FastQCFastQC Report
Wed 30 Nov 2022
4061-JW07trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW07trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108732
Sequences flagged as poor quality0
Sequence length234
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA3216829.584666887392856No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG1429613.147923334436964No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA36133.322848839348122No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG30422.7977044476327118No Hit
TAAGGAATATTGCGCAATGGGCGACAGCCTGACGCAGCGACGCCGCGTGG19051.7520141264761064No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA18481.6995916565500497No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA11361.0447706287017622No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA7940.7302358091454217No Hit
TAAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG7670.7054041128646581No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA7560.6952874958613839No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA6510.5987197881028584No Hit
TCGAGAATCTTCGGCAATGGGCGCAAGCCTGACCGAGCGACGCCGCGTGC5920.5444579332671156No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC5900.5426185483574293No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA5820.5352610087186844No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG5560.5113490048927639No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAAGACCGCGTGG5420.49847331052496047No Hit
TGGGGAATCTTGGTCAATGGGCGCAAGCCTGAACCAGCGACGCCGCGTGA5230.48099915388294157No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGG5120.47088253687966747No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC4710.43317514623110026No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGA4150.3816723687598867No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG4140.3807526763050436No Hit
TGAGGAATATTGCTCAATGGGCGCAAGCCTGAAGCAGCGACGCCGCGTGG4130.3798329838502005No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG4060.3733951366662988No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA3830.3522422102049075No Hit
TAGGGAATATTGGTCAATGGATGGAAGTCTGAACCAGCCATGCCGCGTGC3820.3513225177500644No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGA3770.34672405547584884No Hit
TCGAGAATCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGC3700.34028620829194717No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGC3600.33108928374351615No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGA3340.3071772799175955No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA3260.2998197402788508No Hit
TGAGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGG3260.2998197402788508No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG3240.29798035536916456No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA3180.2924622006401059No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGG3180.2924622006401059No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC3160.2906228157304197No Hit
TAACGAATCTTCCACAATGCACGAAAGTGTGATGGAGCGACGCCGCGTGT3010.27682742890777323No Hit
TGGGGAATTTTCTGCAATGGGCGAAAGCCTGACAGAGCAATACCGCGTGA2890.26579111944965605No Hit
TCGAGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACGCCGCGTGG2620.24095942316889235No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCACGCCGCGTGA2510.23084280616561823No Hit
TCGAGGATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACACCGCGTGC2420.22256557407203031No Hit
CAAGGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGG2340.2152080344332855No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA2260.2078504947945407No Hit
TAAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGG2250.2069308023396976No Hit
TGAGGAATATTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGG2220.2041717249751683No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGT2210.20325203252032523No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA2100.1931354155170511No Hit
TAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA2090.192215723062208No Hit
TCGAGAATTTTTCTCAATGGGCGAAAGCCTGAAGGAGCGACGCCGCGTGG2000.18393849096862008No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGG1980.1820991060589339No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAACGCCGCGTGG1950.1793400286944046No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG1890.17382187396534599No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAACGCCGCGTGG1890.17382187396534599No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGT1890.17382187396534599No Hit
TGAGGGATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGG1870.1719824890556598No Hit
TAACGAATATTCCGCAATGCACGAAAGTGTGACGGAGCAATGCCGCGTGT1860.1710627966008167No Hit
TCGAGGATCTTCGGCAATGGGCGCAAGCCTGACCGAGCGACGCCGCGTGT1820.1673840267814443No Hit
TGGGGAATCTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGG1760.16186587205238567No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1730.15910679468785638No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG1730.15910679468785638No Hit
TGGGGAATTTTTCGCAATGGGGGAAACCCTGACGAAGCAACGCCGCGTGG1720.1581871022330133No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1640.1508295625942685No Hit
TGAGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGG1630.1499098701394254No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCGACGCCGCGTGT1620.14899017768458228No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1590.14623110032005296No Hit
TGAGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA1570.14439171541036677No Hit
TAAGGAATATTGGTCAATGGACGAAAGTCTGAACCAGCCATGCCGCGTGG1570.14439171541036677No Hit
TGGGGAATATTGGACAATGGGGGCATCCCTGATCCAGCAATGCCGCGTGA1560.1434720229555237No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGG1480.1361144833167789No Hit
TTCGGAATATTCGGCAATGGACGAAAGTCTGACCGAGCGACGCCGCGTGC1450.13335540595224957No Hit
TGGGGAATTTTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA1450.13335540595224957No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGGGGG1430.13151602104256335No Hit
TGGGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGT1420.13059632858772027No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGT1420.13059632858772027No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA1410.12967663613287717No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCACGCCGCGTGA1400.12875694367803409No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC1400.12875694367803409No Hit
TCGAGAGGCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGG1390.12783725122319098No Hit
TGGGGAATATTGGGCAATGGAGGCAACTCTGACCCAGCCATGCCGCGTGG1390.12783725122319098No Hit
TGGGGAATCTTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGG1380.12691755876834787No Hit
TAGGGAATATTGGTCAATGGATGCAAGTCTGAACCAGCCATGCCGCGTGC1360.12507817385866166No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGT1360.12507817385866166No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGT1340.12323878894897546No Hit
TCGAGAATCATTCGCAATGGGCGAAAGCCTGACGGTGCGACGCCGCGTGG1280.11772063421991687No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGA1250.11496155685538756No Hit
TGGGGAGTATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGG1240.11404186440054445No Hit
TAGGGAATATTGGACAATGGGTGCGAGCCTGATCCAGCCATGCCGCGTGC1240.11404186440054445No Hit
TAGGGAATATTGGACAATGGGTGAGAGCCTGATCCAGCCATGCCGCGTGC1230.11312217194570137No Hit
TCGAGAATAATTCACAATGGGGGCATCCCTGATGGTGCAACGCCGCGTGG1220.11220247949085826No Hit
TCGAGAATCTTCCGCAATGGGCGCAAGCCTGACGGAGCGACGCCGCGTGT1180.10852370967148585No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT1170.10760401721664276No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGT1150.10576463230695654No Hit
TCGGGAATTTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGA1130.10392524739727035No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCATGCCGCGTGA1130.10392524739727035No Hit
TAAGGAATATTGGACAATGGGCGGAAGCCTGATCCAGCCATGCCGCGTGA1120.10300555494242726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCT100.001828695227.99318
TCTTGCA850.0227.99318
AATTTTA152.0195072E-5227.99316
TTTACGC152.0195072E-5227.99319
CTTCCAC202.2263703E-7227.99319
TCATTCA152.0195072E-5227.99318
TCTTCCA202.2263703E-7227.99318
ATTCGAC152.0195072E-5227.99319
CATTCAC152.0195072E-5227.99319
TCTAGAG202.2263703E-7227.99311
CTTGGTC900.0227.99319
TGTTGCG202.2263703E-7227.99318
ATTCCAC152.0195072E-5227.99319
GGGACTT400.0227.99313
ATTTTTG100.001828695227.99317
ATTTTGC1400.0227.99317
ATTTTAC152.0195072E-5227.99317
GAGAATC3550.0227.99313
CTTGCAC850.0227.99319
TATTCGA152.0195072E-5227.99318
Produced by FastQC (version 0.11.9)