FastQCFastQC Report
Wed 30 Nov 2022
4061-JW31trimR_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW31trimR_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113826
Sequences flagged as poor quality0
Sequence length232
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA1826316.044664663609367No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGTGTCAGTTATGGTCCAGT1223610.749740832498727No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCGATGTCGGCCCGGCG103309.075255214098712No Hit
CCCATTTGCTACCCTAGCTTTCGTCTCTGAGTGTTAGTAATAGCCCAGTA48344.2468328852810435No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTACCGGGCCAGTG15581.3687558202871049No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTGAGTCCAGAG13691.2027129126913008No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGTGTCAGACATCGTCCAGAA13641.1983202431781843No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG12761.1210092597473336No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG12511.099045912181751No Hit
CCGGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGATAAGGCCCAGGT12241.075325496810922No Hit
CCTGTTTGCTCCCCGCACTTTCGTACCTCAGCGTCAGTCATTGGCCAGGA12161.0682972255899355No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGCGTCAGTACCGGGCCAGTG12001.0542406831479627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT11901.0454553441217298No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAATCGACGGCCAGGT11791.0357914711928733No Hit
CCCTTTCGCTCCCCTAGCTTTCGTCCCTCAGTGTCAGTACAGGTCCAGCA10980.964630225080386No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTCTAGTCCAGGG10050.8829265721364188No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCGACCCAGGG9770.8583276228629663No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTTTAGTCCAGGG8570.75290355454817No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG7620.6694428337989563No Hit
CCCTTTGGCTACCCACGCTTTCGTGCCTGAGCGTCAGCAATTGTCCAGGA7490.6580218930648534No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC6820.5991601215890922No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGAACCGGTCCAGGT6720.590374782562859No Hit
CCTGTTTGCTCCCCACGCCATCGTGGCTCAGTGTCAGGTCTCGCCCAGAA6630.5824679774392494No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGAAATGGGCCAGCA6200.5446910196264474No Hit
CCGGTTTGCTCCCCCAGCTTTCGTCCCTCACTGTCGGAGCCGTTCTGGTG5950.5227276720608649No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG5730.5033999262031521No Hit
CCTGTTTGCTCCCCACGCTTTCGTAGTTGAGTGTCAGTATCTGTCCAGGT5410.4752868413192065No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGGCCAGGT4910.4313601461880414No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGATGGGGTCCAGCG4500.395340256180486No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTG4490.39446172227786275No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCAGTCCAGAT4320.37952664593326657No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG4200.36898423910178696No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGT4190.36810570519916364No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAATATTATCCCAGGG4170.36634863739391704No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGAGCCAGTG4150.3645915695886704No Hit
CCCATTCGCTCCCCTAGCTTTCGTCCTGTGAGGGTCAGTTATGGTCCAGT4020.3531706288545675No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTCTCCGTCCAGCA3810.33472141689947815No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG3770.3312072812889849No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3670.32242194226275195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGAT3490.3066083320155325No Hit
CCTGTTTGCTCCCCACACTTTCGCACCTCAGCGTCAGTACCTGTCCAGGT3270.2872805861578199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATCACGGGCCAGAT3240.28464498444994996No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG3200.2811308488394567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGGTATGGACCAGAG3190.2802523149368334No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTA3050.2679528403001072No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCACTACAAGTCCAGCA2950.25916750127387417No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCGGTCCAGGC2860.2512606961502645No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG2710.23808268761091492No Hit
CCTGTTTGATCCCCACGCTTTCGCGCTGCAGCGTCAGAAACTGGCCAGTG2580.22666174687681195No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGTCTGAGCGTCAGTCGTGGTCCAGAA2520.22139054346107215No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGTAACTGTCCAGAG2470.21699787394795564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGG2430.21348373833746243No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTCCGAGCCAGTG2330.20469839931122943No Hit
