FastQCFastQC Report
Wed 30 Nov 2022
4061-JW08trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW08trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139727
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG7966257.012603147566324No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG30952.2150336012366973No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC20801.4886170890379096No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG19971.4292155417349546No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC17461.249579537240476No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA13260.9489933942616675No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA13200.9446993065048273No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC10770.7707887523528022No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG9380.6713090526526727No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG7470.5346139257265954No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG6800.4866632791085474No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA5920.42368332534155884No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG5250.37573267872351085No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4880.3492524708896634No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG4560.3263506695198494No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA4500.3220565817630093No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA4480.32062521917739595No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC4230.3027331868572287No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG4070.2912822861723217No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA3950.2826941106586415No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG3900.27911570419460807No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA3630.2597923092888275No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA3620.2590766279960208No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG3600.25764526541040744No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG3390.24261595826146698No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG3370.24118459567585362No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA3220.2304493762837533No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG3130.22400824464849314No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG3130.22400824464849314No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC3040.21756711301323295No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG3030.21685143172042626No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG3030.21685143172042626No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA2820.20182212457148582No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG2750.19681235552183898No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA2670.1910869051793855No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA2650.18965554259377213No Hit
TGAGGAATATTGCTCAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGC2620.18750849871535208No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG2500.17892032320167184No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA2470.17677327932325176No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA2420.17319487285921834No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT2420.17319487285921834No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2380.17033214768799157No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2330.16675374122395814No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2270.16245965346711802No Hit
TAGGGAATATTAGTTAATGTGCGAAAGCGCGAACTAGCAACGCCGCGTGT2220.1588812470030846No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG2170.15530284053905113No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1990.1424205772685308No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT1840.13168535787643046No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG1830.1309696765836238No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG1820.1302539952908171No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1770.12667558882678365No Hit
TGAGGAATATTGGTCAATGGGCGTAAGCCTGAACCAGCCATCCCGCGTGA1740.12452854494836359No Hit
TCGAGAATAATTCGCAATGGACGAAAGTCTGACGATGCGACGCCGCATGA1710.12238150106994353No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC1690.12095013848433016No Hit
TCGAGAGGCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGG1640.11737173202029672No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1570.11236196297064992No Hit
TCGAGAATATTCGTCAATGCGCGAAAGCGTGAACGAGCGACGCCGCGTGC1570.11236196297064992No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC1560.11164628167784321No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGA1530.10949923779942315No Hit
TGAGGACTATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1480.10592083133538971No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1460.10448946874977635No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG1410.10091106228574291No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1410.10091106228574291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATTA350.0228.000026
GAATTGT350.0228.000025
TAATATT202.2282984E-7228.05
ACTATTG600.0228.06
TAGAGGC202.2282984E-7228.03
ATTAGTT252.4701876E-9228.09
GTCGATA100.0018290863228.09
AGGAGTA202.2282984E-7228.03
CGAAACC252.4701876E-9228.04
CATTGCG152.0204927E-5228.08
ACATTGC202.2282984E-7228.07
ATTGTTC302.7284841E-11228.07
GAATATA100.0018290863228.05
TTTCCAC100.0018290863228.09
CATTCGC100.0018290863228.09
AATATTT100.0018290863228.06
AATATAG100.0018290863228.06
ATTCCTC100.0018290863228.09
TATTGAG152.0204927E-5228.08
AACATTG252.4701876E-9228.06
Produced by FastQC (version 0.11.9)