FastQCFastQC Report
Wed 30 Nov 2022
4061-JW18trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW18trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141647
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG6497045.867543964926895No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG44583.1472604432144697No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC33542.3678581261869294No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG32642.304319893820554No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG32072.264079013321849No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC27701.9555655961651146No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG24641.7395356061194376No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC19421.3710138583944593No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA18311.2926500384759296No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA18001.2707646473275114No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG14911.0526167162029552No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG12820.9050668210410386No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG11760.8302329029206407No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA9960.7031564381878896No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG7510.5301912500794228No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG7290.5146596821676421No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA7210.5090118392906309No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC7130.5033639964136197No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA6470.4567692926782777No Hit
TGGGGAATTTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA6260.4419437051261234No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA5410.3819353745578798No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA5320.3755815513212422No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG5090.35934400304983516No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4750.3353406708225377No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4670.32969282794552657No Hit
GGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGGGTG4480.31627920111262503No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC4150.292981849244954No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG3850.27180243845616214No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA3830.2703904777369094No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA3700.26121273306176623No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG3630.2562708705443814No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG3520.2485050865884911No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG3230.22803165615932564No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC3130.2209718525630617No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA3100.2188539114841825No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGA3060.2160299900456769No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG2960.20897018644941298No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG2730.19273263817800587No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG2580.18214293278360996No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA2480.175083129187346No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG2400.16943528631033483No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG2380.16802332559108205No Hit
CAAGGAATTTTCCCCAATGGCCTAACGGCTGAGGGAGCAACGCCGCGTGG2310.16308146307369728No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC2180.15390371839855416No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2130.15037381660042218No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC2030.14331401300415822No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA2020.1426080326445318No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG2020.1426080326445318No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1990.14049009156565265No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG1990.14049009156565265No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1880.13272430760976228No Hit
TGAGGAATATTGGTCAATGGTCGCAAGACTGAACCAGCCATGCCGCGTGC1820.12848842545200392No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG1780.12566450401349835No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG1760.12425254329424555No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG1720.12142862185573998No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1700.12001666113648718No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1680.1186047004172344No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1620.11436881825947602No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG1600.11295685754022322No Hit
TGAGGAATATTGCACAATAGGCGCAAGCCTGATGCAGCAACGCCGCGTGG1560.11013293610171765No Hit
TAAGGGATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC1530.10801499502283846No Hit
CGAGGAATTTTCCGCAATGGACGAATGTCTGACGGAGCAACGCCGCGTGG1520.10730901466321208No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT1510.10660303430358568No Hit
TGAGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCGACGCCGCGTGA1510.10660303430358568No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1490.10519107358433288No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA1450.10236715214582731No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC1440.10166117178620092No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1430.10095519142657451No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1420.10024921106694812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTT252.4720066E-9227.996484
GACTTTT252.4720066E-9227.996485
TTAAGAA252.4720066E-9227.996481
CAGTGAG252.4720066E-9227.996481
ATTGCAT252.4720066E-9227.996489
ACTATTG202.2285712E-7227.996466
GAGGACT202.2285712E-7227.996462
ACTGCGC100.0018291973227.996469
CATATTG152.020676E-5227.996466
GTATTGC100.0018291973227.996467
CTTCCAC100.0018291973227.996469
CATGCGC100.0018291973227.996469
TCTTCCG400.0227.996468
TCTTCCA100.0018291973227.996468
TTTCGTC202.2285712E-7227.996465
AACTTTA100.0018291973227.996468
AGGACTT100.0018291973227.996463
AGGACTA100.0018291973227.996463
TTCGTCG202.2285712E-7227.996466
GAATATA152.020676E-5227.996465
Produced by FastQC (version 0.11.9)