FastQCFastQC Report
Wed 30 Nov 2022
4061-JW19trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW19trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153392
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1582810.318660686346094No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT145819.505710858454156No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA110087.176384687597789No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA96566.2949827891937No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG85445.570042766245958No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG44292.8873735266506726No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTTGGCCAGAA37772.4623187649942633No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA35702.3273703974131634No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT29111.8977521643892774No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATGTCGGCCCGGCG26541.730207572754772No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA25511.6630593512047562No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT22691.4792166475435484No Hit
CCGGTTCGCTCCCCACGCTTTCGCCCCTCAGCGTCAGGTCAGGCCCAGGT22661.4772608741003441No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA21781.4198915197663502No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT20651.3462240534056535No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGC13660.8905288411390424No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT13280.8657557108584543No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG11650.7594920204443517No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG9970.6499687076249088No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG7560.4928549076874935No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT7240.47199332429331387No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGGA7230.47134139981224576No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTTGGCCCAGAT6600.4302701575049546No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA6310.4113643475539793No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG5680.37029310524668824No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG5550.36181808699280277No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGCCTGAGCGTCAGTTCCGGGCCAGAA4950.322702618128716No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT4840.31553144883696677No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC4690.30575258162094504No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT4560.29727756336705957No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA3680.2399082090330656No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG3610.2353447376655888No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG3510.2288254928549077No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG3370.2196985501199541No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA3290.21448315427140918No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA3130.2040523625743194No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTTAGTAATAGCCCAGTA3090.20144466465004696No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG3090.20144466465004696No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT3030.19753311776363824No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG2950.19231772191509336No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAGCGTCAGTGTTATCCCAGGA2940.19166579743402523No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG2840.18514655262334412No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTCTCTGGCCAGGG2830.184494628142276No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCGGTATCGCGCCAGAT2810.18319077918013976No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAATCGACGGCCAGGT2740.17862730781266298No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG2510.16363304474809637No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGA2470.16102534682382394No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT2450.1597214978616877No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA2450.1597214978616877No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTCTTGGTCCAGAA2340.15255032856993847No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA2330.15189840408887034No Hit
CCTATTCGCTCCCCCAGCTTTCGTTTCTCAGTGTCAGTCTCGGCCCAGTA2310.15059455512673411No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2310.15059455512673411No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGT2200.14342338583498487No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATAACGGGCCAGAT2190.14277146135391677No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGGT2180.14211953687284865No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGTTGCAGGCCAGTT2160.14081568791071242No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG2120.13820798998643996No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA2060.1342964431000313No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC2040.13299259413789508No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGCATGTTCCAGAG1990.1297329717325545No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA1970.12842912277041826No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA1960.12777719828935016No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1900.12386565140294148No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGAATAGTCCAGAG1870.12190987795973715No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA1840.1199541045165328No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG1820.11865025555439657No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGTGTCAGACATCGTCCAGAA1720.11213101074371545No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1710.11147908626264734No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTCTCCGTCCAGCA1650.10756753937623866No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCA1640.10691561489517055No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT1630.10626369041410243No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA1540.10039637008448941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC1100.0226.000023
TGTTTGA1500.0226.03
GTTTGAT1700.0226.04
TTGGCTC202.3290886E-7226.05
TCATTTG100.0018781464226.02
TTTGTTA100.0018781464226.05
TTGCTCG100.0018781464226.06
CGGTTTG950.0226.02
GATCCAC100.0018781464226.08
TGTGCTC202.3290886E-7226.05
TCGATCC3200.0226.06
GTTCGAT3200.0226.04
TATTCGC302.910383E-11226.03
TGTTCGA3100.0226.03
CTGTTGG152.0931728E-5226.02
CTGTTCG4200.0226.02
ACTGTTT100.0018781464226.01
TGTTACC100.0018781464226.07
CTATTTG302.910383E-11226.02
CTATTCG302.910383E-11226.02
Produced by FastQC (version 0.11.9)