FastQCFastQC Report
Wed 30 Nov 2022
4061-JW27trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW27trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143557
Sequences flagged as poor quality0
Sequence length232
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA4494031.30463857561805No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACTCGACCAGAA1728412.039816936826488No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGCTCGACCAGAA42632.9695521639488147No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT35932.50283859372932No Hit
CCCATTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA26991.8800894418245018No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTACCGGTCCAGGG19371.3492898291271063No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCGCCAGAT18111.2615198144291118No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT17871.2448017163913985No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGTCCAGTA16751.1667839255487367No Hit
CCTGTTTGCTCCCCACGCTTTCACGTCTCAGCGTCAGTATCGAGCCAGAG13370.9313373781842752No Hit
CCCATTCGCTCCCCACGCTTTCGCAGCTCAGCGTCAGAACAGCGCCAATC12460.8679479231246126No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAACGATCCAGAA12130.8449605383227569No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGA8890.619266214813628No Hit
CCTGTTTGCTCCCCTAGCCTTCGCGCCTCAGTGTCAGTTACAGGCCAGCT8190.5705050955369644No Hit
CCCGTTCGCTCCCCGTGCTTTCGCGTCTGAGCGTCAGGTCAGGGCCAGTC6980.4862180179301601No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAAGA5920.4123797515969267No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCCAGCCAGAA5330.3712810939208816No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTGCGACCCAGGA5150.3587425203925967No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGAAATGGTCCAGGA3980.27724179245874464No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTCAGGCCCAGCA3970.27654520504050656No Hit
CCTGTTTGCTACCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA3910.27236568053107824No Hit
CCTGTTTGCTCCCCACGCTTTCACGCCTCAGCGTCAGTATCGAGCCAGAG3890.27097250569460213No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA3790.26400663151222165No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGGA3680.2563441699116031No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGCCCAGGA3540.24659194605627036No Hit
CACTGACGGATTTTACCGTCCACGACTCTCTGAACTCCTCAAACCAGGAA3530.24589535863803225No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTCAGCACCAGAT3230.22499773609089072No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC3230.22499773609089072No Hit
CCCGTTCGCTCCCCTAGCTTTCGTGTCTGAGCGTCAGATGCAACCCAGGA3190.2222113864179385No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGCA3180.22151479899970047No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAGCTTAGTG3170.22081821158146242No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTGCAGACCTAGCG3040.21176257514436775No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACTCGACCAGAA2760.1922581274337023No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTTCTCGACCAGAA2590.1804161413236554No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA2530.1762366168142271No Hit
CCTGTTTGCTCCCCTAGCTTTCGAATATCAGCGTCAGGACCAGTCCAGAA2500.17414685455951295No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATCAGTGTCAGTTACGGACCAGGA2500.17414685455951295No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCGGCTCAGTA2490.1734502671412749No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCGGTCTAGGA2480.17275367972303685No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC2470.1720570923047988No Hit
CCCGTTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAACATCCCAGAA2320.161608281031228No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGAAACGGTCCAGGA2280.15882193135827583No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2280.15882193135827583No Hit
CCCATTCGCTACCCTAGCTTTCGTGTCTGAGCGTCAGGAATGGTCCAGGA2270.15812534394003774No Hit
CCCGTTTGCTACCCTAGCCTTCGCGTCTGAGCGTCAGGAACATCCCAGAA2200.1532492320123714No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAGCAGTCCAGGA2190.15255264459413334No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATGAGCGTCAGTAATAGTTTAGTA2100.14628335782999088No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCAGCGCCAGAT2070.14419359557527672No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGTGTCAGAACTGGTCCAGGA2000.13931748364761035No Hit
CCTGTTTGCTACCCACGCTCTCGCGTCTCAGCGTCAGTATAGGGCCAGAA2000.13931748364761035No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1990.13862089622937232No Hit
CCCATTCGCTACCCTAGCTTTCGCGTCTTAGCGTCAGAAACGGTCCAGAA1990.13862089622937232No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGAACGGTCCAGGA1970.1372277213928962No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGAG1830.1274754975375635No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1810.12608232270108738No Hit
CCTGTTTGCTCCCCACGCCTTCGCGCCTCAGCGTCAGTATTGGTCCAGGA1770.12329597302813518No Hit
CCCATTCGCTACCCTAGCTTTCGCGTCTCAGCGTCAGAAACGGTCCAGAA1740.121206210773421No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGTTCAGACCTAGCG1710.11911644851870684No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATAGGGCCAGAA1710.11911644851870684No Hit
CCCGTTTGCTACCCTGGCTTTCGCGTCTGAGCGTCAGTTGTGGTCCAGAA1690.11772327368223075No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCCCAGTA1680.11702668626399271No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGGTACGGCCCAGAA1610.11215057433632633No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATACTCAGCTGGCG1580.11006081208161217No Hit
CCTGTTTGCTCCCCACGCTTTCATGCCTCAGTGTCAGTTACAGTCCAGAA1580.11006081208161217No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG1570.10936422466337413No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTATCGGTCCAGAA1520.10588128757218387No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAGTATCCGTCCAGAT1450.1010051756445175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT2500.0226.000024
GTTCGAT3650.0226.000024
TGTTCGA3700.0226.000023
CTGTTCG6650.0226.000022
CCGTTCG10000.0226.000022
TATTTGC252.6047928E-9226.03
TGTTTGA2400.0226.03
GTTTGCG152.0928948E-5226.04
CCGATCG100.0018780216226.02
CTTTTTG302.910383E-11226.02
CTTTTGC202.3285793E-7226.03
TTGCTGC152.0928948E-5226.06
TTGCTCG100.0018780216226.06
CGGTTTG500.0226.02
TTGCTAC11050.0226.06
TGTGCTC100.0018780216226.05
TGGTTGC350.0226.03
GACTTCA100.0018780216226.06
CCCGTTC10050.0226.01
GTTCGTG252.6047928E-9226.04
Produced by FastQC (version 0.11.9)