CCCTTTCGCTACCCTGGCCTTCGAGCCTCAGCGTCAGGAGTTGTCCAGTA2320.2038198654086061No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAACAATTTAGTA2300.20206279760335952No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGTCCAGTT2260.1985486619928663No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTGTCGTCCAGGT2230.1959130602849964No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGGTATGTTCCAGAG2210.1941559924797498No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATCGACCCAGGG2170.19064185686925658No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGACCAGTT2150.18888478906401No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGACCAGTT2120.18624918735614007No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGC2110.18537065345351678No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGAA2090.18361358564827016No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAGCTGTCCAGAA2090.18361358564827016No Hit
CCGGTTCGCTCCCCACGCTTTCGTACCTCAGCGTCAGGTCCGACCCAGGT2010.17658531442728376No Hit
CCCTTTGGCTACCCACGCTTTCGTGCCTGAGTGTCAGCAATTGTCCAGGA1960.17219264491416725No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGGTACAGCCCAGAG1960.17219264491416725No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGAAGAGGTCCAGCA1950.17131411101154395No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG1920.16867850930367403No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG1860.16340730588793423No Hit
CCTGTTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTAATGGTCCAGAG1830.1607717041800643No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG1820.15989317027744102No Hit
CCTGTTTGCTCCCCACGCCGTCGTGTCTCAGTGTCAGGTCTCGCCCAGAA1730.1519863651538313No Hit
CCCGTTCGCTACCCTAGCTTTCGCACCTCAGCGTCAGGATATGGCCAGGA1730.1519863651538313No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTT1700.14935076344596138No Hit
CCTTTTCGCTCCCCTGGCCTTCGTCTCTCAGCGTCAGGTGTCGTCCAGAG1690.14847222954333808No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATAACGGGCCAGAT1650.14495809393284487No Hit
CCTGTTTGCTCCCCACGCCGTCGTGGCTCAGCGTCAGGTCTCGCCCAGAA1620.14232249222497498No Hit
CCCGTTTGCTACCCTAGCTTTCGCGTCTGAGCGTCAGTCTCGGGCCAGGA1550.13617275490661185No Hit
CCCGTTCGCTACCCTAGCTTTCGTGCCTCAGCGTCAGAAGAGACCCAGTG1490.13090155149087201No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTTGGCCCAGAT1460.12826594978300213No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCATGAGCGTCAGAAATGCCCCAGCA1440.12650888197775553No Hit
CCGGTTCGCTCCCCACGCTTTCGCACCTCAGCGTCAGGTCAGGCCCAGGT1420.12475181417250891No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAATCATTGGCCAGGA1400.12299474636726232No Hit
CCTGTTTGCTACCCACGCTTTCGCGTCTCAGCGTCAATATCGGTCCAGGT1320.1159664751462759No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAACCCAGGA1310.1150879412436526No Hit
CCTGTTTGCTCCCCACGCCTTCGTGGCTCAGTGTCAGGTCTCGCCCAGAA1300.1142094073410293No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACTGTCCAGGA1300.1142094073410293No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTTCTGGACCAGGA1300.1142094073410293No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTCGGTCCAGAT1290.11333087343840598No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA1280.11245233953578268No Hit
CCTGTTTGCTCCCCACGCCGTCGTGCCTCAGTGTCAGGTCTCGCCCAGAA1270.1115738056331594No Hit
CCGGTTCGCTCCCCACGCTGTCGCACATGAGTGTCAGTTGCTGCCCAGGT1270.1115738056331594No Hit
CCCGTTCGCTCCCCATGCTTTCGCGTCTGAGCGTCAGGTGCAGCCCAGCA1250.10981673782791278No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTCTCGACCAGAA1220.10718113612004287No Hit
CCTGTTTGATCCCCACACTTTCGCACCTCAGCGTCAGTCATCGGCCAGGA1190.10454553441217296No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1160.10190993270430306No Hit
CCCGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATGGCCCAGCA1160.10190993270430306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTC12350.0226.000021
TGTTTGC63150.0226.000023
TTGGCTA1100.0226.000025
TTTGGCT1100.0226.000024
CTGTTCG12300.0226.000022
CGGTTCG650.0226.000022
TGGCTAC1100.0226.000026
TTTGATC2900.0226.000025
CGTTTGC3850.0226.03
CGTTTGA152.0917589E-5226.03
GTTTGCG100.0018775116226.04
GGTTGCT100.0018775116226.04
TGTTTGA2700.0226.03
GGTTCGC600.0226.03
CCGTTCG4250.0226.02
CCTTTTT152.0917589E-5226.01
ATTTGCT5400.0226.04
CTGTTTG65850.0226.02
TGTGTGC100.0018775116226.03
GTTGCTC100.0018775116226.05
Produced by FastQC (version 0.11.9)