FastQCFastQC Report
Wed 30 Nov 2022
4061-JW13trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW13trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99398
Sequences flagged as poor quality0
Sequence length232
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2323723.377733958429747No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA73507.394514980180687No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT39784.002092597436569No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG32403.2596229300388337No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA32173.2364836314613976No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTATGGCCCAGCA28742.8914062657196324No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA19131.924586007766756No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATGGCCCAGTA17931.8038592325801321No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA15011.5100907462926818No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA14691.477896939576249No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGATAAGGTCCAGCA12431.2505281796414416No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA7550.7595726272158394No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTATCGTCCAGAA6520.6559488118473209No Hit
CCCGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGGG6380.6418640214088815No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG6340.6378397955693274No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTGAGTGTCAGTTCTGGTCCAGTA6260.6297913438902191No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG5890.5925672548743435No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA5110.5140948510030383No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAATACTTGTCCAGTG4910.4939737218052677No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA4850.48793738304593653No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGAACCGGCCCAGGT4720.4748586490673857No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTACAGCGTCAGAAACGAGCCAGTG4230.42556188253284777No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG4120.41449526147407395No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCTTAGTA4060.4084589227147427No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA3950.39739230165596895No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAAAAATTGCCCAGTT3800.382301454757641No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG3700.3722408901587557No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT3690.3712348336988672No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTCAGTAATAGCCCAGTA3670.36922272077909013No Hit
CCCGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGGCCAAGGCCAGGG3630.365198494939536No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTTCGGCCCAGAG3530.3551379303406507No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG3480.3501076480412081No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT3060.30785327672588986No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA3040.30584116380611276No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTTAGCGTCAGGTACGGCCCAGCG3040.30584116380611276No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA2830.28471397814845373No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGTCTAGTA2710.27264130062979136No Hit
CCGGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGAAAACCCCAGCC2530.2545322843517978No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG2510.25252017143202077No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG2510.25252017143202077No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATTGTCCAGAG2460.2474898891325781No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGTT2400.24145355037324695No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG2350.23642326807380432No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA2340.23541721161391577No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGATGGGGTCCAGCG2300.23139298577436168No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA2240.22535664701503047No Hit
CCTTTTCGCTCCCCTGGCCTTCGTCTCTCAGCGTCAGGTGTCGTCCAGAG2200.22133242117547636No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTCAGCGTCAGGTAAGTCCCAGGA2070.2082536871969255No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT2070.2082536871969255No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA2040.2052355178172599No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTATCGGCCCAGCA2030.20422946135737136No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT2010.20221734843759434No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGACAGGCCCAGCC1950.19618100967826313No Hit
CCCGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGGCCAAGGCCAGGG1780.17907804986015816No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCATCGTCCAGCA1690.17002354172116138No Hit
CCCGTTCGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGTAACTGCCCAGTG1680.16901748526127286No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATCTTCGTCCAGGT1650.16599931588160727No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1630.16398720296183023No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA1600.16096903358216463No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA1600.16096903358216463No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG1600.16096903358216463No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG1540.15493269482283345No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT1530.15392663836294493No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAAATGCCCAGCA1500.15090846898327934No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG1480.1488963560635023No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGACAGGCCCAGGT1440.14487213022394818No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCGATCTCGGTCCAGGA1430.14386607376405963No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG1400.14084790438439404No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGATTCAGTGTCAGGAATAGTCCAGAG1390.13984184792450555No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA1390.13984184792450555No Hit
CCCGTTCGCTACCCATGCTGTCGCGTCTTAGCGTCAGGTACAGCCCAGCG1380.138835791464617No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCAGGTCAAGCCCAGGA1360.13682367854483993No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATCTAGGTCCAGCA1340.13481156562506286No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTCAGCGTCAGGTTGGTCCCAGGG1330.13380550916517436No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGCGTTAGTAATAGCCCAGTA1320.13279945270528581No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG1270.12776917040584318No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTGTGGACCAGAG1130.11368437996740377No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG1100.11066621058773818No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1090.10966015412784966No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTGTCGTCCAGAA1090.10966015412784966No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA1090.10966015412784966No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCTGATGCCCAGCA1080.10865409766796114No Hit
CCCGTTCGCTCCCCGTGCTGTCGCGTCTCAGCGTCTGGTGTCGTCCAGGG1070.1076480412080726No Hit
CCGGTTTGCTCCCCACGCTTTCGCCCCTGAGCGTCAGGACAGGGCCAGGA1070.1076480412080726No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTAATGGCCCAGCA1070.1076480412080726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATAACGGGCCAGAT1050.10563592828829553No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAGTTACAGTCTAGTA1030.10362381536851849No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGCGTCAGTACCAGTCCAGTT1030.10362381536851849No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1020.10261775890862995No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA1020.10261775890862995No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCT1010.10161170244874143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCT6950.0226.107274
CTTTTGC500.0226.107253
TTTTGCT700.0226.107254
TGGTTGC100.0018744884226.107253
GTTTGCT38800.0226.107254
TTCGCTG100.0018744884226.107255
TTTTCGC450.0226.107253
TGTTCGC950.0226.107253
TTTTTGC202.3194843E-7226.107253
TGTTTGC30350.0226.107253
CCTTTTT202.3194843E-7226.107251
CCTTTTG500.0226.107252
ATTCGCT5700.0226.107254
CCTTTGC400.0226.107253
CTTGCTC152.0870044E-5226.107255
ATTTGCT34350.0226.107254
ATTTGAT100.0018744884226.107254
CTGTTTG33100.0226.107252
CTTTGCT400.0226.107254
TTTGCTT100.0018744884226.107255
Produced by FastQC (version 0.11.9)