FastQCFastQC Report
Wed 30 Nov 2022
4061-JW10trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW10trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83638
Sequences flagged as poor quality0
Sequence length234
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1983023.709318730720486No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG58947.047036036251465No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC36834.403500801071283No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG30293.621559578182166No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG18862.254955881297975No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA18722.2382170783615103No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG17472.088763480714508No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC16091.9237667089122168No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA10921.3056266290442142No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA8671.0366101532796097No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG7740.9254166766302397No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA7730.9242210478490639No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG7320.875200267820847No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA7210.8620483512279108No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG6830.8166144575432219No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG6150.7353117004232526No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG5950.7113991247997321No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC5220.6241182237738827No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4650.5559673832468496No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA4550.5440110954350893No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG4420.528467921279801No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4090.48901217150099235No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG3830.45792582319041586No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG3820.45673019440923984No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCATGCCGCGTGC2870.3431454601975179No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC2870.3431454601975179No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG2830.3383629450728138No Hit
TGGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGA2660.31803725579282144No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA2540.3036897104187092No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG2540.3036897104187092No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG2520.30129845285635715No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT2520.30129845285635715No Hit
TGGGGAATCTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG2420.2893421650445969No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT2320.27738587723283675No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG2260.27021210454578065No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA2190.2618427030775485No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC2130.2546689303904924No Hit
TGAGGAATATTGCGCAATGGACGAATGTCTGACGCAGCAACGCCGCGTGG2000.23912575623520407No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG1810.2164088093928597No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1800.21521318061168368No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC1780.21282192304933165No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1750.20923503670580357No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA1620.19369186255051532No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG1590.19010497620698727No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1590.19010497620698727No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1580.18890934742581122No Hit
TGGGGAATATTGCGCAATAGGGGAAACCCTGACGCAGCGACGCCGCGTGG1570.1877137186446352No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1530.18293120351993114No Hit
TGAGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCGACGCCGCGTGA1480.17695305961405103No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC1460.17456180205169897No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1440.17217054448934696No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA1420.1697792869269949No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC1420.1697792869269949No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG1420.1697792869269949No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1380.16499677180229083No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1350.16140988545876278No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG1350.16140988545876278No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA1340.16021425667758676No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1320.1578229991152347No Hit
TGAGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1300.15543174155288267No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG1250.14945359764700256No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG1210.14467108252229846No Hit
TGAGGAATATTGCACAATAGGCGCAAGCCTGATGCAGCAACGCCGCGTGG1200.14347545374112244No Hit
CTAAGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGA1200.14347545374112244No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA1200.14347545374112244No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG1130.13510605227289033No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC1130.13510605227289033No Hit
CTAAGAATCTTCCGCAATGGGGGAAACCCTGACGGAGCGACGCCGCGTGG1110.13271479471053826No Hit
TGGGGAATTTTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG1070.1279322795858342No Hit
CAAGGAATTTTCCCCAATGGGCGCATGCCTGAGGGAGCAACGCCGCGTGG1060.12673665080465815No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGA1040.12434539324230613No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG1040.12434539324230613No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGT990.11836724933642602No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGT960.11478036299289796No Hit
TCGAGAATCTTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGA930.1111934766493699No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA930.1111934766493699No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG900.10760659030584184No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA880.1052153327434898No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG860.10282407518113776No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC860.10282407518113776No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT860.10282407518113776No Hit
TAAGGGATATTGCGACAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG860.10282407518113776No Hit
GGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGGATG860.10282407518113776No Hit
TAGGGAATATTGCACAATGAGCGCAAGCTTGATGCAGCAACGCCGCGTGC840.10043281761878571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCC152.017748E-5227.995829
CTTCCAC152.017748E-5227.995829
TCTTCCC152.017748E-5227.995828
TCTTCCA152.017748E-5227.995828
AACTTTA152.017748E-5227.995828
GGATCTT152.017748E-5227.995825
AATATTA152.017748E-5227.995826
TGTTCGC152.017748E-5227.995829
GATATTG3850.0227.995826
TATTAGT152.017748E-5227.995828
ATTGGTT600.0227.995829
TTTGCAC152.017748E-5227.995829
AGGATCT152.017748E-5227.995824
ATTGCGA1700.0227.995829
ATCATTC152.017748E-5227.995827
AGAATCT650.0227.995824
ATATTGA152.017748E-5227.995827
ATATTAG152.017748E-5227.995827
GTTTGCG100.0018278767227.99588
TCTTGCC100.0018278767227.99588
Produced by FastQC (version 0.11.9)