FastQCFastQC Report
Wed 30 Nov 2022
4061-JW29trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW29trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136337
Sequences flagged as poor quality0
Sequence length234
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG5945943.611785502101405No Hit
TGAGGAATATTGCGCAATGGACGAAAGTCTGACGCAGCAACGCCGCGTGG69725.113798895384232No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC65844.829209972347932No Hit
TGAGGAATATTGCGCAATGGGCGAATGCCTGACGCAGCAACGCCGCGTGG22891.678927950593016No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGC18771.3767355890183883No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG17681.2967866389901495No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG17511.2843175366921673No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA17511.2843175366921673No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGTGTGA14091.0334685375209958No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG11980.8787049737048638No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA10670.7826195383498243No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG8930.6549946089469476No Hit
GGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGGATG7680.5633100332264902No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA7330.5376383520247622No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA6940.5090327643999795No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA6700.4914293258616516No Hit
TAGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCAACGCCGCGTGC6670.48922889604436065No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGC6010.4408194400639592No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT5710.4188151418910494No Hit
TGAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATCCCGCGTGA5600.4107468992276491No Hit
TCGAGAATATTCCACAATGGACGAAAGTCTGATGGAGCGACGCCGCGTGC5390.3953438905066123No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGA5290.38800912444897573No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA5260.3858086946316847No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG4580.3359322854397559No Hit
TAAGGGATATTGCGCAAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG4270.31319451066108245No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGC4240.3109940808437915No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG4000.29339064230546363No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAATGCCGCGTGC3970.2911902124881727No Hit
TGAGGAATATTGGGCAATGCCCGCAAGGGTGACCCAGCAACGCCGCGTGG3800.27872111019019047No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGG3610.264785054680681No Hit
TAAGGGATATTGCGACAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG3590.26331810146915363No Hit
TGAGGAATATTGGTCAATGGAGGCAACTCTGAACCAGCCATGCCGCGTGA3480.2552498588057534No Hit
TGGGGAATCTTGGGCAATGGGCGAAAGCCTGACCCAGCGACGCCGCGTGG3470.25451638219998973No Hit
TGAGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA3460.2537829055942261No Hit
TAGGGAATATTGGTTAATGGACGAAAGTCTGAACCAGCAACGCCGCGTGT3440.25231595238269877No Hit
TGAGGAATATTGGACAATGGATGGAAATCTGATCCAGCCATGCCGCGTGC3340.24498118632506216No Hit
TAAGGGATATTGCGCGAATGGACGAAAGTCTGACGCAGCAACGCCGCGTG3270.23984685008471654No Hit
CGCCGCGTGGATGATGAAGTTCTTCGGAATGTAAAATCCTTTTGCAGAGG3250.23837989687318922No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC2980.21857602851757044No Hit
TAGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGT2950.21637559870027945No Hit
TGAGGAATATTGGGCAATGCCCGAAAGGGTGACCCAGCAACGCCGCGTGG2530.18556958125820577No Hit
TGAGGAATATTGCGCAATGGACGAATGTCTGACGCAGCAACGCCGCGTGG2450.17970176841209648No Hit
TAGGGAATATTGCACAATGGGCGAATGCCTGATGCAGCAACGCCGCGTGC2350.1723670023544599No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCGACGCCGCGTGG2340.17163352574869623No Hit
TGAGGAATATTGCGCAATGGCCGAAAGGCTGACGCAGCGACGCCGCGTGA2120.15549704042189574No Hit
TGAGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA2090.15329661060460475No Hit
TAGGGAATATTGCACCATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA2070.15182965739307747No Hit
TGAGGAATCTTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGA1970.14449489133544086No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCGACGCCGCGTGG1930.14156098491238622No Hit
CAAGGAATTTTCCCCAATGGCCGAAAGGCTGAGGGAGCAACGCCGCGTGG1910.14009403170085888No Hit
TAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGC1890.1386270784893316No Hit
TGAGGAATATTGGTCAATGGTCGCAAGACTGAACCAGCCATGCCGCGTGC1850.13569317206627696No Hit
TGCGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1730.12689145279711306No Hit
TGAGGAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGG1720.12615797619134939No Hit
TGGGGAATTTTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1670.12249059316253108No Hit
TGAGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1610.11808973352794912No Hit
TGAGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1580.11588930371065816No Hit
TAAGGGATATTGCGACAATGGACGCAAGTCTGACGCAGCAACGCCGCGTG1550.11368887389336718No Hit
TGGGGAATTGTTCGCAATGGGCGCAAGCCTGACGACGCAACGCCGCGTGG1540.11295539728760351No Hit
CAGTGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCG1510.11075496747031253No Hit
TGAGGAATATTGGTCAATGGGCGAAAGCCTGAACCAGCCATCCCGCGTGC1510.11075496747031253No Hit
TAAGGGATATTGCGCCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTG1490.10928801425878522No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG1480.10855453765302155No Hit
TGGGGAATATTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA1470.1078210610472579No Hit
TAGGGAATATTGCATAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGT1460.10708758444149424No Hit
TAGGGAATTTTCGGCAATGGGGGGAACCCTGACCGAGCGATGCCGCGTGA1450.10635410783573057No Hit
GAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGT1430.10488715462420325No Hit
TAGGGAATATTGGTTAATGTGCGAAAGCGCGAACCAGCAACGCCGCGTGT1420.10415367801843961No Hit
TGAGGAATATTGGTCAATGGGCGAGAGCCTGAACCAGCCATGCCGCGTGC1400.10268672480691228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA100.0018290374228.03
ACTATTG202.2280983E-7228.06
GAGGACT100.0018290374228.02
CATATTG152.0203854E-5228.06
ATTAGTA100.0018290374228.09
AATCATG100.0018290374228.06
CTTCCAC100.0018290374228.09
AACTTTA350.0228.08
CATTGCG152.0203854E-5228.08
ATTACGC100.0018290374228.09
ACATTGC152.0203854E-5228.07
AGGACTA100.0018290374228.03
ATTGTTC202.2280983E-7228.07
ACTTCGC100.0018290374228.09
TATATTG152.0203854E-5228.06
TTTCCAC202.2280983E-7228.09
AATATTT152.0203854E-5228.06
AATATTA400.0228.06
AATATAG152.0203854E-5228.06
CTTGGAC252.4701876E-9228.09
Produced by FastQC (version 0.11.9)