FastQCFastQC Report
Wed 30 Nov 2022
4061-JW31trimR_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW31trimR_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113826
Sequences flagged as poor quality0
Sequence length234
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGG1622914.257726705673571No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGA1207710.610053941981622No Hit
TGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC95658.403176778591886No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA61105.367842145028376No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATACCGCGTGA45153.9665805703442096No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATTCCGCGTGA28242.4809797410082055No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGA26902.363256198056683No Hit
GGAATTTTCGGCAATGGGCGCAAGCCTGACCGAGCAACGCCGCGTGGAGG24182.124294976543145No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATACCGCGTGT23902.099696027269692No Hit
CTAAGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGG15841.3915977017553107No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA15501.3617275490661185No Hit
TGGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGA12851.1289160648709433No Hit
TGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGG12021.0559977509532092No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA10940.9611160894698927No Hit
TTTCGAATCATTCACAATGGGCGAAAGCCTGATGGTGCGACGCCGCGTGA10270.9022543179941315No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGC9470.8319716057842673No Hit
TGGGGAATATTGGACAATGGGGGCAACCCTGATCCAGCAATGCCGCGTGA7250.6369370794018941No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATACCGCGTGT6880.604431325004832No Hit
CAAGGAATTTTCGGCAATGGGCGCATGCCTGACCGAGCAACGCCGCGTGG6550.5754397062182629No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAACGCCGCGTGG6470.5684114349972765No Hit
TCTAGAGGCTTCGTCAATGGGGGAAACCCTGAACGAGCGACGCCGCGTGC6320.555233426457927No Hit
TGGGGAATCTTGCGCAATGGACGAAAGTCTGACGCAGCCACGCCGCGTGA6270.5508407569448105No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGA6160.5411768840159542No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATTCCGCGTGA5890.5174564686451251No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA4650.40851826471983554No Hit
TCGAGGATCTTCGGCAATGCGCGCAAGCGCGACCGAGCGACGCCGCGTGG4610.40500412910934236No Hit
TGGGGAATCTTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGA4590.4032470613040957No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAAGACCGCGTGA4180.3672271712965403No Hit
TAAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGG3950.34702089153620436No Hit
TGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGA3890.3417496881204646No Hit
TCGAGAATAATTCACAATGGGGGCAACCCTGATGGTGCAACGCCGCGTGG3780.33208581519160824No Hit
TGAGGAATATTGGTCAATGGGCGGATGCCTGAACCAGCCATGCCGCGTGC3740.328571679581115No Hit
TTAAGAATCTTGCGCAATGGAGGCAACTCTGACGCAGCGACGCCGCGTGT3720.32681461177586846No Hit
TGGGGAATTTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA3660.3215434083601286No Hit
TCGAGAATTTTTCTCAATGGGGGAAACCCTGAAGGAGCGACGCCGCGTGG3330.29255178957355965No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG3230.28376645054732663No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA3200.2811308488394567No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCGCGTGA3020.26531723859223727No Hit
GCGCGAAAACTTTACAATGCGGGCAACCGTGATAAGGAAACCCCGAGTGC3010.26443870468961395No Hit
TAAGGAATATTGCGCAATGGGCGACAGCCTGACGCAGCGACGCCGCGTGG2970.26092456907912076No Hit
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGA2910.2556533656633809No Hit
TGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGA2870.25213923005288774No Hit
TGGGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCGACGCCTCGTGA2790.24511095883190132No Hit
TGGGGAATATTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA2630.23105441638992852No Hit
TGGGGAATATTGGACAATGGGCGCATGCCTGATCCAGCCATGCCGCGTGA2520.22139054346107215No Hit
TGGGGAATTTTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGA2410.21172667053221583No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGA2360.20733400101909932No Hit
TAGGGGATATTGGTGAATGGGCGAAAGCCTGAACCAGCAACGCCGCGTGT2350.20645546711647603No Hit
TGGGGAATCTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGG2280.20030572979811292No Hit
TGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGT2050.18009945003777697No Hit
TAGGGAATATTGGTTAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA2000.17570678052466043No Hit
TGGGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGC1920.16867850930367403No Hit
TTAGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCGACGTCGCGTGA1870.1642858397905575No Hit
TGAGGAATATTGGTCAATGGGGGCAACCCTGAACCAGCCATGCCGCGTGG1860.16340730588793423No Hit
TCGAGAATTTTTCACAATGGGCGAAAGCCTGATGGAGCGACGCCGCGTGG1710.15022929734858467No Hit
TGGGGAATTTTTCGCAATGGGGGAAACCCTGACGAAGCAACGCCGCGTGG1690.14847222954333808No Hit
TCGAGAATCATTCGCAATGGGCGAAAGCCTGACGGTGCGACGCCGCGTGG1680.14759369564071476No Hit
TGGGGAATTTTCCGCAATGGGCGAATGCCTGACGGAGCAATACCGCGTGA1670.14671516173809146No Hit
CAAGGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGG1610.14144395832235165No Hit
TGAGGAATCTTGCGCAATGGGGGAAACCCTGACGCAGCAACGCCGCGTGA1590.13968689051710506No Hit
TGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCCATGCCGCGTGA1580.13880835661448174No Hit
TGGGGAATTTTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1470.12914448368562542No Hit
TAGGGAATATTGCTTAATGGGCGAAAGCCTGAAGCAGCAACGCCGCGTGT1460.12826594978300213No Hit
TGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGC1430.12563034807513224No Hit
TGAGGAATATTGCGCAATGGGCGAAAGCCTGACGCAGCAACGCCGCGTGG1420.12475181417250891No Hit
TGGGGAATATTGGGCAATGGGCGAAAGCCTGACCCAGCAACGCCGCGTGA1410.12387328026988562No Hit
AGAATATTCCGCAATGGACGAAAGTCTGACGGAGCGACGCCGCGTGGATG1380.12123767856201571No Hit
TAACGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGT1370.12035914465939242No Hit
TGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGC1340.1177235429515225No Hit
GGAATTTTCCCCAATGGGCGCAAGCCTGAGGGAGCAACGCCGCGTGCAGG1310.1150879412436526No Hit
GCGCGAAAACTTTGCAATGCGGGAAACCGTGACAGGGGAACTCCGAGTGC1290.11333087343840598No Hit
TTAAGAATCTTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGA1260.11069527173053607No Hit
TGCTGAATTATTCGCCAATGCGCGAAAGCGTGACGGTGCGACGCCGCGTG1230.10805967002266617No Hit
GGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAAGACCGCGTGAGGG1230.10805967002266617No Hit
TCGAGAATTTTTCACAATGGGCGCAAGCCTGATGGAGCGACGCCGCGTGG1220.10718113612004287No Hit
TAGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGCGTGC1190.10454553441217296No Hit
TCGAGAATCTTCGGCAATGGGCGAAAGCCTGACCGAGCGACGCCGCGTGT1180.10366700050954966No Hit
CAAGGAATTTTCCCCAATGGGCGACAGCCTGAGGGAGCAACGCCGCGTGG1160.10190993270430306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATCT5500.0228.000024
TATTGCA1150.0228.000028
ATTTTGC4250.0228.000027
ATTGCAC1150.0228.000029
ACTATTG252.4683686E-9228.06
TCTTGGA950.0228.08
AGTTTTC100.001828642228.06
GAGCTTC100.001828642228.06
CTTCCGC2200.0228.09
CTTCCAC100.001828642228.09
TCTTCCA100.001828642228.08
GAACTTT202.2264976E-7228.05
AGGAACT100.001828642228.03
GCTCTTC152.0195117E-5228.06
GCATTTT202.2264976E-7228.05
AATTGTT252.4683686E-9228.06
GAGAATC1200.0228.03
CTTGCAC950.0228.09
TATTCGC202.2264976E-7228.09
CGAGGAT650.0228.02
Produced by FastQC (version 0.11.9)