FastQCFastQC Report
Wed 30 Nov 2022
4061-JW11trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW11trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143459
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG5026535.03788538885675No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG86396.02192961055075No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC63574.431231222858099No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC25201.7565994465317616No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG22911.59697195714455No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG21361.4889271499173982No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG18121.2630786496490287No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA14631.0198035675698283No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA14080.9814650875860002No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA14070.9807680243135669No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA13770.9598561261405698No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA12560.8755114701761478No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG11090.7730431691284617No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA9540.6649983619013098No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG8880.618992185920716No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC7750.54022403613576No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG7340.5116444419659973No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG7060.49212667033786656No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC6980.4865501641584007No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG6310.43984692490537364No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC6000.4182379634599433No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA5840.4070849511010114No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG5810.4049937612837117No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG5720.39872019183181256No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA5070.35341107912365205No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG4940.34434925658202No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4720.3290138645884887No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC4590.31995204204685657No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT4320.30113133369115913No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA4170.2906753846046606No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA4020.280219435518162No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT3930.27394586606626287No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA3930.27394586606626287No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC3890.27115761297652985No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG3660.2551251577105654No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG3460.24118389226190062No Hit
TGAGGAATATTGCGCAATGGACGAATGTCTGACGCAGCAACGCCGCGTGG3440.23978976571703414No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG3420.23839563917216763No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG3420.23839563917216763No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGG3200.22306024717863643No Hit
TGGGGAATCTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG3000.20911898172997165No Hit
TGGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGA2980.20772485518510517No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA2930.20423953882293894No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG2880.20075422246077276No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC2850.19866303264347304No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG2750.19169239991914067No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCATGCCGCGTGC2680.18681295701210798No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC2610.18193351410507533No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2550.17775113447047589No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG2550.17775113447047589No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2480.1728716915634432No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA2470.17217462829101No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA2450.1707805017461435No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAATGCCGCGTGC2340.16311280574937786No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC2330.16241574247694462No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2290.15962748938721166No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2260.15753629956991197No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG2210.15405098320774577No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT2030.14150384430394747No Hit
TGGGGAATTTTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG2010.140109717759081No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2000.13941265448664775No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1930.13453321157961506No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG1920.13383614830718182No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC1870.13035083194501565No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGA1810.1261684523104162No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1760.12268313594825002No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA1720.11989488285851707No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1720.11989488285851707No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG1670.11640956649635087No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA1660.11571250322391763No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT1600.11153012358931821No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC1600.11153012358931821No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1590.11083306031688496No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC1550.10804480722715201No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGT1540.10734774395471877No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1530.10665068068228553No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCCGACGCAGCAACACCGCGTGG1520.10595361740985229No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATACCGCGTGT1500.10455949086498582No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC1490.10386242759255258No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGT1460.10177123777525285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGGAC2300.0228.000029
ATATTCC1850.0228.000027
GAGGACT252.4701876E-9228.02
TCTTGCC100.0018291371228.08
ATTAGTT252.4701876E-9228.09
GTATTGC100.0018291371228.07
CTTCCAC100.0018291371228.09
TCTTCCA100.0018291371228.08
ATTACGC100.0018291371228.09
AGGACTA252.4701876E-9228.03
ACTTCAC100.0018291371228.09
TTCGTCG202.2284985E-7228.06
ATTCGAC100.0018291371228.09
GAATATA100.0018291371228.05
TTTCCAC202.2284985E-7228.09
CATTCGC100.0018291371228.09
ATCGTAC100.0018291371228.07
CTTGGAC302.7284841E-11228.09
TATTGCT400.0228.08
TATTGCC100.0018291371228.08
Produced by FastQC (version 0.11.9)