FastQCFastQC Report
Wed 30 Nov 2022
4061-JW03trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW03trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146433
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA6536444.63747925672492No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA52163.562038611515164No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA44883.064882915736207No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT28031.9141860099840882No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA21291.4539072476832409No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC18591.2695225802926935No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA14470.988165235978229No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG13820.9437763345693936No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG13000.8877780281767089No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA9670.6603702717283672No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT9230.6303224000054632No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA9110.6221275258992167No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC7190.491009540199272No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT6850.4677907302315735No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA6090.415889860892012No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC6040.4124753300144093No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG5430.37081805330765605No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC5370.3667206162545328No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGTGTCAGTTCAGACCCAGAT5220.3564770236217246No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA5010.34213599393579314No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT4900.33462402600506713No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT4580.31277102838840976No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA4460.30457615428216317No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA4380.2991129048779988No Hit
CACTGACGGATTTTACCGTCCACGACTCTCTGAACTCCTCAAACCAGGAA4360.29774709252695775No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG4060.27725990726134137No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA3380.23082228732594431No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA3260.22262741321969776No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT3250.22194450704417723No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA3190.21784706999105394No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3130.21374963293793064No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG3020.20623766500720464No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2980.20350604030512248No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA2740.1871162920926294No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2730.18643338591710887No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA2650.1809701365129445No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA2610.1782385118108623No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA2600.17755560563534176No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG2590.1768726994598212No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA2430.1659462006514925No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA2400.16389748212493085No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC2370.16184876359836922No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG2270.15501970184316377No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2230.15228807714108158No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2150.1468248277369172No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCGTTCCAGAA2130.14545901538587613No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA2120.14477610921035558No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2110.14409320303483503No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA2090.14272739068379395No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT2010.13726414127962958No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA1960.13384961040202686No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGCTTAGCA1940.13248379805098576No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT1880.1283863609978625No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA1870.12770345482234197No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA1810.12360601776921869No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1800.12292311159369815No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA1770.1208743930671365No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1750.11950858071609542No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA1750.11950858071609542No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA1720.11745986218953378No Hit
CCCGTTTGCTCCCCTAGCTTTCGCATCTTAGCGTCAGGAACGGCCCAGGA1630.11131370660984888No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA1590.10858208190776669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACT152.0929792E-5226.000024
GCTTGCT600.0226.000024
TCGCTGC152.0929792E-5226.000026
CGTTTTG152.0929792E-5226.000022
TATTTGC100.0018780595226.03
TGTTTGA1500.0226.03
GTTTGCG100.0018780595226.04
TTTACTA100.0018780595226.05
CTTTTTG252.6047928E-9226.02
TTGCTTC202.3287248E-7226.06
CTCTTTG100.0018780595226.02
TGTGCTC202.3287248E-7226.05
TTTTTGC252.6047928E-9226.03
ATCCGCA100.0018780595226.09
TGTTCGA3000.0226.03
CTGTTGG100.0018780595226.02
GACGGAT400.0226.05
TTTGATC1500.0226.05
GGTTCGC2550.0226.03
TGTGTGC252.6047928E-9226.03
Produced by FastQC (version 0.11.9)