FastQCFastQC Report
Wed 30 Nov 2022
4061-JW06trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW06trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142806
Sequences flagged as poor quality0
Sequence length234
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG7121049.864851616878845No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC110427.732168116185595No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG88706.211223618055263No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG26651.866168088175567No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC23151.6210803467641415No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC17641.2352422167135835No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG10450.7317619707855413No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG10020.7016511911264233No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG8260.5784070697309637No Hit
GGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGGGTG8020.5616010531770373No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG7160.5013794938588014No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG6740.47196896488943035No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4800.33612033107852607No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA4460.3123118076271305No Hit
TCGAGAATATTCCACAATGGACGAATGTCTGATGGAGCGACGCCGCGTGC4250.297606543142445No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA4030.28220102796801255No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC3950.27659902245003715No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG3790.2653950114140863No Hit
CGAGGAATTTTCCGCAATGGACGAATGTCTGACGGAGCAACGCCGCGTGG3640.2548912510678823No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA3580.2506897469294007No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGT3520.24648824279091913No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA3500.2450877414114253No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA3440.2408862372729437No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG3380.23668473313446214No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA3350.23458398106522135No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG3340.23388373037547444No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA3190.22337997002927049No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG3000.2100752069240788No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2950.20657395347534416No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG2880.20167219864711564No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC2840.19887119588812796No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC2840.19887119588812796No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA2790.1953699424393933No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG2690.188367435541924No Hit
TAGGGAATATTAGTTAATGTGCGAAAGCGCGAACTAGCAACGCCGCGTGT2580.18066467795470778No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC2560.17926417657521393No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT2470.17296192036749156No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGA2400.16806016553926303No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2320.16245816002128763No Hit
CTAGGAATTATGCGCAATGGACGTAAGTCTGACGCTGCGACGTCGCGTGG2300.16105765864179378No Hit
AGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGCAGG2290.16035740795204684No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG2100.14705264484685518No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA2100.14705264484685518No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG2030.14215089001862666No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC1970.13794938588014508No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1840.128846126913435No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA1810.12674537484419424No Hit
TGAGGAATATTGCTCAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGC1730.12114336932621879No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1630.11414086242874949No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1590.11133985966976177No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1570.10993935829026792No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG1480.10363710208254553No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1460.10223660070305168No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC1440.10083609932355782No Hit
TGAGGACTATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1430.1001358486338109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCCAC100.0018291287228.09
TAACGGA100.0018291287228.05
TATTGCT400.0228.08
TATTGCC152.0205864E-5228.08
GCTATTG152.0205864E-5228.06
ATTGCTT202.2284621E-7228.09
ATTGCTC202.2284621E-7228.09
ATTGCCC152.0205864E-5228.09
ATTGCAT100.0018291287228.09
TCTTGCG1250.0228.08
TATTCCT202.2284621E-7228.08
ATCATGG202.2284621E-7228.07
AATATAG100.0018291287228.06
ATTCCTC202.2284621E-7228.09
AGTCTAC152.0205864E-5228.09
TGAATAT100.0018291287228.04
GCTTCCA100.0018291287228.08
TCGAGAG202.2284621E-7228.01
TTTGCAC202.2284621E-7228.09
CTTCCAC152.0205864E-5228.09
Produced by FastQC (version 0.11.9)