTGTTTG25250.0226.02
TTTGCTG100.001876875226.05
CGTTTTG100.001876875226.02
GCTCCCA100.001876875226.08
Produced by FastQC (version 0.11.9)