FastQCFastQC Report
Wed 30 Nov 2022
4061-JW17trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW17trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147553
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA7388350.07217745488062No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT40752.7617195177326113No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA30532.069087039911083No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA27311.8508603688166287No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT16691.131118987753553No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG14811.0037071425182815No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG11970.8112339295032972No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA11040.7482057294667002No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT9220.6248602197176607No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA8610.5835191422743015No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG7140.48389392286161587No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC6990.4737280841460356No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC6940.47033947124084224No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA6350.4303538389595603No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA5630.3815578131247755No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC4910.33276178728999073No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA4710.31920733566921716No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA4700.31852961308817845No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG4680.31717416792610115No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA4420.2995533808190955No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA4250.288032096941438No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA4230.28667665177936064No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA3880.2629563614430069No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC3680.24940190982223337No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3670.2487241872411947No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3640.24669101949807865No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA3420.23178112271522774No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA3420.23178112271522774No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA3300.22364845174276363No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATCACAGTTTAGCA3200.21687122593237684No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA3040.206027664635758No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG3020.20467221947368064No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA2950.1999281614064099No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA2920.19789499366329386No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA2840.19247321301498443No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA2520.17078609042174678No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG2430.16468658719239865No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA2430.16468658719239865No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA2330.1579093613820119No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA2320.1572316388009732No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2310.15655391621993453No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2200.14909896782850907No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGTGTCAGACACAGTCCAGAA2140.145032632342277No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG2100.1423217420181223No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA2010.1362222387887742No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1950.13215590330254212No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1930.13080045814046476No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT1930.13080045814046476No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA1900.12876729039734874No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGAA1850.12537867749215537No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1790.12131234200592331No Hit
CACTGACGGATTTTACCGTCCACGACTCTCTGAACTCCTCAAACCAGGAA1780.12063461942488463No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG1750.11860145168176858No Hit
CCCGTTTGCTCCCCTAGCTTTCGCATCTTAGCGTCAGGAACGGCCCAGGA1670.11317967103345918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1660.1125019484524205No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACAGTCCAGGA1600.10843561296618842No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTATAGCTTAGCT1600.10843561296618842No Hit
CCCTTTCGCTCCCCACGCTTTCGTCCCTGAGCGTCAGTATCGTTCCAGCA1580.10708016780411106No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA1570.10640244522307238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG2350.0226.000022
GATCCCC8600.0226.000028
ATCCCCA8500.0226.000029
TTTGATC2850.0226.000025
TGATCCA100.0018780745226.07
TGTTTGA3150.0226.03
TTGATAC302.910383E-11226.06
GTTTGCG152.093013E-5226.04
GTTTGAT3100.0226.04
TGATACC302.910383E-11226.07
ACTGACG202.3287976E-7226.02
TTGCTGC400.0226.06
GATCCAC100.0018780745226.08
GGTTTGC650.0226.03
TGTGCTC100.0018780745226.05
TGACGGA202.3287976E-7226.04
CTGACGG202.3287976E-7226.03
TCGATGC100.0018780745226.06
GCTTGCT750.0226.04
GCTTGAT100.0018780745226.04
Produced by FastQC (version 0.11.9)