FastQCFastQC Report
Wed 30 Nov 2022
4061-JW14trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW14trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99927
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG3317433.198234711339275No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA1365113.66097250993225No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG28842.8861068580063445No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG16011.6021695837961714No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG13451.3459825672741101No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG12671.2679255856775447No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA11891.1898686040809792No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC11391.139832077416514No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG8090.8095910014310447No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA7380.7385391335675043No Hit
CAAGGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGG7070.7075164870355359No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA6420.6424690023717314No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA5800.5804237093077946No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA5520.5524032543756943No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG4530.45333093158005344No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC4440.44432435678044974No Hit
TAAGGAATATTGCGCAATGGGCGACAGCCTGACGCAGCGACGCCGCGTGG4140.4143024407817707No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG3810.38127833318322374No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG3380.33824692025178377No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG3090.30922573478639404No Hit
GGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGGGGG2940.29421477678705454No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC2900.2902118546538973No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG2810.28120527985429367No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC2760.27620162718784713No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT2760.27620162718784713No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2740.2742001661212685No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA2580.2581884775886397No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2530.2531848249221932No Hit
TCGAGAATTTTTCACAATGGGGGAAACCCTGATGGAGCGACGCCGCGTGG2520.2521840943889039No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA2490.24918190278903599No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATACCGCGTGT2490.24918190278903599No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG2250.2251643699900928No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC2230.22316290892351415No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG2220.22216217839022487No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2220.22216217839022487No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA2170.2171585257237784No Hit
TGGGGAATATTGGACAATGGGGGAAACCCTGATCCAGCAATGCCGCGTGT2070.20715122039088535No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGT2040.20414902879101743No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA2000.20014610665786023No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG1980.19814464559128162No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA1890.18913807079167794No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA1850.1851351486585207No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA1780.17813003492549562No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA1680.1681227295926026No Hit
TCGAGAATATTCGACAATGGGCGAAAGCCTGATCGAGCGACGCCGCGTGC1640.1641198074594454No Hit
TGGGGAATTTTTCGCAATGGGGGAAACCCTGACGAAGCAACGCCGCGTGG1550.1551132326598417No Hit
TAACGAATCTTCCACAATGCACGAAAGTGTGATGGAGCGACGCCGCGTGT1540.1541125021265524No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA1540.1541125021265524No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA1500.1501095799933952No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAACGCCGCGTGG1480.14810811892681658No Hit
TAAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCGACGCCGCGTGG1430.14310446626037007No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG1400.14010227466050218No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGG1400.14010227466050218No Hit
TGAGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCCACGCCGCGTGA1390.13910154412721287No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG1310.13109569986089847No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG1310.13109569986089847No Hit
TGAGGAATATTGCTCAATGGGCGCAAGCCTGAAGCAGCGACGCCGCGTGG1300.13009496932760914No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1300.13009496932760914No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1290.12909423879431986No Hit
TCGAGAATTTTTCTCAATGGGGGAAACCCTGAAGGAGCGACGCCGCGTGG1250.12509131666116263No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG1250.12509131666116263No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG1220.12208912506129475No Hit
TGAGGAATATTGCTCAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGC1200.12008766399471615No Hit
TGGGGAATCTTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGG1200.12008766399471615No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1190.11908693346142683No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA1190.11908693346142683No Hit
TCGAGAGGCTTCGGCAATGGGGGAAACCCTGACCGAGCGACGCCGCGTGG1170.11708547239484823No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC1160.11608474186155894No Hit
GGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1140.11408328079498034No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC1140.11408328079498034No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA1120.11208181972840173No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1090.10907962812853383No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA1070.10707816706195523No Hit
CAAGGAATTTTCCCCAATGGCCTAACGGCTGAGGGAGCAACGCCGCGTGG1040.10407597546208733No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGT1030.10307524492879803No Hit
CAAGGAATTTTCCCCAATGGGCGACTGCCTGAGGGAGCAACGCCGCGTGG1020.10207451439550871No Hit
TCGAGAATATTCGTCAATGCGCGAAAGCGTGAACGAGCGACGCCGCGTGC1020.10207451439550871No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG1010.10107378386221944No Hit
GGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGGAGG1000.10007305332893011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCA100.0018283083228.08
CTAGAAG202.2251334E-7228.02
TCTTGCA500.0228.08
AATTTTT1200.0228.06
GTTTCCG100.0018283083228.08
CGAAACC100.0018283083228.04
TCATTCA152.0187754E-5228.08
TTTCGAA100.0018283083228.01
TAGAAGC202.2251334E-7228.03
TCTAGAA202.2251334E-7228.01
TATTGGC100.0018283083228.08
GGGACTT152.0187754E-5228.03
ATTTTTG100.0018283083228.07
ATTTTGC2500.0228.07
TAATTCG100.0018283083228.08
CTTGCAC500.0228.09
CGAGGAT100.0018283083228.02
GAGTTTT152.0187754E-5228.05
TTTGCGC2350.0228.09
ATTGCAC2850.0228.09
Produced by FastQC (version 0.11.9)