FastQCFastQC Report
Wed 30 Nov 2022
4061-JW23trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW23trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131570
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA4809636.55544577031238No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA68755.225355324162043No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT38402.9185984646955996No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA30062.284715360644524No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG15701.193281143117732No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT14971.137797370221175No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA11970.9097818651668313No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA11910.9052215550657444No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA11670.886980314661397No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT11510.8748194877251653No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC8380.6369233107851334No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA8140.6186820703807859No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA7830.5951204681918371No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG7560.5745990727369461No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT6350.48263281903169414No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGGCCAGTG5700.43322945960325304No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC5430.41270806414836203No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA4750.3610245496693775No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA4600.34962377441666037No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA4600.34962377441666037No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA4540.34506346431557344No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA4310.32758227559474046No Hit
CCGGTTCGTGCCCCTAGCTTTCGTCCCTTGCCGTCAGGTCCGTTCTGGTG4100.3116211902409364No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTGTGGTCCAGC4040.3070608801398495No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG3710.28197917458387173No Hit
CCTGTTTGCTCCCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA3470.2637379341795242No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCAAGACCCAGAA3440.26145777912898077No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3430.26069772744546627No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3420.2599376757619518No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA3410.25917762407843736No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA3100.2356160218894885No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3100.2356160218894885No Hit
CACTGACGGATTTTACCGTCCACGACTCTCTGAACTCCTCAAACCAGGAA2930.22269514326974235No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA2880.21889488485216996No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA2700.20521395454890934No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA2700.20521395454890934No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA2540.19305312761267768No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2530.19229307592916317No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG2490.18925286919510528No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA2430.18469255909401838No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2420.1839325074105039No Hit
CCCATTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAATGGTCCAGGA2400.18241240404347497No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA2350.17861214562590258No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA2320.17633199057535912No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATCACGGCCCGGCG2240.17025157710724328No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT2240.17025157710724328No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA2210.16797142205669985No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCAGTCCAGGA2190.1664513186896709No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT2110.16037090522155506No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCCGGCCCAGTA2050.1558105951204682No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA2050.1558105951204682No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1990.15125028501938131No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG1970.14973018165235236No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG1950.1482100782853234No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1910.14516987155126548No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA1880.14288971650072205No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG1880.14288971650072205No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG1840.13984950976666413No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTTGGCCAGGT1800.13680930303260622No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1790.13604925134909174No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1780.13528919966557726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1770.13452914798206278No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC1740.13224899293151934No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1720.13072888956449039No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGTTTCAGCGTCAGTAGCAGACCAGAA1690.12844873451394695No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCTGGCCAGGT1630.12388842441286008No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCAATAATGGCTTTGTG1500.11400775252717185No Hit
CCCGTTCGCTCCCCATGCTGTCGCGTCTGAGCGTCAGAGTAGGCCCAGCA1460.11096754579311392No Hit
CCTGTTTGCTACCCACGCTTTCGAACATGAGTGTCAGTTACATGCCAGGA1450.11020749410959946No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA1430.1086873907425705No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1390.10564718400851258No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1380.1048871323249981No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGGA1350.10260697727445467No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1340.10184692559094019No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTTATTGGCCAGGA1330.10108687390742571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT2900.0226.000024
TGTTCGA2850.0226.000023
GACGGAT550.0226.000025
TTTGATC2200.0226.000025
GGTTCGT550.0226.000023
TGTTTGA2150.0226.03
GTTTGCT98550.0226.04
GTTTGAT2250.0226.04
TTGGCTC152.0924985E-5226.05
GTTTACT100.0018778435226.04
GGTTTGC400.0226.03
TGGTTGC202.3278335E-7226.03
GTTCACT100.0018778435226.04
TTTGCTG302.910383E-11226.05
GGTTGCT202.3278335E-7226.04
GGTTCGC2500.0226.03
CGTTTTG152.0924985E-5226.02
TTCGATG100.0018778435226.05
TGTGTGC100.0018778435226.03
GTTGGCT152.0924985E-5226.04
Produced by FastQC (version 0.11.9)