FastQCFastQC Report
Wed 30 Nov 2022
4061-JW02trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW02trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110176
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA4500440.84737147836189No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA82747.509802497821667No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA44634.050791460935231No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA14281.2961080453093232No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC13121.1908219575951204No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT9970.9049157711298286No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA8780.7969067673540516No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT8280.7515248329944816No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC7320.6643915190241069No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA6260.5681818181818182No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT6130.5563825152483299No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA5380.48830961370897474No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA4990.45291170490851007No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA4420.40117629973860003No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG3670.33310339819924484No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG3170.2877214638396747No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA3090.28046035434214345No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA2830.256861748475167No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA2820.2559541097879756No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG2720.24687772291606158No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2530.22963258785942492No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2420.21964856230031948No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA2400.21783328492593668No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA2390.21692564623874527No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA2260.20512634330525706No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA2030.18425065349985476No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA2010.18243537612547198No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1970.17880482137670636No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1930.17517426662794075No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG1880.17063607319198373No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC1820.16519024106883531No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA1810.1642826023816439No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT1780.1615596863200697No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1780.1615596863200697No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA1690.1533909381353471No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTTCGGTCCAGGA1660.15066802207377286No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1600.14522218995062444No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1600.14522218995062444No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG1590.14431455126343304No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1580.14340691257624166No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA1560.14159163520185886No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA1520.13796108045309324No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA1520.13796108045309324No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA1490.13523816439151903No Hit
CCTGTTTGCTCCCCTTGCTTTCGTGCCTCAGCGTCAGGAGTAGGCTAGAA1490.13523816439151903No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG1490.13523816439151903No Hit
CCCGTTTGCTACCCTAGCTGTCGCGTCTGAGCGTCAGAAATGGTCCAGAA1480.1343305257043276No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG1460.13251524832994482No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCAGTCCAGGA1440.13069997095556202No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1410.1279770548939878No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1340.12162358408364798No Hit
CCCATTCGCTACCCTAGCTTTCGCGTCTTAGCGTCAGAAACGGTCCAGAA1280.11617775196049956No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA1220.11073191983735114No Hit
CCTGTTTGCTCCCCACGCTTTCGTATCTTAGCGTCAGAAACTGTCTAGTG1220.11073191983735114No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT1210.10982428115015974No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACAGTGCCAGAG1180.10710136508858555No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1170.10619372640139413No Hit
CCCGTTTGCTACCTTAGCTTTCGTGTCTGAGCGTCAGAAATGGGCCAGGA1170.10619372640139413No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1150.10437844902701132No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1120.10165553296543713No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1110.10074789427824571No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG1110.10074789427824571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA1900.0226.090843
TTGATAC152.0882246E-5226.090846
GTTTGCT85850.0226.090844
TTGCTGC152.0882246E-5226.090846
GTTCGAT1850.0226.090844
GTTCACT252.593879E-9226.090844
TTTGATA152.0882246E-5226.090845
TGTGTGC252.593879E-9226.090843
TGTTTGC79600.0226.090823
TTGCTAC6950.0226.090826
TGTGCTC202.321467E-7226.090825
TGGTTGC202.321467E-7226.090823
GTGTGCT202.321467E-7226.090824
GTTCGTG350.0226.090824
CCTTTTG100.0018751723226.090822
CTGTTTG81450.0226.090822
CTTTGCT350.0226.090824
TTTGCTG100.0018751723226.090825
TTTGCTC80300.0226.090825
TTTGCTA6950.0226.090825
Produced by FastQC (version 0.11.9)