FastQCFastQC Report
Wed 30 Nov 2022
4061-JW27trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW27trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143557
Sequences flagged as poor quality0
Sequence length234
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG5831940.62428164422494No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG70524.912334473414741No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC49663.459253118970165No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG31052.162903933629151No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG22471.5652319287809024No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA18681.3012252972686809No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA18581.2942594230863003No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG17861.2441051289731604No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC17841.2427119541366844No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG13680.9529315881496547No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC12060.8400844263950904No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA10560.7355963136593826No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG9460.658971697653197No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA8740.6088174035400572No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG7460.5196542140055866No Hit
CGAGGAATTTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGG7130.4966668292037309No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG6710.46741015763773275No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT6370.443726185417639No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA5920.4123797515969267No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG5590.38939236679507094No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG5580.38869577937683286No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA5440.37894355552150016No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA5400.376157205848548No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA5370.3740674435938338No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC5230.36431521973850106No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC5160.3594391078108347No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG5150.3587425203925967No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC4780.3329687859177888No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGGGGG4210.2932633030782198No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4180.29117354082350566No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG3880.2702759182763641No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA3440.23962607187388982No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG3410.23753630961917566No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC3300.2298738480185571No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT3240.2256943235091288No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG3120.21733527449027215No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA3040.21176257514436775No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG2870.19992058903432083No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2710.18877519034251203No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2690.18738201550603592No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG2660.18529225325132176No Hit
TGAGGAATATTGCGCAATGGACGAATGTCTGACGCAGCAACGCCGCGTGG2660.18529225325132176No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG2550.1776297916507032No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2530.1762366168142271No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG2490.1734502671412749No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA2400.16718098037713242No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG2250.15673216910356166No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA2240.15603558168532358No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC2230.15533899426708556No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG2220.1546424068488475No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2110.1469799452482289No Hit
TGGGGAATCTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG2080.14489018299351478No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC2060.14349700815703867No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2000.13931748364761035No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1940.13513795913818205No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA1930.134441371719944No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAATGCCGCGTGC1910.1330481968834679No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1810.12608232270108738No Hit
CTAAGAATCTTCCGCAATGGGGGAAACCCTGACGGAGCGACGCCGCGTGG1720.11981303593694491No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1700.1184198611004688No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG1670.11633009884575464No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC1660.1156335114275166No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC1620.1128471617545644No Hit
TGAGGAATATTGCTCAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGC1610.11215057433632633No Hit
TAAGGAATATTGGGCAATGGACGCAAGTCTGACCCAGCCATGCCGCGTGC1580.11006081208161217No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1570.10936422466337413No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1530.10657787499042193No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1480.10309493789923167No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG1440.10030858822627946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA100.0018291385228.03
TAGAGGC100.0018291385228.03
ATTAGAC100.0018291385228.09
AACTTTA350.0228.08
TTTCCAC100.0018291385228.09
AATATAG100.0018291385228.06
TAGCAAT100.0018291385228.05
ATATAGC202.2284985E-7228.07
TTAAGAA202.2284985E-7228.01
TATTCCC100.0018291385228.08
GATATAG100.0018291385228.06
GGTATAT100.0018291385228.04
TATTAGA100.0018291385228.08
AGATTGC100.0018291385228.07
CAGTGAG400.0228.01
GTATATT100.0018291385228.05
TCGAGTA100.0018291385228.06
ATTGCTC550.0228.09
ACTTTAC350.0228.09
TATCGCG100.0018291385228.08
Produced by FastQC (version 0.11.9)