FastQCFastQC Report
Wed 30 Nov 2022
4061-JW25trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW25trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128436
Sequences flagged as poor quality0
Sequence length232
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1485011.56217882836588No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT102747.999314833847208No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA87136.783923510542215No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG41023.193808589492043No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGC38973.034196019807531No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG20021.5587529976019185No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGCGTCAGACACAGCCCAGAT19451.514372917250615No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGTGTCAGGTCAGGCCCAGGT18511.4411847145660095No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATGTCGGCCCGGCG17321.3485315643589024No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA17251.3430813790526022No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATCAGCGTCAGAACCGGTCCAGGT16951.3197234420256003No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT15131.1780186240617896No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT14941.1632252639446883No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT13931.0845868759537824No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTCATTCCCAGCA13181.026192033386278No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG11920.9280886978728706No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAATCGACGGCCAGGT11470.8930517923323679No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA11350.8837086175215672No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTCTCGACCAGAA10070.7840480862063596No Hit
CCGGTTTGCTCCCCACGCTGTCGCGTCTCAGTGTCAGAACAGGGCCAGGT10050.7824908904045595No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG9920.7723691176928587No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTTGGCCAGAA9690.7544613659721574No Hit
CCGGTTCGCTCCCCACGCTGTCGCACCTCAGCGTCAGGTCAGGCCCAGGT9320.7256532436388552No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA9160.7131956772244542No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTCAGTGTCAGTAATAGCCCAGTA8890.6921735339001526No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA7180.5590332928462425No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG6990.5442399327291413No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT6330.4928524712697374No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG6290.4897380796661372No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGAT6160.4796163069544364No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG5970.4648229468373353No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTTAGTGTCAGGTCAGGCCCAGGT5480.42667164969323246No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCGGTATCGCGCCAGAT5290.41187828957613126No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG5230.4072067021707309No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTCTCCGTCCAGCA5030.3916347441527298No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATAACGGCCCGGCG4390.34180447849512596No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG4320.33635429318882554No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG3620.28185244012582145No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGCAATTTAGTA3480.2709520695132206No Hit
CCGGTTCGCTCCCCACGCTTTCGCCCCTCAGCGTCAGGTCAGGCCCAGGT3410.26550188420692017No Hit
CCTGTTTGCTCCCCACGCTTTCGTAGTTGAGTGTCAGTATCTGTCCAGGT3400.2647232863060201No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGTGTCAGACATCGTCCAGAA3210.249929926188919No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA2830.22034320595471674No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT2810.21878601015291663No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG2790.21722881435111652No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTCTGGTCCAGTG2600.2024354542340154No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG2580.20087825843221524No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG2540.19776386682861502No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTGTCCAGGG2540.19776386682861502No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG2510.19542807312591484No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA2440.18997788781961444No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC2420.1884206920178143No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTGCCGTCCAGGT2390.18608489831511416No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT2390.18608489831511416No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGT2370.18452770251331405No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTTGGCCCAGAT2350.1829705067115139No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGAT2330.1814133109097138No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT2320.18063471300881373No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG2240.17440592980161324No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2200.17129153819801302No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG2200.17129153819801302No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG2160.16817714659441277No Hit
CCCTTTGGCTACCCACGCTTTCGTGCCTGAGTGTCAGCAATTGTCCAGGA2120.16506275499081255No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATGTCGGCCCGGTG2110.16428415708991248No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGAGCCAGTG2020.15727677598181194No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGAAGAGGTCCAGCA2010.1564981780809119No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATGAGCGTCAGAAATGCCCCAGCA1980.1541623843782117No Hit
CCTGTTTGCTACCCTAGCCTTCGCGCCTCAGCGTCAGATACAGCCCAGAT1950.15182659067551155No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA1830.14248341586471083No Hit
CCCGTTCGCTCCCCATGCTGTCGTGTCTGAGCGTCAGAACTGGCCCAGCG1810.14092622006291072No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGACAGGCCCAGGT1790.13936902426111059No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG1780.13859042636021052No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC1750.13625463265751037No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG1730.13469743685571023No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC1730.13469743685571023No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGCGTCAGTACCTGTCCAGGT1720.13391883895481016No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCTACGGCCCAGCA1700.13236164315301005No Hit
CCCCTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1630.12691145784670965No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTTATGGACCAGAG1540.1199040767386091No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCAGGCCAGAC1510.11756828303590894No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGACCAGGA1480.11523248933320875No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG1440.11211809772960853No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGAA1440.11211809772960853No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1420.11056090192780839No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGCATGTTCCAGAG1410.10978230402690833No Hit
CCCGTTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA1370.10666791242330811No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1370.10666791242330811No Hit
CCGTTTTGCGCCCCTAGCCTTCATCCCTTACTGTCAGAACTGTCCTCGTA1300.1012177271170077No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG1300.1012177271170077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTTG63300.0226.000022
CGTTTGC6650.0226.000023
CTGCTCG252.6029738E-9226.02
TGTTTGA3450.0226.03
GTTTGCG202.3276152E-7226.04
GTTTGAT3500.0226.04
TTGGCTC100.0018777912226.05
TTGGCTA152.092382E-5226.05
TTGCTCG100.0018777912226.06
TTGCTAC29350.0226.06
CCTTTTG350.0226.02
CTGTTTT100.0018777912226.02
CTGTTGG152.092382E-5226.02
CTTTGCT202.3276152E-7226.04
TTTGATC3500.0226.05
TTTTGCT350.0226.04
GCTCGAT202.3276152E-7226.04
CATTTGC17450.0226.03
ATTTGCT17550.0226.04
TGGCTAC152.092382E-5226.06
Produced by FastQC (version 0.11.9)