FastQCFastQC Report
Wed 30 Nov 2022
4061-JW19trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW19trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153392
Sequences flagged as poor quality0
Sequence length234
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA2560716.69383018671117No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG137398.956790445394805No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA90585.905131949514968No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG75314.909643266923959No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA55733.6331751329925943No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG44012.869119641180766No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG40522.6415979972879944No Hit
TGAGGAATATTGCGCAATGGGTGAAAGCCTGACGCAGCAACGCCGCGTGG37242.427766767497653No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG34122.224366329404402No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA30692.000756232398039No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCACGCCGCGTGA27211.7738865129863355No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC24641.6063419213518306No Hit
CAAGGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGG22831.488343590278502No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG19621.279075831855638No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA16641.0848023364973403No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA10070.6564879524355899No Hit
GGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGGGGG9230.6017262960258684No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGT8930.582168561593825No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA8140.530666527589444No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC7940.5176280379680818No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG7680.5006780014603108No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA7450.48568373839574425No Hit
TGGGGAATTGTTCGCAATGGGCGAAAGCCTGACGACGCAACGCCGCGTGG7120.4641702305204965No Hit
GGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGGGGG6510.42440283717534155No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG5950.3878950662355273No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG5940.3872431417544592No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATACCGCGTGT5740.3742046521330969No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA5580.36377386043600707No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATACCGCGTGT5570.36312193595493897No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA5530.36051423803066657No Hit
TAAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG5410.3526911442578492No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA5390.35138729529571294No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA5080.33117763638260145No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA5040.328569938458329No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG4400.28684677166996975No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA4400.28684677166996975No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA4300.28032752685928863No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC4290.2796756023782205No Hit
GGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGGAGG4240.2764159799728799No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG4110.26794096171899445No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGA3990.26011786794617714No Hit
TAACGAATCTTCCACAATGCACGAAAGTGTGATGGAGCGACGCCGCGTGT3790.24707937832481483No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA3500.22817356837383956No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGG3210.20926775842286433No Hit
TGGGGAATTTTGGACAATGGGGGCATCCCTGATCCAGCCATTCCGCGTGA3150.2053562115364556No Hit
CAAGGAATTTTCCCCAATGGGCGACTGCCTGAGGGAGCAACGCCGCGTGG3130.2040523625743194No Hit
TCGAGAATTTTTCTCAATGGGGGAAACCCTGAAGGAGCGACGCCGCGTGG3080.20079274016897883No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG3060.19948889120684263No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGA3050.1988369667257745No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC2760.1799311567747992No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG2740.17862730781266298No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2710.17667153436945862No Hit
CAAGGAATTTTCCCCAATGGCCTAACGGCTGAGGGAGCAACGCCGCGTGG2690.1753676854073224No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC2600.16950036507770938No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGAGG2550.16624074267236882No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2540.16558881819130072No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGG2490.16232919578596017No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG2460.1603734223427558No Hit
TTTCGAATCATTCACAATGGGCGAAAGCCTGATGGTGCGACGCCGCGTGA2420.15776572441848338No Hit
TCGAGAATTTTTCACAATGGGCGCAAGCCTGATGGAGCGACGCCGCGTGG2330.15189840408887034No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG2330.15189840408887034No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG2320.15124647960780224No Hit
TGAGGAATTTTTCGCAATGGGCGCAAGCCTGACGAAGCAATGCCGCGTGC2240.14603108375925733No Hit
TGGGGAATATTGGACAATGGGCGCATGCCTGATCCAGCCATGCCGCGTGA2230.14537915927818923No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCACGCCGCGTGA2210.144075310316053No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA2190.14277146135391677No Hit
TGGGGAATCTTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGG2170.14146761239178055No Hit
CAAGGAATTTTCCCCAATGGACGCAAGTCTGAGGGAGCAACGCCGCGTGG2160.14081568791071242No Hit
CAAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG2160.14081568791071242No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT2100.13690414102430376No Hit
TGGGGAATTTTTCGCAATGGGGGAAACCCTGACGAAGCAACGCCGCGTGG2060.1342964431000313No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGA1980.1290810472514864No Hit
TGGGGAATATTGGACAATGGGGGAAACCCTGATCCAGCAATGCCGCGTGT1940.12647334932721394No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG1940.12647334932721394No Hit
TCAAGAACATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC1890.12321372692187338No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC1880.12256180244080525No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA1840.1199541045165328No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1750.11408678418691978No Hit
TGGGGAATTTTGGACAATGGGGGAAACCCTGATCCAGCCATGCCGCGTGC1750.11408678418691978No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG1740.11343485970585167No Hit
CAAGGAATTTTCCCCAATGGCCGAATGGCTGAGGGAGCAACGCCGCGTGG1720.11213101074371545No Hit
TTCGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCGACGCCGCGTGG1690.11017523730051111No Hit
TAAGGAATATTGGTCAATGGACGAAAGTCTGAACCAGCCATGCCGCGTGG1640.10691561489517055No Hit
GGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGGGTG1570.10235214352769374No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1560.10170021904662564No Hit
TTAAGAATCTTGCGCAATGGAGGCAACTCTGACGCAGCGACGCCGCGTGT1540.10039637008448941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAATT1450.0228.000023
ATTTACC100.0018292598228.07
ATTTAAC100.0018292598228.07
ACTATTG152.0208767E-5228.06
GGAATAG152.0208767E-5228.04
AATTTTA100.0018292598228.06
ATTAGTT100.0018292598228.09
AGTTTCC252.4720066E-9228.07
TTTACGC100.0018292598228.09
CTTCCCC100.0018292598228.09
TCATTCA152.0208767E-5228.08
TCTTCCC100.0018292598228.08
TTTAACC100.0018292598228.08
GAGTATT152.0208767E-5228.05
TGTGGAC100.0018292598228.09
GGATCTT500.0228.05
ATTGTTC1200.0228.07
GATTTTG100.0018292598228.06
ATTGTAC202.2290078E-7228.09
CAATTTT100.0018292598228.05
Produced by FastQC (version 0.11.9)