FastQCFastQC Report
Wed 30 Nov 2022
4061-JW08trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW08trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139727
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA6460046.233011515312No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA78925.648156762830377No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA48663.4825051707973405No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA37582.689530298367531No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT22811.632469028892054No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC20321.4542643869831886No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA14701.0520515004258304No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG9980.7142499302210739No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA9250.6620051958461858No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT8680.6212113621562045No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA7140.5109964430639747No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA6840.4895260042797741No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT5810.41581083112068534No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA4640.3320761198623029No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC4170.2984390991003886No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG4070.2912822861723217No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA3690.2640863970456676No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3660.2619393531672476No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3520.25191981506795397No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG3290.23545914533340015No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3270.23402778274778674No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG3210.22973369499094662No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT2980.21327302525639283No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT2960.21184166267077942No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2810.20110644327867916No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGACCCAGGA2780.19895939940025908No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA2670.1910869051793855No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG2420.17319487285921834No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG2400.17176351027360495No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT2340.16746942251676483No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA2090.14957739019659766No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA2050.1467146650253709No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1990.1424205772685308No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG1900.13597944563327058No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1900.13597944563327058No Hit
CCTGTTTGCTCCCCTTGCTTTCGTGCCTCAGCGTCAGGAGTAGGCTAGAA1830.1309696765836238No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1780.12739127011959034No Hit
CCCGTTCGCTACCCTAGCTTTCGAGTCTGAGTGTCAGGATCAGACCAGGG1750.1252442262411703No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA1710.12238150106994353No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1670.11951877589871679No Hit
CCCATTCGCTACCCACGCTTTCGCTGCTCAGTGTCAGGAGTGTGCCAATC1590.1137933255562633No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT1550.11093060038503653No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT1550.11093060038503653No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA1550.11093060038503653No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA1490.10663651262819641No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTTAGCGTCAGGTCAGGCCCAGCG1460.10448946874977635No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA1450.10377378745696966No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA1440.10305810616416297No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG1430.1023424248713563No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTCTTGGTCCAGAA1420.1016267435785496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC103900.0226.000023
CTGTGTG550.0226.000022
GTTTGTT100.001877968226.04
TGTTTGT100.001877968226.03
TTGATAC202.3283428E-7226.06
GTTTGAT1500.0226.04
TTGGCTA100.001877968226.05
TTTACTA152.0927753E-5226.05
CTTTTTG350.0226.02
TTTGTTC100.001877968226.05
CTTTTGC100.001877968226.03
TTGCTGC302.910383E-11226.06
GTTTACT152.0927753E-5226.04
CGGTTCG3950.0226.02
TATGCTC100.001877968226.05
GCTTGAT100.001877968226.04
TGGTTGC302.910383E-11226.03
GTTCGAT1750.0226.04
CCTTTTG100.001877968226.02
TGATTGC100.001877968226.03
Produced by FastQC (version 0.11.9)