FastQCFastQC Report
Wed 30 Nov 2022
4061-JW18trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW18trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141647
Sequences flagged as poor quality0
Sequence length232
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA6027042.54943627468284No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT51843.6598021843032327No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA48243.4056492548377304No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA42893.027949762437609No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTTATAGCCCAGCA34982.4695192979731306No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA22711.6032813967115436No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG22501.5884558091593892No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC19551.3801916030696026No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG18201.2848842545200392No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG15851.1189788700078365No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT14691.0370851482911745No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA8080.5704321305781272No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA7940.5605484055433578No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA7170.5061879178521254No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT6620.46735899807267367No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT5730.4045267460659244No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA5520.38970115851377013No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC4850.34240047441880167No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA4790.3381645922610433No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA4740.33463469046291133No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG4540.3205150832703834No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC4500.31769116183187784No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG4370.3085134171567347No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA3430.24215126335185355No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG3390.23932734191334798No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA3300.23297351867671043No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATCGGCCAGGA3220.22732567579969923No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3180.22450175436119368No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3080.2174419507649297No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG3040.21461802932642415No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2950.20826420608978657No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA2850.20120440249352264No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATGTCGGTCCAGTA2790.19696852033576426No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA2730.19273263817800587No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGAA2730.19273263817800587No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2720.1920266578183795No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT2680.18920273637987392No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT2650.18708479530099473No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATATCGGTCCAGTA2620.18496685422211553No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT2480.175083129187346No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA2200.15531567911780694No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCG2180.15390371839855416No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA2170.15319773803892775No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA2160.15249175767930137No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG2050.144725973723411No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA1950.13766617012714705No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA1890.1334302879693887No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA1880.13272430760976228No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT1850.13060636653088312No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT1830.12919440581163033No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG1780.12566450401349835No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG1680.1186047004172344No Hit
CCCGTTCGCTACCCATGCTTTCGCGTCTTAGCGTCAGGTACAGCCCAGGG1660.11719273969798161No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTCTTGGTCCAGAA1570.11083891646134406No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACAGTCCAGGA1560.11013293610171765No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1560.11013293610171765No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATTCGTCCAGAA1490.10519107358433288No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1470.10377911286508008No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA1450.10236715214582731No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG1450.10236715214582731No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1450.10236715214582731No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA1430.10095519142657451No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTG1420.10024921106694812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGT100.0018761503226.074193
TTGATAC100.0018761503226.074196
ACTGACG202.3246503E-7226.074192
GTTTACT100.0018761503226.074194
GTGTGCG100.0018761503226.074194
GTGCTCC202.3246503E-7226.074196
GTTCGAT4150.0226.074194
CCTTTTG100.0018761503226.074192
CTGTTGG202.3246503E-7226.074192
CTGTTCG6950.0226.074192
GACGGAT202.3246503E-7226.074195
ACCGTTT100.0018761503226.074191
TTTGATC2150.0226.074195
TTTGATA100.0018761503226.074195
GCTGTTT100.0018761503226.074191
GTGCGCC100.0018761503226.074196
TGTTTGC102350.0226.074173
TGTTTGA2200.0226.074173
TTGGCTC152.0900956E-5226.074175
TTGCTAC9250.0226.074176
Produced by FastQC (version 0.11.9)