FastQCFastQC Report
Wed 30 Nov 2022
4061-JW07trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW07trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108732
Sequences flagged as poor quality0
Sequence length232
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2654324.41139682890042No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA1359012.498620461317737No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT28042.5788176433800536No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA25932.3847625354081594No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG19491.7924805944892028No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA16401.5082956259426847No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTGCGGCCTAGCA15881.4604716182908435No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA10180.9362469190302763No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA9800.9012986057462384No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA8590.7900158187102232No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA8200.7541478129713424No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG5290.48651730861200015No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTCTGGTCCAGTA4900.4506493028731192No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTTAGCGTCAGGTACGGCCCAGCG4620.42489791413751243No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG4330.3982268329470625No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTATCGTCCAGAA4040.3715557517566126No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG3620.33292866865320236No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA3600.33108928374351615No Hit
CCCGTTTGCTACCTTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTA3510.32281205164992827No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCAGGTCAAGCCCAGGA3310.30441820255306623No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA3190.29338189309494905No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG3150.2897031232755766No Hit
CCCAGTTGCTCCCCTAGCTTTCGTCTCTTAGTGTCAGTTTTGGCCCAGCA3100.28510466100136117No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA3020.2777471213626163No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2940.27038958172387156No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA2940.27038958172387156No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTTAGTGTCAGTAATGGCCCAGTA2930.26946988926902843No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA2840.26119265717544055No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG2810.25843357981091125No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG2700.24831696280763713No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTTCGGCCCAGAG2660.2446381929882647No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCTTAGTA2630.24187911562373543No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2590.238200345804363No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCGCTGTCCAGAA2520.2317624986204613No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2450.2253246514365596No Hit
CCCGTTTGCTCCCCTAGCTTTCGTTCCTCAGCGTCAGAAGAGACCCAGTG2420.22256557407203031No Hit
CCCGTTTGCTACCCTAGCTTTCGCACCTCAGCGTCAGATTTGGCCCAGTG2390.219806496707501No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG2260.2078504947945407No Hit
CCTGTTTGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTGTTCGTCCAGTT2220.2041717249751683No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA2030.1866975683331494No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTGTGGACCAGAG1990.183018798513777No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA1960.18025972114924768No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1940.1784203362395615No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1940.1784203362395615No Hit
CCCTTTCGCTCCCCCGGCCTTCGAGCCTCAGCGTCAGCAATTGTCCAGCA1890.17382187396534599No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1890.17382187396534599No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGCGTTAGTAATAGCCCAGTA1850.17014310414597358No Hit
CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG1830.16830371923628737No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATTGTCCAGAG1820.1673840267814443No Hit
CCCGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGGCCAAGGCCAGGG1790.164624949416915No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCT1690.15542802486848398No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGTGTCAATATATGTGTAGCC1640.1508295625942685No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGGG1630.1499098701394254No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTACAGCGTCAGAAACGAGCCAGTG1620.14899017768458228No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG1590.14623110032005296No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA1580.14531140786520985No Hit
CCCGTTCGCTACCCATGCTTTCGTCCCTGAGCGTCAACAACGGCCCAGCC1550.14255233050068059No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTCAGTAATAGCCCAGTA1520.13979325313615126No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1490.13703417577162197No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGGAACTGCCCAGAA1480.1361144833167789No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTATCGGCCCAGCA1450.13335540595224957No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGACCCAGAT1440.13243571349740646No Hit
CCCGTTCGCTACCCATGCTGTCGCGTCTTAGCGTCAGGTACAGCCCAGCG1430.13151602104256335No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTG1390.12783725122319098No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTGTTGTGCCAGGG1380.12691755876834787No Hit
CCTGTTTGCTACCCGCGCTTTCGCGTCTCAGCGTCAGTACCTGTCCAGGT1340.12323878894897546No Hit
CCTGTTTGCTCCCCACACTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT1270.11680094176507376No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA1220.11220247949085826No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1200.11036309458117206No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAAAAATTGCCCAGTT1180.10852370967148585No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG1160.10668432476179965No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTGCACTCTGGTA1140.10484493985211346No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA1120.10300555494242726No Hit
CCCGTTTGCTCCCCATGCTGTCGCGCTTTAGCGTCAGGTATGGCCCAGCG1100.10116617003274105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCT850.0225.99684
TTGCTAC39350.0225.996786
GGTTTGC600.0225.996783
TTTTTGC350.0225.996783
TGTTCGC1950.0225.996783
TGTTCGA152.0916204E-5225.996783
CTTTGCT252.6011548E-9225.996784
CTATTTG302.910383E-11225.996782
TTTGCTA39350.0225.996785
CCTATTT302.910383E-11225.996781
CATTTGC34550.0225.996783
ATTTGCT35000.0225.996784
GTTGCTC302.910383E-11225.996785
GTTTGTT100.0018774766225.996774
GTTTGAT2100.0225.996774
ATTCGCT2050.0225.996774
CCTGTTC2100.0225.996771
CGATTCG100.0018774766225.996772
CTTGCTC202.32616E-7225.996775
TCGCTGC202.32616E-7225.996776
Produced by FastQC (version 0.11.9)