FastQCFastQC Report
Wed 30 Nov 2022
4061-JW28trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW28trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139902
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG5268337.65707423768066No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG63164.514588783577075No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC54243.876999614015525No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC41892.9942388243198814No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG24261.7340709925519293No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG19981.42814255693271No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG19141.3681005275121156No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA16301.1651012851853442No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC14701.0507355148604023No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG14111.00856313705308No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA11920.8520249889208161No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC10890.7784020242741347No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA9770.6983459850466756No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG9110.651170104787637No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG8850.632585667109834No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA7840.5603922745922145No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA7830.5596774885276836No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG7240.5175051107203614No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA6690.4781918771711626No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA6600.47175880259038466No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG5650.4038541264599505No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG5610.400994982201827No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG5350.38241054452402395No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG4970.3552486740718503No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA4940.35310431587825764No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG4800.34309731097482526No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG4730.33809380852310905No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG4710.3366642363940473No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC4450.31807979871624426No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG4450.31807979871624426No Hit
TGGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGA3890.2780517791025146No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG3720.2659004160054895No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC3670.2623264856828351No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC3400.2430272619405012No Hit
ACGCTTTCGTCGATAACATAGAGGTCGTCGCCCGGAGTGATGGCCGTAAC3370.24088290374690854No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA3340.23873854555331592No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC3220.23016111277894524No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA3150.22515761032722909No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA3140.22444282426269815No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG3070.219439321810982No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT3000.21443581935926578No Hit
TGGGGAATCTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG2820.20156967019770983No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG2540.18155566039084503No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG2400.17154865548741263No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2380.17011908335835085No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA2340.1672599391002273No Hit
AGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGGATG2300.16440079484210376No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT2290.16368600877757286No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2280.16297122271304199No Hit
TCGAGAAGCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGA2200.1572529341967949No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA2160.15439378993867137No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC2100.15010507355148606No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC2100.15010507355148606No Hit
TGAGGAATATTGCGCAATGGACGAATGTCTGACGCAGCAACGCCGCGTGG2060.1472459292933625No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG2010.14367199897070806No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG2000.14295721290617716No Hit
TGAGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1990.14224242684164629No Hit
TGAGGAATATTGGTCAATGGTCGCAAGACTGAACCAGCCATGCCGCGTGC1930.13795371045446098No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGT1930.13795371045446098No Hit
CTAAGAATCTTCCGCAATGGGGGAAACCCTGACGGAGCGACGCCGCGTGG1910.1365241383253992No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG1840.131520635873683No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT1760.12580234735743592No Hit
TAGGGAATATTAGTTAATGTGCGAAAGCGCGAACTAGCAACGCCGCGTGT1740.12437277522837414No Hit
GCGCGAAAACTTTACAATGCCGGCAACGGCGATAAGGGGACCTCGAGTGC1640.11722491458306529No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1620.11579534245400352No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC1600.11436577032494175No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG1560.1115066260668182No Hit
TGAGGAATATTGGGCAATGCCCGTAAGGGTGACCCAGCAACGCCGCGTGG1560.1115066260668182No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGGGG1510.10793269574416377No Hit
TGAGGAATATTGCGCAAGGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1500.10721790967963289No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA1480.10578833755057111No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1480.10578833755057111No Hit
TCGAGAATATTCCACAATGGACGAATGTCTGATGGAGCGACGCCGCGTGC1460.10435876542150935No Hit
TGGGGAATATTGCGCAATAGGGGAAACCCTGACGCAGCGACGCCGCGTGG1440.10292919329244757No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA1420.1014996211633858No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1420.1014996211633858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCT152.0204987E-5228.000028
TTTCCAC302.7284841E-11228.000029
AATATTA302.7284841E-11228.000026
CTTGGAC302.7284841E-11228.000029
ATTCCCC152.0204987E-5228.000029
TCGTCGA302.7284841E-11228.000027
TATAGAG152.0204987E-5228.000028
ATCATTC152.0204987E-5228.000027
ATATTAC152.0204987E-5228.000027
ATTTAGC100.001829089228.09
ACTATTG350.0228.06
CTTCCAC202.2282984E-7228.09
TCTTCCA202.2282984E-7228.08
GAGTAAT100.001829089228.02
ATTACGC100.001829089228.09
ATTCGAC100.001829089228.09
TGTTGCG100.001829089228.08
AATTGTT100.001829089228.06
ATATAGA100.001829089228.07
TTAAGAA252.4701876E-9228.01
Produced by FastQC (version 0.11.9)