FastQCFastQC Report
Wed 30 Nov 2022
4061-JW14trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW14trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99927
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA2078520.800184134418124No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1264012.649233940776766No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA61176.121468672130655No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA58185.822250242677154No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT45214.524302741000931No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA34393.441512303981907No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA27122.7139812062805846No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA16811.6822280264593152No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT13511.351986950473846No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC12561.2569175498113623No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA9080.9086633242266854No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG8770.8776406776947172No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG8730.8736377555615599No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGTAAATGTCCAGTA6740.674492379436989No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG5840.5844266314409519No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT3810.38127833318322374No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG3780.3782761415833559No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG3170.3172315790527085No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT3110.31122719585297265No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA2930.29321404625376524No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCATGAGCGTCAGGAAATGCCCAGAG2900.2902118546538973No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA2820.2822060103875829No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT2740.2742001661212685No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG2420.24217678905601087No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA2290.22916729212325No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2210.2211614478569356No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG2030.20314829825772815No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1990.19914537612457095No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1960.19614318452470303No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT1920.19214026239154583No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGACCAGGA1890.18913807079167794No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA1860.18613587919181002No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGTCCAGGG1860.18613587919181002No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATCGGCCCAGGG1820.18213295705865282No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG1810.1811322265253635No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA1810.1811322265253635No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT1620.1621183463928668No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA1490.14910884946010586No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTTTCAGCGTCAGTCACTGTCCAGAA1450.14510592732694866No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1420.14210373572708077No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA1360.13609935252734495No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG1350.13509862199405567No Hit
CCCATTCGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGAGATCCAGCA1340.13409789146076634No Hit
CCTTTTTGATCCCCACGCTTTCGTGATTGACCGTCAGCAAGACGCTAGAG1330.13309716092747706No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA1300.13009496932760914No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG1280.12809350826103055No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA1280.12809350826103055No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1270.12709277772774127No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1270.12709277772774127No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT1260.12609204719445194No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCG1260.12609204719445194No Hit
CCCGTTCGCTACCCTAGCTTTCGAGTCTGAGTGTCAGGATCAGACCAGGG1250.12509131666116263No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1230.12308985559458403No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG1200.12008766399471615No Hit
CCCATTCGCTACCCACGCTTTCGCTGCTCAGTGTCAGGAGTGTGCCAATC1190.11908693346142683No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA1150.11508401132826963No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG1110.11108108919511243No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA1100.11008035866182313No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1090.10907962812853383No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1080.10807889759524453No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1050.10507670599537663No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATGGCCCAGTA1040.10407597546208733No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGACAGGCCCAGGT1030.10307524492879803No Hit
CCGGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT1010.10107378386221944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA650.0226.000023
TTTTGCT600.0226.04
TTTTGAT252.5993359E-9226.04
GTTTGAT850.0226.04
CCTGCTT202.3250504E-7226.01
TGTTCGC1400.0226.03
TTTTTGC202.3250504E-7226.03
TTTTTGA252.5993359E-9226.03
TGTTTGA800.0226.03
CATTTGC16050.0226.03
GTTTACT100.0018771692226.04
CCATTTG16050.0226.02
TATTTGC100.0018771692226.03
GCTTGCT152.0909963E-5226.04
ATTTGCT16150.0226.04
TTTACTA100.0018771692226.05
CGGTTCG5850.0226.02
TTTGCTG100.0018771692226.05
TGTGTGC100.0018771692226.03
TACTACC100.0018771692226.07
Produced by FastQC (version 0.11.9)