FastQCFastQC Report
Wed 30 Nov 2022
4061-JW13trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW13trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99398
Sequences flagged as poor quality0
Sequence length234
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA3004430.225960280890963No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG56065.639952514135093No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA38333.8562144107527314No Hit
TAAGGAATATTGCGCAATGGGCGACAGCCTGACGCAGCGACGCCGCGTGG29722.9899997987887077No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG17451.7555685225054831No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA17391.7495321837461517No Hit
TAACGAATCTTCCACAATGCACGAAAGTGTGATGGAGCGACGCCGCGTGT15971.6066721664419807No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA11461.1529407030322543No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGA9770.9829171613110927No Hit
TGGGGAATCTTGGTCAATGGGCGCAAGCCTGAACCAGCGACGCCGCGTGA7360.7404575544779574No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG7320.7364333286384032No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA6840.6881426185637538No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA6380.6418640214088815No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC6210.6247610615907765No Hit
TCGAGAATTTTTCTCAATGGGCGAAAGCCTGAAGGAGCGACGCCGCGTGG6010.604639932393006No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA6010.604639932393006No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC5550.5583613352381336No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA5470.5503128835590254No Hit
TGAGGAATATTGCTCAATGGGCGCAAGCCTGAAGCAGCGACGCCGCGTGG5300.5332099237409204No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC5250.5281796414414777No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGG5010.504034286404153No Hit
TGAGGAATATTCGGCAATGGGGGCAACCCTGACCGAGCAACGCCGCGTGG4340.43662850359162153No Hit
TAAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG4120.41449526147407395No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG3990.40141652749552303No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGG3820.3843135676774181No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCATGCCGCGTGA3750.37727117245819836No Hit
TGAGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA3430.34507736574176545No Hit
TAGGGAATATTGGTCAATGGATGGAAGTCTGAACCAGCCATGCCGCGTGC3370.33904102698243427No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA3330.3350168011428801No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGG3300.33199863176321454No Hit
TAGGGAATATTGGACAATGGGTGCGAGCCTGATCCAGCCATGCCGCGTGC3290.33099257530332604No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA3030.30483510734622427No Hit
TAAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGG2970.2987987685868931No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG2970.2987987685868931No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA2960.2977927121270046No Hit
TGGGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGT2900.2917563733676734No Hit
TGGGGAATCTTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGG2650.2666049618704602No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATCTCGCGTGA2550.25654439727157485No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGA2410.24245960683313547No Hit
TGGGGAATTTTGGGCAATGGCCGAAAGGCTGACCCAGCAACGCCGCGTGA2350.23642326807380432No Hit
TCGAGAATCTTCGGCAATGGGCGCAAGCCTGACCGAGCGACGCCGCGTGC2240.22535664701503047No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA2230.22435059055514198No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC2210.2223384776353649No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCCACGCCGCGTGA2150.2163021388760337No Hit
TAACGAATATTCCGCAATGGACGAAAGTCTGACGGAGCAATGCCGCGTGC2040.2052355178172599No Hit
TCGAGAATCATTCGCAATGGGCGAAAGCCTGACGGTGCGACGCCGCGTGG2030.20422946135737136No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG1890.19014467091893197No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAAGACCGCGTGG1840.18511438861948934No Hit
TCGAGGATCTTCGGCAATGGGGGCAACCCCGACCGAGCGATGCCGCGTGG1790.18008410632004668No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT1770.1780719934002696No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1740.17505382402060404No Hit
TGGGGAATTTTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA1730.17404776756071552No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGC1730.17404776756071552No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG1710.17203565464093845No Hit
TCGAGAATCTTTCACAATGGGGGCAACCCTGATGGAGCGACGCCGCGTGG1620.1629811465019417No Hit
TTAAGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA1620.1629811465019417No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGG1600.16096903358216463No Hit
TAACGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGT1540.15493269482283345No Hit
TGGGGAATATTGGACAATGGGGGCATCCCTGATCCAGCAATGCCGCGTGA1520.15292058190305638No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCACGCCGCGTGA1490.14990241252339082No Hit
TGGGGAATTTTCCGCAAGGGGCGAAAGCCTGACGGAGCAATACCGCGTGA1460.14688424314372522No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG1450.1458781866838367No Hit
TGGGGACTTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA1450.1458781866838367No Hit
TCGAGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACACCGCGTGC1360.13682367854483993No Hit
TCGAGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACGCCGCGTGG1360.13682367854483993No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA1340.13481156562506286No Hit
TAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA1300.13078733978550877No Hit
TCGAGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGC1290.12978128332562022No Hit
TCGAGAGGCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGG1280.1287752268657317No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGT1250.1257570574860661No Hit
TGGGGAATCTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGG1210.12173283164651201No Hit
TAAGGAATATTGGTCAATGGACGAAAGTCTGAACCAGCCATGCCGCGTGG1180.11871466226684642No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGA1160.11670254934706935No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA1150.11569649288718084No Hit
CAAGGAATTTTCCCCAATGGGCGAGAGCCTGAGGGAGCAACGCCGCGTGG1110.1116722670476267No Hit
GGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA1100.11066621058773818No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGC1070.1076480412080726No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCACGCCGCGTGA1050.10563592828829553No Hit
TCGAGGATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACACCGCGTGC1050.10563592828829553No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG1020.10261775890862995No Hit
TGGGGAATTTTTCGCAATGGGCGCAAGCCTGACGAAGCAACGCCGCGTGG1000.1006056459888529No Hit
TGGGGAGTATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGG1000.1006056459888529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAC100.0018283777227.996499
AATTTTA202.2252425E-7227.996496
AGTTTCC100.0018283777227.996497
AACTTTC100.0018283777227.996496
TAGAAGC100.0018283777227.996493
GATTTTT100.0018283777227.996496
ACTTCAC100.0018283777227.996499
TTTCCCC350.0227.996499
TATTGTA202.2252425E-7227.996498
TATTGCA1900.0227.996498
AAGTTTC100.0018283777227.996496
TATTCGT100.0018283777227.996498
GTTCCGC100.0018283777227.996499
GAATTGT700.0227.996495
ATTGCAC1900.0227.996499
ATGTTCC202.2252425E-7227.996497
TTTTCCT100.0018283777227.996498
TATTGCT750.0227.996488
GAGAATT1150.0227.996483
GATATTG450.0227.996486
Produced by FastQC (version 0.11.9)