FastQCFastQC Report
Wed 30 Nov 2022
4061-JW25trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW25trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128436
Sequences flagged as poor quality0
Sequence length234
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA1544312.023887383599615No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG111008.642436699990656No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG71345.5545174250210225No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA49163.827587280824691No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA44183.439845526176461No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA41653.2428602572487466No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG34162.659690429474602No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC24061.8733065495655423No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA20931.629605406583824No Hit
CAAGGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGG18541.4435205082687097No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG15761.2270702918184933No Hit
CAAGGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGG15361.1959263757824907No Hit
TAACGAATCTTCCACAATGCACGAAAGTGTGATGGAGCGACGCCGCGTGT12780.9950481173502757No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG12370.9631256034133732No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG12200.9498894390980722No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA10940.8517861035846647No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA9550.7435609953595566No Hit
TGAGGAATATTGCGCAATGGGTGAAAGCCTGACGCAGCAACGCCGCGTGG9520.7412252016568563No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAACGCCGCGTGG9520.7412252016568563No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAACGCCGCGTGG9350.7279890373415554No Hit
CAAGGAATTTTCCCCAATGGGCGAGAGCCTGAGGGAGCAACGCCGCGTGG9220.7178672646298545No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA9060.7054096982154536No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA8750.6812731632875517No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA8590.6688155968731508No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC8540.6649226073686505No Hit
CAAGGGATTTTCCCCAATGGCCTAACGGCTGAGGGAGCAACGTCGCGTGG7920.6166495375128468No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA7640.5948487962876452No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA7610.592513002584945No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG7220.5621476844498428No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA6880.5356753558192406No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG6540.5092030271886386No Hit
TAAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG6170.48039490485533654No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA6070.47260892584633596No Hit
CAAGGAATTTTCCCCAATGGACGCAAGTCTGAGGGAGCAACGCCGCGTGG5730.4461365972157339No Hit
TTAAGAATCTTGCGCAATGGAGGCAACTCTGACGCAGCGACGCCGCGTGT5720.4453579993148339No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATACCGCGTGT5620.43757202030583325No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC5000.3892989504500296No Hit
GGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGGGGG4800.3737269924320284No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG4200.3270111183780248No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG4120.3207823351708244No Hit
TTTCGAATCATTCACAATGGGCGAAAGCCTGATGGTGCGACGCCGCGTGA4110.3200037372699243No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATACCGCGTGT4000.31143916036002367No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGA3910.3044317792519231No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGT3760.29275281073842224No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCACGCCGCGTGA3640.28340963592762153No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG3620.28185244012582145No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGA3440.2678376779096203No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCACGCCGCGTGA3440.2678376779096203No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG3360.2616088947024199No Hit
TCGAGAATTTTTCTCAATGGGCGAAAGCCTGAAGGAGCGACGCCGCGTGG3260.2538229156934193No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG3240.25226571989161917No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC3230.2514871219907191No Hit
CAAGGAATTTTCCCCAATGGGCTCACGCCTGAGGGAGCAACGTCGCGTGG3180.2475941324862188No Hit
CAAGGAATTTTCCCCAATGGCCTAACGGCTGAGGGAGCAACGCCGCGTGG3060.2382509576754181No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA3060.2382509576754181No Hit
GGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGGAGG2990.2328007723691177No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCACGCCGCGTGA2800.21800741225201656No Hit
TCGAGAATTTTTCACAATGGGCGCAAGCCTGATGGAGCGACGCCGCGTGG2730.21255722694571616No Hit
TCGAGAATTTTTCTCAATGGGGGAAACCCTGAAGGAGCGACGCCGCGTGG2710.21100003114391605No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA2430.18919928991871438No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG2390.18608489831511416No Hit
TCGAGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCGACGCCGCGTGG2340.18219190881061384No Hit
CAAGGAATTTTCCCCAATGGGCGCATGCCTGAGGGAGCAACGCCGCGTGG2340.18219190881061384No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2260.17596312560341337No Hit
TGGGGAATCTTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGG2230.1736273319007132No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGA2150.16739854869351273No Hit
GGAATTTTCGGCAATGGGGGAAACCCTGACCGAGCAACGCCGCGTGGGCG2140.16661995079261266No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGT2120.16506275499081255No Hit
TGGGGAATTTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA1950.15182659067551155No Hit
TGGGGAATTTTGGACAATGGGGGCATCCCTGATCCAGCCATTCCGCGTGA1940.15104799277461148No Hit
TCGAGAATCTTCGGCAATGGGCGCAAGCCTGACCGAGCGACGCCGCGTGC1940.15104799277461148No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC1920.14949079697281134No Hit
TGCTGAATTATTCGCCAATGCGCGAAAGCGTGACGGTGCGACGCCGCGTG1910.1487121990719113No Hit
TGAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCCATGCCGCGTGA1870.14559780746831108No Hit
TAAGGAATATTGCGCAATGGGCGACAGCCTGACGCAGCGACGCCGCGTGG1850.14404061166651094No Hit
TGGGGAATATTGGACAATGGGGGAAACCCTGATCCAGCAATGCCGCGTGT1800.14014762216201065No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1760.1370332305584104No Hit
TCGAGAATCTTTCACAATGGGGGCAACCCTGATGGAGCGACGCCGCGTGG1740.1354760347566103No Hit
GGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGGGGG1740.1354760347566103No Hit
TAGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA1690.13158304525211No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGA1660.1292472515494098No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCTCGTGA1660.1292472515494098No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC1640.1276900557476097No Hit
TGGGGAATATTGGACAATGGGCGCATGCCTGATCCAGCCATGCCGCGTGA1630.12691145784670965No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA1500.11678968513500888No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAAGACCGCGTGA1470.11445389143230869No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGAGG1450.11289669563050858No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGAGGG1440.11211809772960853No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1410.10978230402690833No Hit
TCTAGAAGCTTCGGCAATGGGGGAAACCCTGACCGAGCGACACCGCGTGA1410.10978230402690833No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1390.10822510822510822No Hit
GGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGGGTG1350.10511071662150798No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1320.10277492291880781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTG152.0201134E-5228.06
TCTTGCT100.0018289143228.08
CTTCCAC1200.0228.09
TCTTCCA1200.0228.08
GAGTATT400.0228.05
GGACTAT152.0201134E-5228.04
ATTGTTC202.227589E-7228.07
TTTCCCA100.0018289143228.09
TAACGAA1350.0228.01
CATTCAC400.0228.09
TCTAGAG302.7284841E-11228.01
AATATTA100.0018289143228.06
CTTGGTC152.0201134E-5228.09
TATTGCC152.0201134E-5228.08
ATTTTTC1950.0228.07
GCTTCGA100.0018289143228.08
TAATTGT100.0018289143228.08
CTTGCTC100.0018289143228.09
CTTGCAC1150.0228.09
TATTCGC152.0201134E-5228.09
Produced by FastQC (version 0.11.9)