FastQCFastQC Report
Wed 30 Nov 2022
4061-JW23trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW23trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131570
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4775536.29626814623394No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG59804.545109067416584No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC56044.25932963441514No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG34472.619898153074409No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG18451.4022953560842137No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA16731.2715664665197235No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC14381.0929543208938208No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG12610.9584251729117581No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG11750.8930607281295128No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG11470.8717792809911075No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA11200.8512578855362165No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG10940.8314965417648401No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA9330.7091282207190088No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA8410.6392034658356768No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA8340.6338831040510755No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC8090.6148818119632136No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC6170.4689518887284336No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA5570.42334878771756485No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG5330.40510754731321724No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC5290.4020673405791594No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT5230.39750703047807245No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG5020.3815459451242684No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4910.3731853766056092No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC4810.3655848597704644No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4620.3511438777836893No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA4560.3465835676826024No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA4460.3389830508474576No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG4070.30934103519039297No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG3940.2994603633047047No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA3890.29566010488713235No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG3620.2751387094322414No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC3390.2576575207114084No Hit
GGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGGGTG3380.2568974690278939No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG3290.2500570038762636No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA3210.24397659040814776No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG3020.22953560842137263No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGG2910.2211750399027134No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC2760.2097742646499962No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA2540.19305312761267768No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2500.19001292087861976No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG2500.19001292087861976No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG2470.1877327658280763No Hit
GCGCGAAAACTTTACAATGCCGGCAACGGCGATAAGGGGACCTCGAGTGC2440.1854526107775329No Hit
TGGGGAATCTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG2430.18469255909401838No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2270.17253173215778672No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC2240.17025157710724328No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAATGCCGCGTGC2170.16493121532264193No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC2170.16493121532264193No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG2140.1626510602720985No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA2110.16037090522155506No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG2110.16037090522155506No Hit
TAGGGAATTTTCGGCAATGGGGGGAACCCTGACCGAGCGATGCCGCGTGA2110.16037090522155506No Hit
TGAGGAATATTGCGCAATGGACGAATGTCTGACGCAGCAACGCCGCGTGG2100.1596108535380406No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC2040.1550505434369537No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT1970.14973018165235236No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG1940.14745002660180892No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG1920.14592992323477996No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA1790.13604925134909174No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC1710.1299688378809759No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1700.12920878619746143No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1690.12844873451394695No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1670.126928631146918No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1640.12464847609637456No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCAACGCCGCGTGG1640.12464847609637456No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1630.12388842441286008No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1610.1223683210458311No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC1600.12160826936231664No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1590.12084821767880216No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC1550.11780801094474425No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1540.11704795926122977No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA1490.11324770084365737No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1470.1117275974766284No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1450.11020749410959946No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1410.10716728737554153No Hit
TGGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGA1400.10640723569202706No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1360.10336702895796913No Hit
TGGGGAATATTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA1360.10336702895796913No Hit
TAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA1350.10260697727445467No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1350.10260697727445467No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGA1320.10032682222391122No Hit
CTAAGAATCTTCCGCAATGGGGGAAACCCTGACGGAGCGACGCCGCGTGG1320.10032682222391122No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG1320.10032682222391122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGAC350.0228.000029
ATTAGTA100.0018289653228.09
AGGAGTA202.2278073E-7228.03
CTTCCCC100.0018289653228.09
CTTCCAC152.020226E-5228.09
TCATTCG100.0018289653228.08
TCTTCCC100.0018289653228.08
AACTTTA550.0228.08
GCTATTG100.0018289653228.06
ATTGTTC252.4701876E-9228.07
ACTTCAC152.020226E-5228.09
TTTCCTC100.0018289653228.09
TTTCCAC252.4701876E-9228.09
TAACGAA152.020226E-5228.01
CATTCGC100.0018289653228.09
AATATTA302.7284841E-11228.06
TATTGCC152.020226E-5228.08
AACATTG152.020226E-5228.06
AATTGTT252.4701876E-9228.06
GAGAATC800.0228.03
Produced by FastQC (version 0.11.9)