FastQCFastQC Report
Wed 30 Nov 2022
4061-JW20trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW20trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109594
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA2590123.633593079913133No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA1519013.860247823785974No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA101549.265105753964633No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC35873.272989397229775No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT23342.1296786320419003No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT10130.9243206744894794No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA8430.7692026935781155No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG5450.49728999762760734No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA5110.4662664014453346No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA5020.45805427304414476No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA4860.4434549336642517No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGTGTCAGTTCAGACCCAGAT4460.406956585214519No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA3720.33943464058251366No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA3560.3248353012026206No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA3300.30111137471029437No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT3180.2901618701753746No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2870.2618756501268317No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG2690.24545139332445204No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTTGGCCAGAA2590.2363268062120189No Hit
CCTGTTTGCTCCCCTGGCTTTCGAATCTCAGTGTCAGGATCAGTCCAGAA2530.23085205394455904No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGTGTCAGAAACAGTCCAGGA2370.21625271456466597No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAATATTGGCTTTGTG2230.2034782926072595No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA2180.19891599905104293No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA2120.19344124678358304No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2030.1852291183823932No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA1980.18066682482617663No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGAT1910.1742796138474734No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1880.17154223771374344No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1860.16971732029125683No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA1850.1688048615800135No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA1810.16515502673504023No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA1740.15876781575633703No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA1720.15694289833385042No Hit
CCGGTTCGTGCCCCCAGCTTTCGTCCCTCACCGTCGGACCCGTTCTGGTG1710.15603043962260707No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG1700.15511798091136375No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT1560.14234355895395734No Hit
CCTGTTTGCTCCCCTAGCTTTCGTCCATGAGCGTCAGTTATAGGCTCGGA1530.13960618282022738No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA1500.13686880668649742No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA1500.13686880668649742No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1460.13321897184152418No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1420.1295691369965509No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG1390.12683176086282097No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA1370.1250068434403343No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG1300.11861963246163111No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG1300.11861963246163111No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA1300.11861963246163111No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA1290.11770717375038779No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA1220.11131996277168457No Hit
CCCATTCGCTACCCTAGCTTTCGCGTCTTAGCGTCAGAAACGGTCCAGAA1220.11131996277168457No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1170.10675766921546799No Hit
CCTGTTTGCTCCCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA1120.10219537565925142No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1120.10219537565925142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC1950.0225.996835
TATTTGC100.0018774954225.996813
TGTTTGA2100.0225.996813
GTTTGCG202.3262328E-7225.996814
GTTTGCA100.0018774954225.996814
CTTTTGC100.0018774954225.996813
CGATTTG100.0018774954225.996812
ATTCGAT100.0018774954225.996814
GCTTGCT600.0225.996814
TTTTTGC202.3262328E-7225.996813
TGTCTGC202.3262328E-7225.996813
GTTCGAT1100.0225.996814
CGGATCG100.0018774954225.996812
TGTTCGC2200.0225.996813
CTGTTTG81800.0225.996812
ACTGTTT100.0018774954225.996811
TTTGCTC79950.0225.996815
GGTTGCT350.0225.996814
TTTGATG152.0916641E-5225.996815
TTTTGCT400.0225.996814
Produced by FastQC (version 0.11.9)