FastQCFastQC Report
Wed 30 Nov 2022
4061-JW09trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW09trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46839
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1916140.908217511048484No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC12382.643096564828455No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC12152.593992185998847No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG6761.443241742991951No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG5661.2083947138068702No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG5521.1785050919105873No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA5501.1742351459254041No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4070.8689340079847989No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA4040.8625290890070241No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC3960.8454493050662908No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA3800.8112897371848246No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG3170.6767864386515511No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA2870.6127372488738018No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA2300.49104378829607803No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG2190.46755908537756996No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2080.44407438245906194No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG2000.4269945985183287No Hit
GGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1850.39497000362945406No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1820.38856508465167916No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG1780.3800251926813126No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG1570.33519075983688806No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA1430.30530113794060504No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1390.29676124597023845No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC1360.29035632699246355No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA1350.2882213539998719No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1310.2796814620295053No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1310.2796814620295053No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC1190.25406178611840563No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1150.24552189414803902No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG1080.23057708319989753No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG1070.22844211020730587No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA1050.22417216422212258No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG920.19641751531843124No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA900.19214756933324792No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG870.185742650355473No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG870.185742650355473No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA780.16652789342214822No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG750.1601229744443733No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT750.1601229744443733No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA740.15798800145178163No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC720.15371805546659834No Hit
AGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG720.15371805546659834No Hit
TGAGGACTATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG710.1515830824740067No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA710.1515830824740067No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGGGG700.14944810948141507No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT670.14304319050364014No Hit
TGAGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG640.1366382715258652No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG640.1366382715258652No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA640.1366382715258652No Hit
TGAGGAATAGTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG640.1366382715258652No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG590.12596340656290697No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC580.12382843357031532No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG570.1216934605777237No Hit
TGAGGAATATTGCGCAATGGTCGAAAGCCTGACGCAGCAACGCCGCGTGG570.1216934605777237No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA560.11955848758513204No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG540.11528854159994877No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA530.11315356860735712No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC510.10888362262217383No Hit
CTAAGAATCTTCCGCAATGGGGGAAACCCTGACGGAGCGACGCCGCGTGG500.10674864962958218No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC500.10674864962958218No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACTCAGCAACGCCGCGTGG490.10461367663699055No Hit
TAGGGAATATTGGTTAATCTGCGAAAGCGGGAACCAGCAACGCCGCGTGC490.10461367663699055No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA480.1024787036443989No Hit
CAAGGAATTTTCCCCAATGGACGCAAGTCTGAGGGAGCAACGCCGCGTGG470.10034373065180724No Hit
TGAGGAATATTGAGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG470.10034373065180724No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT470.10034373065180724No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC470.10034373065180724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATA1450.0228.000023
ATTTTGC3600.0228.000027
AATCTTG1050.0228.06
ATCTTGC1050.0228.07
AATTTTT202.2126005E-7228.06
AGGACTA202.2126005E-7228.03
GGACTTT100.0018252127228.04
TTTCCGC252.4447218E-9228.09
TTTCCAC152.0119443E-5228.09
GAAGCTT100.0018252127228.05
TTTTCGC152.0119443E-5228.09
TGTTCGC202.2126005E-7228.09
ATTGGAC600.0228.09
TATTGGA650.0228.08
GATTGCA100.0018252127228.08
GAGTAAT100.0018252127228.02
TCTTGCG1100.0228.08
ATGTTGC152.0119443E-5228.07
TAATATT100.0018252127228.05
TTTGGAC100.0018252127228.09
Produced by FastQC (version 0.11.9)