FastQCFastQC Report
Wed 30 Nov 2022
4061-JW10trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW10trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83638
Sequences flagged as poor quality0
Sequence length232
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA2079424.86190487577417No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG19992.390061933570865No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT19822.3697362442908725No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA12501.4945359764700257No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG12211.459862741815921No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA12161.4538845979100408No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT12121.4491020827853367No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT12021.4371457949735766No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA6010.7185728974867883No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA5860.700638465769148No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA5630.6731390038020996No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC5070.6061837920562424No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA4660.5571630120280255No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA4010.4794471412515843No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3900.46629522465864803No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC3780.4519476792845358No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA3460.41368755828690307No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA3420.408905043162199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3380.4041225280374949No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA2690.32162414213634954No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2660.31803725579282144No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA2600.31086348310576534No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG2600.31086348310576534No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA2590.3096678543245893No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2440.291733422606949No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA2160.2582558167340204No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA2110.2522776728281403No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2010.24032138501638012No Hit
CCTGTGTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1980.23673449867285204No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1970.23553886989167602No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA1970.23553886989167602No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG1930.23075635476697193No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1890.2259738396422679No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1830.21880006695521176No Hit
CCCGTTCGCTCCCCGTGCTTTCGCATCTGAGCGTCAGTATAGGTCCAGCA1780.21282192304933165No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG1770.21162629426815563No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAACAGCTTAGCA1770.21162629426815563No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG1760.2104306654869796No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1730.20684377914345153No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT1720.20564815036227552No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1660.1984743776752194No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1660.1984743776752194No Hit
CACTGACGGATTTTACCGTCCACGACTCTCTGAACTCCTCAAACCAGGAA1630.19488749133169134No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA1630.19488749133169134No Hit
CCTGTTTGCTCCCCACGCGTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1610.19249623376933928No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1600.1913006049881633No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCCTGAGCGTCAGTTCTCGACCAGAA1600.1913006049881633No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG1550.18532246108228317No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT1550.18532246108228317No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCAGTCCAGGA1400.16738802936464287No Hit
CCTGGTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1370.1638011430211148No Hit
CCCGTTCGCTACCCATGCTTTCGCGTCTTAGCGTCAGGTACAGCCCAGGG1340.16021425667758676No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTCGTCCAGAA1340.16021425667758676No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA1320.1578229991152347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTGTCGGTCCAGGA1310.15662737033405869No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1310.15662737033405869No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA1300.15543174155288267No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1270.1518448552093546No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTCAGCGTCAGGTACGGTCCAGGA1240.14825796886582654No Hit
CCTGTTTTCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1230.14706234008465052No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGCGTCAGGATCAGTCCAGAA1140.13630168105406634No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA1130.13510605227289033No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1110.13271479471053826No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1110.13271479471053826No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGATGCAGTCCAGAA1110.13271479471053826No Hit
CCGGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1110.13271479471053826No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG1070.1279322795858342No Hit
CCTGTTCGCTCCCCACGCTTTCGCATATCAGCGTCAATGTTAGGCCAGAG1070.1279322795858342No Hit
CCTGTTTGCTCCCCAAGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1050.12554102202348214No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA1040.12434539324230613No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT1000.11956287811760204No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA990.11836724933642602No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGCCCAGAA960.11478036299289796No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA950.11358473421172195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT920.10999784786819389No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCTAGGA910.10880221908701787No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG900.10760659030584184No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGGT900.10760659030584184No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG890.10641096152466582No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA890.10641096152466582No Hit
CCTGCTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA880.1052153327434898No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACGGGCCCAGCG870.10401970396231378No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA870.10401970396231378No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA860.10282407518113776No Hit
CCCGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGCTGGGCCAGTG850.10162844639996174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG252.586603E-9226.123292
TTGCTGC450.0226.123296
TGGTTGC450.0226.123293
CTGTTCG4250.0226.123292
GGTTGCT500.0226.123294
TTTGATG252.586603E-9226.123295
CGATCGC252.586603E-9226.123293
CCGTTCG8550.0226.123292
TTGATGC152.0852338E-5226.123286
TGTTTGC47650.0226.123283
GTTTGCG202.316483E-7226.123284
TTGGCTC100.0018735601226.123285
ACTGACG100.0018735601226.123282
TTGCTTC100.0018735601226.123286
CGGTTCG4350.0226.123282
GGTTTGC600.0226.123283
TGACGGA100.0018735601226.123284
CTGACGG100.0018735601226.123283
GTTCGAT2150.0226.123284
GTATGCT152.0852338E-5226.123284
Produced by FastQC (version 0.11.9)