FastQCFastQC Report
Wed 30 Nov 2022
4061-JW26trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW26trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91692
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4375947.72390175805959No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG17411.8987479823757796No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG14401.5704750687082842No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC14221.5508441303494307No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC13221.4417833616891331No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA12251.3359944160886446No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG11521.2563800549666275No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA6480.7067137809187279No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG6460.7045325655455219No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA5510.6009248353182393No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG4960.5409414125550757No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC4520.49295467434454476No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4000.43624307464119005No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT3960.43188064389477815No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA3810.41552152859573355No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG3530.3849845133708502No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG3150.3435414212799372No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA3020.32936352135409847No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2880.3140950137416569No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2840.3097325829952449No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA2800.30537015224883307No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG2710.2955546830694063No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC2530.27592374471055275No Hit
GGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2440.2661082755311259No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG2350.25629280635169915No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA2190.23884308336605156No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2160.23557126030624262No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG2100.2290276141866248No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1960.21375910657418312No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1940.2115778912009772No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1880.2050342450813593No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA1830.19958120664834447No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA1820.19849059896174148No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG1770.19303756052872661No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC1760.19194695284212362No Hit
AGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1740.1897657374689177No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG1680.18322209134929984No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1660.18104087597609386No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG1640.17885966060288794No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1560.17013479911006413No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA1480.16140993761724032No Hit
TGAGGACTATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1360.14832264537800463No Hit
TAGGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG1330.1450508223181957No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGGGG1290.1406883915717838No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1270.13850717619857783No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA1230.13414474545216595No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1150.12541988395934214No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1130.12323866858613619No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG1030.11233259172010643No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG1020.11124198403350348No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCATCAACGCCGCGTGG1020.11124198403350348No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAATGCCGCGTGC1010.11015137634690049No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC1010.11015137634690049No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG1010.11015137634690049No Hit
TAAGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCCATGCCGCGTGA970.1057889456004886No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG950.10360773022728265No Hit
CGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG940.10251712254067966No Hit
TGAGGAATATTGCGCAATGGGCGAAAGTCTGACGCAGCAACGCCGCGTGG930.10142651485407671No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG920.10033590716747372No Hit
TGAGGAATATTGCGCAATGGGCGCAAGCCTGACGCAGCAACGCCGCGTGG920.10033590716747372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTG650.0227.9886
AGGACTA450.0227.9883
ATTGAGC550.0227.9889
AATCATG100.001828351227.987996
CATTGCA100.001828351227.987998
CATTGAG100.001828351227.987998
ACATTGC100.001828351227.987997
ACATTCC152.018657E-5227.987997
ATTGTTC100.001828351227.987997
TTTCCAC100.001828351227.987999
TAACGAA100.001828351227.987991
CATTCCA152.018657E-5227.987998
AATATTA100.001828351227.987996
GACTTTT100.001828351227.987995
ATCGTGC100.001828351227.987997
AATATAA100.001828351227.987996
CTTGGAC100.001828351227.987999
TATTGGA750.0227.987998
AACATTG202.2247332E-7227.987996
AATTGTT100.001828351227.987996
Produced by FastQC (version 0.11.9)